LOCUS       sequence-299451-        2308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0420
FEATURES             Location/Qualifiers
     source          1..2308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     255..274
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     502..520
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(524..567)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      851..908
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(932..949)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1103..1691)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1183..1202)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1874..1968)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1989..2308,1..340))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
       61 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
      121 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
      181 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
      241 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
      301 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
      361 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
      421 tggaacctct tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt
      481 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
      541 tccttagctt tcgctaagga tgatttctgg aattcggtct caggagggag gcgtgtcgtt
      601 ttagtgagga tgagctaagc acatacgtca gcttgccttt tcaacaaaac tgaagcatct
      661 tccggtcacg accactaatt gcgataaagg aaaggccaat taggttgtct ttatgacccc
      721 cgccgatgac gcgggacagc aagtggattt gcctgccttc agaagatcaa agggctacct
      781 cctatataag gttttgctat tcattgaaag cagtagtgac tgatttgtat atatactcga
      841 gaccctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac
      901 cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg
      961 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     1021 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1081 aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa
     1141 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1201 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1261 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1321 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1381 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1441 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1501 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1561 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1621 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1681 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1741 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1801 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1861 gctcgaggct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
     1921 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
     1981 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     2041 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     2101 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     2161 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     2221 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     2281 tcttgcgaat atatgtgtag aaactgcc
//
