LOCUS       sequence-299453-        2327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0422
FEATURES             Location/Qualifiers
     source          1..2327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     173..192
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     420..438
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(442..485)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      788..845
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(869..886)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1040..1628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1120..1139)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1811..1905)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1926..2327,1..258))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 atcccatatc accagctcac cgtctttcat tgccatacga aattccggat gagcattcat
       61 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
      121 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
      181 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
      241 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
      301 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat
      361 caactcgagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
      421 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
      481 atttctggaa ttcggtctca ggagggaggc gtgtcgtttt agtgaggtca gaagatcaaa
      541 gggctacttg cctgccttgc acaccagcat gtgttgatca ccagctaatg agctaagcac
      601 atacgtcagc acctcacatg taggctatca ggtggctcct actagcaaca gccacttgtg
      661 tgtcacgaca accacgtcta caaaattagg ttgtcagcaa gtggatctga tcttgcctct
      721 atataaggtt ttgctattca ttgaaagcag tagtgactga tttgtatata tactcgagac
      781 cctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
      841 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
      901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1021 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc
     1081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct
     1801 cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     1861 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     1921 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     1981 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     2041 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     2101 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     2161 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     2221 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     2281 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacact
//
