LOCUS       sequence-299439-        2273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0408
FEATURES             Location/Qualifiers
     source          1..2273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     147..165
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(169..212)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      461..518
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(542..559)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(713..1301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(793..812)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1484..1578)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1599..2258)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2173..2192
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
       61 aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca
      121 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
      181 aaaaatcctt agctttcgct aaggatgatt tctggaattc ggtctcagga gggaggcgtg
      241 tcgttttagt gagggctttg tcaaaagcta aaaaagatga tgcctaggag gaccgatgct
      301 gatcttgcct gctctctctg ccgacagtgg tcccaaatag gtcacgacaa aaatgtcaaa
      361 gataccaatt aggttgtctg cacctcacat gctatataag gttttgctat tcattgaaag
      421 cagtagtgac tgatttgtat atatactcga gaccctgcag tccggcaaaa aagggcaagg
      481 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt
      541 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      601 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      661 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca
      721 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      781 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      841 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      901 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      961 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1021 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1081 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1141 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1201 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1261 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1321 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1381 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1441 aaagtatata tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa
     1501 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     1561 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
     1621 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     1681 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     1741 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     1801 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
     1861 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     1921 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     1981 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     2041 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     2101 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     2161 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     2221 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agc
//
