LOCUS       sequence-299432-        2182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0455
FEATURES             Location/Qualifiers
     source          1..2182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     69..88
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     316..334
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(338..381)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      539..596
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(620..637)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(791..1379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(871..890)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1562..1656)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1677..2182,1..154))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
       61 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
      121 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
      181 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
      241 ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg
      301 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
      361 gctttcgcta aggatgattt ctggaattcg gtctcaggag ggaggcgtgt cgttttagtg
      421 aggtcactat cagcaaccat tattgcgatg agctaagcac atacgtcagc tatataaggt
      481 tttgctattc attgaaagca gtagtgactg atttgtatat atactcgaga ccctgcagtc
      541 cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac
      601 ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      661 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      721 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      781 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      841 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      901 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      961 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1021 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1081 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1141 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1201 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1261 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1321 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1381 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1441 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1501 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg
     1561 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     1621 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac
     1681 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
     1741 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     1801 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
     1861 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
     1921 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     1981 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     2041 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     2101 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     2161 aaggccggat aaaacttgtg ct
//
