LOCUS       sequence-299444-        2312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0413
FEATURES             Location/Qualifiers
     source          1..2312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(21..115)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(136..795)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     710..729
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     957..975
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(979..1022)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1310..1367
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1391..1408)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1562..2150)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1642..1661)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgacagct cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
       61 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
      121 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
      181 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
      241 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
      301 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
      361 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
      421 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
      481 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
      541 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
      601 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
      661 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
      721 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
      781 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
      841 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa
      901 ctcgagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
      961 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
     1021 tctggaattc ggtctcagga gggaggcgtg tcgttttagt gaggtcagaa gatcaaaggg
     1081 ctagctagga ggaatgacgt aagccatgac gtctaccgat gctgatcttg ccaaaaatgt
     1141 caaagatacg actgttatgc ccaattaggt tgcaaatatt tcttgtgacc actatgccca
     1201 attaggttct gacgtaaggg atgacgcaca ggaggaccga ctatataagg ttttgctatt
     1261 cattgaaagc agtagtgact gatttgtata tatactcgag accctgcagt ccggcaaaaa
     1321 agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta
     1381 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1441 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1501 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1561 ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1621 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1681 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1741 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1801 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1861 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1921 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1981 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2041 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2101 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2161 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2221 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2281 aatcaatcta aagtatatat gagtaaactt gg
//
