LOCUS       sequence-299429-        2231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0454
FEATURES             Location/Qualifiers
     source          1..2231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(24..67)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      274..331
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(355..372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(526..1114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(606..625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1297..1391)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1412..2071)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1986..2005
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
       61 tgatttctgg aattcggtct caggagggag gcgtgtcgtt ttagtgaggt gaagataaga
      121 taataatgtt gaagataaga atgacgtaag ccatgacgtc tagctttgtc aaaagctaaa
      181 aaagatgatg cgtgggagcc accactatat aaggttttgc tattcattga aagcagtagt
      241 gactgatttg tatatatact cgagaccctg cagtccggca aaaaagggca aggtgtcacc
      301 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
      361 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      421 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      481 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg
      541 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      601 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      661 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      721 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      781 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      841 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      901 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      961 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1021 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1081 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1141 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1201 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1261 atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg
     1321 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     1381 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt
     1441 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     1501 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     1561 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     1621 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     1681 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     1741 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     1801 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     1861 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     1921 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     1981 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     2041 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     2101 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     2161 ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca
     2221 ttaacctata a
//
