LOCUS       sequence-299421-        2268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0396
FEATURES             Location/Qualifiers
     source          1..2268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     157..176
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     404..422
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(426..469)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      713..770
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(794..811)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(965..1553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1045..1064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1736..1830)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1851..2268,1..242))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
       61 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
      121 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
      181 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
      241 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
      301 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc
      361 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
      421 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcggt
      481 ctcaggaggg aggcgtgtcg ttttagtgag gggaccgatg ctctctctgc cgacagtggt
      541 cccaaacaca tgtaggacat gtaggcaacc acgtctacaa agcaagtgga tgtctgcacc
      601 tctctacaaa actgacgtaa gggatgacgc acacttgatg atactatata aggttttgct
      661 attcattgaa agcagtagtg actgatttgt atatatactc gagaccctgc agtccggcaa
      721 aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg
      781 ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      961 cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1381 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct
     1741 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     1801 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
     1861 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     1921 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     1981 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     2041 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     2101 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     2161 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     2221 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagc
//
