LOCUS       sequence-299473-        2719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0170
FEATURES             Location/Qualifiers
     source          1..2719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     737..757
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        746..792
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(925..942)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1035..1623)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1115..1134)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1797..2591)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2592..2696)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatt cggtctcagg aggtcaacat ggtggagcac gacactctgg tctactccaa
      421 aaatgtcaaa gatacagtct cagaagatca aagggctatt gagacttttc aacaaaggat
      481 aatttcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac
      541 agtagaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat
      601 tcaagatctc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
      661 ggaaaaagaa gaggttccaa ccacgtctac aatgatgtga catctccact gacgtaaggg
      721 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
      781 atttggagag gacacgctac tcgagaccct ctgggcctca tgggccttcc tttcactgcc
      841 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa catggtcata gctgtttcct
      901 tgcgtattgg gcgctctccg cttcctcgct cactgactcg ctgcgctcgg tcgttcgggt
      961 aaagcctggg gtgcctaatg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1021 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1081 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1141 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1201 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1261 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1321 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1381 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1441 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1501 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1561 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1621 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1681 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1741 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttattag
     1801 aaaaattcat ccagcagacg ataaaacgca atacgctggc tatccggtgc cgcaatgcca
     1861 tacagcacca gaaaacgatc cgcccattcg ccgcccagtt cttccgcaat atcacgggtg
     1921 gccagcgcaa tatcctgata acgatccgcc acgcccagac ggccgcaatc aataaagccg
     1981 ctaaaacggc cattttccac cataatgttc ggcaggcacg catcaccatg ggtcaccacc
     2041 agatcttcgc catccggcat gctcgctttc agacgcgcaa acagctctgc cggtgccagg
     2101 ccctgatgtt cttcatccag atcatcctga tccaccaggc ccgcttccat acgggtacgc
     2161 gcacgttcaa tacgatgttt cgcctgatga tcaaacggac aggtcgccgg gtccagggta
     2221 tgcagacgac gcatggcatc cgccataatg ctcacttttt ctgccggcgc cagatggcta
     2281 gacagcagat cctgacccgg cacttcgccc agcagcagcc aatcacggcc cgcttcggtc
     2341 accacatcca gcaccgccgc acacggaaca ccggtggtgg ccagccagct cagacgcgcc
     2401 gcttcatcct gcagctcgtt cagcgcaccg ctcagatcgg ttttcacaaa cagcaccgga
     2461 cgaccctgcg cgctcagacg aaacaccgcc gcatcagagc agccaatggt ctgctgcgcc
     2521 caatcatagc caaacagacg ttccacccac gctgccgggc tacccgcatg caggccatcc
     2581 tgttcaatca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2641 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2701 tttccccgaa aagtgccac
//
