LOCUS       sequence-299422-        2208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0451
FEATURES             Location/Qualifiers
     source          1..2208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     100..119
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     347..365
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(369..412)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      596..653
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(677..694)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1619..1713)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1734..2208,1..185))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
       61 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
      121 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
      181 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
      241 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc
      301 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
      361 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
      421 ggtctcagga gggaggcgtg tcgttttagt gaggaaccac gtctacaact gacgtaaggg
      481 atgacgcaca agcaagtgga ttctctctgc cgacagtggt cccaaactat ataaggtttt
      541 gctattcatt gaaagcagta gtgactgatt tgtatatata ctcgagaccc tgcagtccgg
      601 caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc
      661 gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      841 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1261 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat
     1621 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     1681 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     1741 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     1801 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     1861 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     1921 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
     1981 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     2041 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     2101 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     2161 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaaga
//
