LOCUS       sequence-299474-        2716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0169
FEATURES             Location/Qualifiers
     source          1..2716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     734..754
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        743..789
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(922..939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1032..1620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1112..1131)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1794..2588)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2589..2693)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatt cggtctcagg aggtcaacat ggtggagcac gacactctgg tctactccaa
      421 aaatgtcaaa gatacagtct cagaagatca aagggctatt gagacttttc aacaaaggat
      481 aatttcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac
      541 agtagaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat
      601 tcaagatctc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
      661 ggaaaaagaa gaggttccag caagtggatt gatgtgacat ctccactgac gtaagggatg
      721 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      781 tggagaggac acgctactcg agaccctctg ggcctcatgg gccttccttt cactgcccgc
      841 tttccagtcg ggaaacctgt cgtgccagct gcattaacat ggtcatagct gtttccttgc
      901 gtattgggcg ctctccgctt cctcgctcac tgactcgctg cgctcggtcg ttcgggtaaa
      961 gcctggggtg cctaatgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1021 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1081 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1141 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1201 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1261 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1321 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1381 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1441 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1501 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1561 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1621 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1681 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1741 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttattagaaa
     1801 aattcatcca gcagacgata aaacgcaata cgctggctat ccggtgccgc aatgccatac
     1861 agcaccagaa aacgatccgc ccattcgccg cccagttctt ccgcaatatc acgggtggcc
     1921 agcgcaatat cctgataacg atccgccacg cccagacggc cgcaatcaat aaagccgcta
     1981 aaacggccat tttccaccat aatgttcggc aggcacgcat caccatgggt caccaccaga
     2041 tcttcgccat ccggcatgct cgctttcaga cgcgcaaaca gctctgccgg tgccaggccc
     2101 tgatgttctt catccagatc atcctgatcc accaggcccg cttccatacg ggtacgcgca
     2161 cgttcaatac gatgtttcgc ctgatgatca aacggacagg tcgccgggtc cagggtatgc
     2221 agacgacgca tggcatccgc cataatgctc actttttctg ccggcgccag atggctagac
     2281 agcagatcct gacccggcac ttcgcccagc agcagccaat cacggcccgc ttcggtcacc
     2341 acatccagca ccgccgcaca cggaacaccg gtggtggcca gccagctcag acgcgccgct
     2401 tcatcctgca gctcgttcag cgcaccgctc agatcggttt tcacaaacag caccggacga
     2461 ccctgcgcgc tcagacgaaa caccgccgca tcagagcagc caatggtctg ctgcgcccaa
     2521 tcatagccaa acagacgttc cacccacgct gccgggctac ccgcatgcag gccatcctgt
     2581 tcaatcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2641 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2701 ccccgaaaag tgccac
//
