LOCUS       sequence-299420-        2268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0395
FEATURES             Location/Qualifiers
     source          1..2268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     208..227
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     455..473
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(477..520)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      764..821
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(845..862)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1016..1604)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1096..1115)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1787..1881)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1902..2268,1..293))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
       61 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
      121 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
      181 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
      241 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
      301 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
      361 ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta
      421 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt
      481 cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgg tctcaggagg
      541 gaggcgtgtc gttttagtga ggcaaaactg gttctctctg ccgacagtgg tcccaaatgt
      601 aggctattgc acctcacaac cacgtctaca aagcaagtgg attacagggc tcctgctagg
      661 agctgacgta agggatgacg cacaagcaag acctctatat aaggttttgc tattcattga
      721 aagcagtagt gactgatttg tatatatact cgagaccctg cagtccggca aaaaagggca
      781 aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg
      841 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      901 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      961 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1021 acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1081 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1141 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1201 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1261 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1321 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1381 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1441 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1501 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1561 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1621 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1681 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     1741 tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa
     1801 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
     1861 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
     1921 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     1981 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     2041 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     2101 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
     2161 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     2221 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtt
//
