LOCUS       sequence-299458-        2394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0427
FEATURES             Location/Qualifiers
     source          1..2394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     341..360
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     588..606
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(610..653)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1023..1080
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1104..1121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1275..1863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1355..1374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2046..2140)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2161..2394,1..426))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
       61 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
      121 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
      181 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
      241 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
      301 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
      361 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
      421 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
      481 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc
      541 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
      601 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt
      661 cggtctcagg agggaggcgt gtcgttttag tgaggattgc gataaaggaa aggctggtgg
      721 aaaaagaaga ggtttgtgtg cacaccagca tgtgttgatc accagctaaa ctggtactta
      781 ggtggctcct actgtaggct atcaatcctt accgctatgg gtaagattgg accgatgctg
      841 ttatgacccc cgccgatgac gcgggatgtc tgcacctctg gtggagcacg acactctaca
      901 aaactggcag ccacttgtgt gcacctctct ctctgccgac agtggtccca aagctatata
      961 aggttttgct attcattgaa agcagtagtg actgatttgt atatatactc gagaccctgc
     1021 agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga
     1081 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1141 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1201 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1261 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1441 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1681 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1921 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1981 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg
     2041 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
     2101 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
     2161 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     2221 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     2281 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     2341 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaa
//
