LOCUS       sequence-299431-        2224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0402
FEATURES             Location/Qualifiers
     source          1..2224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     87..105
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(109..152)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      352..409
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(433..450)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(604..1192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(684..703)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1375..1469)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1490..2149)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2064..2083
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca
       61 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
      121 aaaaatcctt agctttcgct aaggatgatt tctggaattc ggtctcagga gggaggcgtg
      181 tcgttttagt gaggagctta gcaaatgagc taagcacata cgtcagaaac cattattgcg
      241 ctaggaggac cacagggctc atcactatca gctgcccaat tactatataa ggttttgcta
      301 ttcattgaaa gcagtagtga ctgatttgta tatatactcg agaccctgca gtccggcaaa
      361 aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt
      421 tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      481 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      541 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      601 gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      661 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      721 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      781 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      841 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      901 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      961 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1021 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1081 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1141 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1201 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1261 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1321 taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc
     1381 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     1441 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
     1501 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     1561 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     1621 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     1681 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
     1741 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     1801 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     1861 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     1921 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     1981 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     2041 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     2101 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     2161 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     2221 ggtg
//
