LOCUS       sequence-299418-        2272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0213
FEATURES             Location/Qualifiers
     source          1..2272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     72..91
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     319..337
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(341..384)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      632..689
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(713..730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(884..1472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(964..983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1655..1749)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1770..2272,1..157))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
       61 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
      121 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
      181 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
      241 aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc
      301 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc
      361 ttagctttcg ctaaggatga tttctggaat tcggtctcag gagggaggcg tgtcgtttta
      421 gtgaggctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa
      481 agtcgcctaa ggtcactatc agctagcaaa tatttattgg gtacccgaat aactcagcat
      541 cttttgctaa attctatcgt ggctatataa ggttttgcta ttcattgaaa gcagtagtga
      601 ctgatttgta tatatactcg agaccctgca gtccggcaaa aaagggcaag gtgtcaccac
      661 cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca
      721 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      781 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      841 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc
      901 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      961 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1021 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1081 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1141 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1201 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1261 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1321 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1381 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1441 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1501 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1561 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1621 atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc
     1681 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     1741 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac
     1801 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     1861 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     1921 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     1981 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     2041 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     2101 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     2161 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
     2221 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gt
//
