LOCUS       sequence-299433-        2182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0456
FEATURES             Location/Qualifiers
     source          1..2182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     118..136
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(140..183)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      341..398
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(422..439)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(593..1181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(673..692)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1364..1458)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1479..2138)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2053..2072
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca
       61 actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
      121 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
      181 ttctggaatt cggtctcagg agggaggcgt gtcgttttag tgaggaacca ttattgcgat
      241 gagctaagca catacgtcag tcactatcag cctatataag gttttgctat tcattgaaag
      301 cagtagtgac tgatttgtat atatactcga gaccctgcag tccggcaaaa aagggcaagg
      361 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt
      421 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      481 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      541 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca
      601 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      661 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      721 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      781 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      841 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      901 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      961 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1021 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1081 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1141 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1201 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1261 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1321 aaagtatata tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa
     1381 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     1441 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
     1501 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     1561 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     1621 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     1681 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
     1741 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     1801 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     1861 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     1921 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     1981 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     2041 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     2101 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     2161 aatctcgata actcaaaaaa ta
//
