LOCUS       sequence-299455-        2202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0424
FEATURES             Location/Qualifiers
     source          1..2202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     158..177
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     405..423
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(427..470)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      648..705
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(729..746)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(900..1488)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(980..999)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1671..1765)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1786..2202,1..243))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
       61 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
      121 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
      181 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
      241 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
      301 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac
      361 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
      421 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgg
      481 tctcaggagg gaggcgtgtc gttttagtga ggaaccatta ttgcggctta gcaagacatg
      541 acgtaagcca tgacgtctac acatgtaggc tatcagctct atataaggtt ttgctattca
      601 ttgaaagcag tagtgactga tttgtatata tactcgagac cctgcagtcc ggcaaaaaag
      661 ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg
      721 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      781 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      841 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      901 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      961 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1021 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1081 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1141 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1201 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1261 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1321 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1381 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1441 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1501 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1561 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1621 tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca
     1681 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     1741 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     1801 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     1861 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     1921 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     1981 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     2041 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     2101 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     2161 gaaaacggtg taacaagggt gaacactatc ccatatcacc ag
//
