LOCUS       sequence-299457-        2183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0426
FEATURES             Location/Qualifiers
     source          1..2183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     121..140
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     368..386
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(390..433)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      592..649
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(673..690)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(844..1432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(924..943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1615..1709)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1730..2183,1..206))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
       61 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
      121 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
      181 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
      241 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
      301 tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
      361 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
      421 taaggatgat ttctggaatt cggtctcagg agggaggcgt gtcgttttag tgaggatgac
      481 gtaagccatg acgtctaaaa tttcgggaaa cctcctcggc aactatataa ggttttgcta
      541 ttcattgaaa gcagtagtga ctgatttgta tatatactcg agaccctgca gtccggcaaa
      601 aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt
      661 tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      721 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      781 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      841 gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      901 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      961 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1021 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1081 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1141 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1201 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1261 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1321 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1381 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1441 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1501 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1561 taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc
     1621 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     1681 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
     1741 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     1801 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     1861 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     1921 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
     1981 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     2041 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     2101 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     2161 ttgccatacg aaattccgga tga
//
