LOCUS       sequence-299427-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses synonymized MS2 coat protein dimer (MCP) fused to 
            hyperactive ADAR catalytic domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Biswas J, Rahman R, Gupta V, Rosbash M, Singer RH
  TITLE     MS2-TRIBE Evaluates Both Protein-RNA Interactions and Nuclear 
            Organization of Transcription by RNA Editing.
  JOURNAL   iScience. 2020 Jun 28;23(7):101318. doi: 10.1016/j.isci.2020.101318.
  PUBMED    32674054
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-06-28"; volume: "23(7):101318. doi"; pages: " 
            10.1016/j.isci.2020.101318"
COMMENT     Sequence Label: pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2616
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      2624..2633
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2636..2656
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2660..2686
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2660..2686
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2687..2710
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4679..4720
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4748..4804
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4832..5545
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             4832..5542
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    5559..6147
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5612..5632)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6030..6041)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6222..6455
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6574..6592)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6660..6764
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6765..6833,6834..7625)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6983..7002)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7796..8384
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8285..8304
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8538..8555
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8795..8816
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8831..8836,8837..8854,8855..8861)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8869..8885
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8874..8896
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8893..8909
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8893..8909
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8959..8978
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgcgccacca tgggcccaaa
     2641 aaagaaaaga aaagttggct acccctacga cgtgcccgac tacgccctgg aagttctgtt
     2701 ccaggggccc ctgttattaa ttaacgcttc taactttact cagttcgttc tcgtcgacaa
     2761 tggcggaact ggcgacgtga ctgtcgcccc aagcaacttc gctaacggga tcgctgaatg
     2821 gatcagctct aactcgcgtt cacaggctta caaagtaacc tgtagcgttc gtcagagctc
     2881 tgcgcagaat cgcaaataca ccatcaaagt cgaggtgcct aaaggcgcct ggcgttcgta
     2941 cttaaatatg gaactaacca ttccaatttt cgccacgaat tccgactgcg agcttattgt
     3001 taaggcaatg caaggtctcc taaaagatgg aaacccgatt ccctcagcaa tcgcagcaaa
     3061 ctccggcatc tacgccatgg ccagcaactt cacccagttc gtgctggtgg acaacggcgg
     3121 caccggcgac gtgaccgtgg cccccagcaa cttcgccaac ggcatcgccg agtggatcag
     3181 cagcaacagc agaagccagg cctacaaggt gacctgcagc gtgagacaga gcagcgccca
     3241 gaacagaaag tacaccatca aggtggaggt gcccaagggc gcctggagaa gctacctgaa
     3301 catggagctg accatcccca tcttcgccac caacagcgac tgcgagctga tcgtgaaggc
     3361 catgcagggc ctgctgaagg acggcaaccc catccccagc gccatcgccg ccaacagcgg
     3421 catctacgcc gacccaccgg gaggtaccat ggactacagt ccaatggtgg tgccacagaa
     3481 gaacgtaccc ctgccaatcg acgacaagtc gtcatcgatg gagttgcctc agatacacgc
     3541 ggatacgatt ggtcggttgg tcttagaaaa gttcatggaa gtaatcaagg gccaggaggc
     3601 ttactcgcgt cgaaaggtat tagcgggcat tgtaatgact gaaaacatga atttttgtga
     3661 agccaaagtt atttcagttt cgacgggcac caagtgtgtc agcggtgagc atatgagtgt
     3721 gaacggagct gtcctaaatg attcccatgc tgaaatagtc tccaggcgtt gtctcctcaa
     3781 atatttatat gcacagctgg accttcagtg caatcaggcc acagcatatc agtcgatttt
     3841 cgtgaggaat actgatgggc aataccctta taaactaaaa tccggggtac atttccattt
     3901 gtatataaat acagcacctt gtggggatgc acggatattt tctcctcacg aaaacgacac
     3961 tggtgttgat aaacatccaa atagaaaagc tcgaggccag ctgcgcacca aaatcgagtc
     4021 cggtcagggg acgattccag tgaaaagcag tgatggtatc cagacgtggg atggcgtatt
     4081 gcagggccaa cgcctgctaa caatgtcgtg ctcggataaa attgcccgtt ggaacatcgt
     4141 gggcatccaa ggctcactat tgtcttccat aattgaaccg gtgtacctgc attcgattgt
     4201 gctgggaagc ctgttgcatc cggagcacat gtaccgcgca gtttgcggcc gaattgagaa
     4261 gtccattcaa ggcctgccac cgccgtacca tctgaacaag ccgcgcctgg cactagtcac
     4321 ttcggccgag ccgcggaatc aggccaaggc tcccaacttc gggatcaatt ggaccattgg
     4381 ggacaccgag ctggaagtgg tgaactcgct aaccggtcga accattggcg gccaggtgtc
     4441 tcgcatcaca aagcaggcgt ttttcgttaa gtatggattt ctaatggcaa acttgcccgg
     4501 tattttagtc cgcaaagtaa ccactgacta tgggcaaacc aaagctaacg ttaaggacta
     4561 tcagatcgca aagctagaat tgttctctgc attcaagcga gaagaccttg gcagctggct
     4621 gaagaaaccc attgaacaag acgagttcgg tcttgccgaa tctagagggc ccttcgaagg
     4681 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggta acggaccggg
     4741 cagcggcgcc accaacttca gcctgctgaa gcaggccggc gacgtggagg agaaccccgg
     4801 ccccattgca tgcggttcag gttcatcagg ctctaaaggt gaagaattat tcactggtgt
     4861 tgtcccaatt ttggttgaat tagatggtga tgttaatggt cacaaatttt ctgtctccgg
     4921 tgaaggtgaa ggtgatgcta cttacggtaa attgacctta aaatttattt gtactactgg
     4981 taaattgcca gttccatggc caaccttagt cactacttta acttatggtg ttcaatgttt
     5041 ttctagatac ccagatcata tgaaacaaca tgactttttc aagtctgcca tgccagaagg
     5101 ttatgttcaa gaaagaacta tttttttcaa agatgacggt aactacaaga ccagagctga
     5161 agtcaagttt gaaggtgata ccttagttaa tagaatcgaa ttaaaaggta ttgattttaa
     5221 agaagatggt aacattttag gtcacaaatt ggaatacaac tataactctc acaatgttta
     5281 catcatggct gacaaacaaa agaatggtat caaagttaac ttcaaaatta gacacaacat
     5341 tgaagatggt tctgttcaat tagctgacca ttatcaacaa aatactccaa ttggtgatgg
     5401 tccagtcttg ttaccagaca accattactt atccactcaa tctgccttat ccaaagatcc
     5461 aaacgaaaag agagaccaca tggtcttgtt agaatttgtt actgctgctg gtattaccca
     5521 tggtatggat gaattgtaca aataaatcga tagatcctaa tcaacctctg gattacaaaa
     5581 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5641 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     5701 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5761 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5821 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5881 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5941 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     6001 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     6061 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     6121 ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc
     6181 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     6241 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     6301 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6361 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6421 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     6481 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa
     6541 ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     6601 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     6661 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6721 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6781 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6841 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6901 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6961 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     7021 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7081 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7141 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7201 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7261 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7321 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7381 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7441 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7501 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7561 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7621 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7681 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7741 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7801 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7861 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7921 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7981 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8041 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8101 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8161 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8221 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8281 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8341 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8401 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8461 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8521 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8581 aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat
     8641 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8701 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc
     8761 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     8821 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8881 aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt
     8941 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     9001 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
     9061 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
     9121 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
