LOCUS       sequence-299443-        2395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0412
FEATURES             Location/Qualifiers
     source          1..2395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(12..106)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(127..786)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     701..720
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     948..966
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(970..1013)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1384..1441
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1465..1482)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1636..2224)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1716..1735)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
       61 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
      121 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
      181 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
      241 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
      301 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
      361 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
      421 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
      481 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
      541 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
      601 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
      661 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
      721 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
      781 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
      841 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc
      901 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
      961 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt
     1021 cggtctcagg agggaggcgt gtcgttttag tgaggatgag ctaagcacat acgtcagccg
     1081 atgctgatct tgcctgcctt gaaatttcgg gaaacctcct cgaaaactgg tacttgtgta
     1141 cagggctcac tctctctgcc gacagtggtc ccaaaagggc tcactgctag gaggaccgat
     1201 ggctttgtca aaagctaaaa aagatgatgc tgctgatctt tccatcaaca aataatccaa
     1261 gtaagcctca catgtctgac gtaagggatg acgcacatgc tgatcttgcc tgccctatat
     1321 aaggttttgc tattcattga aagcagtagt gactgatttg tatatatact cgagaccctg
     1381 cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
     1441 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1501 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1561 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1621 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg
     1681 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1741 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1801 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1861 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1921 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1981 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2041 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2101 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2161 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2221 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2281 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2341 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagc
//
