LOCUS       sequence-299467-        2472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0258
FEATURES             Location/Qualifiers
     source          1..2472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     269..288
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     516..534
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(538..581)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        663..1008
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     951..971
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      1029..1086
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1110..1127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1281..1869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1361..1380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2052..2146)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2167..2472,1..354))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
       61 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
      121 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
      181 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
      241 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
      301 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
      361 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
      421 attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct
      481 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt
      541 tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg gtctcaggag
      601 gtcaacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct
      661 attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct
      721 atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat
      781 tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga
      841 cccccaccca cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa
      901 gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg
      961 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg ctactcgaga
     1021 ccctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
     1081 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1141 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1201 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1261 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
     1321 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1381 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1441 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1501 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1561 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1621 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1681 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1741 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1801 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1861 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1921 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1981 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     2041 tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     2101 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     2161 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     2221 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     2281 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     2341 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     2401 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     2461 ttgcgaatat at
//
