LOCUS       sequence-299438-        2339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0407
FEATURES             Location/Qualifiers
     source          1..2339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(147..241)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(262..921)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     836..855
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1083..1101
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1105..1148)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1463..1520
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1544..1561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1715..2303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1795..1814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
       61 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
      121 acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
      181 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
      241 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa
      301 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
      361 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
      421 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
      481 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
      541 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
      601 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
      661 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
      721 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
      781 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
      841 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
      901 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
      961 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc
     1021 gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     1081 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
     1141 atgatttctg gaattcggtc tcaggaggga ggcgtgtcgt tttagtgagg cagccacttg
     1201 tgttctgcac ctcacatgta ggctatcagc tagcaagtgg ataggttgtc tgcacctcac
     1261 atgttctctc tgccgacagt ggtcccaaaa aactggtact tgtgtacagg gctcaaacca
     1321 ttattgcgtc gcggtaggtc acgaccacta tgcccaaaaa tgtcaaagat aatcagctta
     1381 gcaagacctc tacctatata aggttttgct attcattgaa agcagtagtg actgatttgt
     1441 atatatactc gagaccctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt
     1501 tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc
     1561 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1621 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1681 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg
     1741 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1801 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1861 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1921 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1981 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2041 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2101 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2161 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2221 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2281 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgct
//
