LOCUS       sequence-299426-        2231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0453
FEATURES             Location/Qualifiers
     source          1..2231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     98..117
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     345..363
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(367..410)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      617..674
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(698..715)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(869..1457)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(949..968)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1640..1734)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1755..2231,1..183))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
       61 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
      121 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
      181 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
      241 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac
      301 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
      361 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgg
      421 tctcaggagg gaggcgtgtc gttttagtga gggtgggagc caccatgaag ataagataat
      481 aatgttgaag ataagaatga cgtaagccat gacgtctagc tttgtcaaaa gctaaaaaag
      541 atgatgccta tataaggttt tgctattcat tgaaagcagt agtgactgat ttgtatatat
      601 actcgagacc ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt
      661 taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct
      721 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      781 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      841 accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc
      901 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      961 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1021 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1081 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1141 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1201 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1261 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1321 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1381 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1441 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1501 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1561 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1621 ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     1681 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     1741 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     1801 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     1861 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     1921 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     1981 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     2041 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     2101 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     2161 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     2221 cgggcaagaa t
//
