LOCUS       sequence-299449-        2208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0418
FEATURES             Location/Qualifiers
     source          1..2208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     107..126
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     354..372
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(376..419)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      603..660
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(684..701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(855..1443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(935..954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1626..1720)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1741..2208,1..192))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
       61 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
      121 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
      181 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
      241 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc
      301 gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
      361 gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct
      421 ggaattcggt ctcaggaggg aggcgtgtcg ttttagtgag gcagtggtcc ctccacaggg
      481 ctcttttcaa caagacctct aaaccattat tgcggctatc agcttagcaa gacctatata
      541 aggttttgct attcattgaa agcagtagtg actgatttgt atatatactc gagaccctgc
      601 agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga
      661 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      721 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      781 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      841 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc
      901 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      961 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1021 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1081 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1141 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1201 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1261 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1321 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1381 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1441 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1501 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1561 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg
     1621 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
     1681 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
     1741 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     1801 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     1861 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     1921 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     1981 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     2041 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     2101 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     2161 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcg
//
