LOCUS       sequence-299419-        2297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0214
FEATURES             Location/Qualifiers
     source          1..2297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(91..185)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(206..865)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     780..799
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1027..1045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1049..1092)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1365..1422
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1446..1463)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1617..2205)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1697..1716)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
       61 gtaaacttgg tctgacagct cgaggcttgg attctcacca ataaaaaacg cccggcggca
      121 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
      181 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
      241 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
      301 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
      361 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
      421 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
      481 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
      541 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
      601 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
      661 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
      721 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
      781 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
      841 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
      901 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
      961 gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     1021 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
     1081 aaggatgatt tctggaattc ggtctcagga gggaggcgtg tcgttttagt gaggctggag
     1141 tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg
     1201 tcactatcag ctagcaaata aaagcccagg tcgtgttgat tcggattttg taaaatgggt
     1261 tctggcgtct ttatggaggt tctccatctg ttttactata taaggttttg ctattcattg
     1321 aaagcagtag tgactgattt gtatatatac tcgagaccct gcagtccggc aaaaaagggc
     1381 aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttatgcag
     1441 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1501 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1561 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1621 cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1681 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1741 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1801 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1861 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1921 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1981 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2041 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2101 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2161 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2221 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2281 agattatcaa aaaggat
//
