LOCUS       sequence-299475-        2708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0171
FEATURES             Location/Qualifiers
     source          1..2708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     726..746
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        735..781
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(914..931)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1024..1612)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1104..1123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1786..2580)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2581..2685)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatt cggtctcagg aggtcaacat ggtggagcac gacactctgg tctactccaa
      421 aaatgtcaaa gatacagtct cagaagatca aagggctatt gagacttttc aacaaaggat
      481 aatttcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac
      541 agtagaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat
      601 tcaagatctc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
      661 ggaaaaagaa gaggttccaa ccacgtctac aaagcaagtg gattgatgtg acatctccaa
      721 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
      781 acacgctact cgagaccctc tgggcctcat gggccttcct ttcactgccc gctttccagt
      841 cgggaaacct gtcgtgccag ctgcattaac atggtcatag ctgtttcctt gcgtattggg
      901 cgctctccgc ttcctcgctc actgactcgc tgcgctcggt cgttcgggta aagcctgggg
      961 tgcctaatga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1021 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1081 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1141 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1201 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1261 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1321 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1381 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1441 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1501 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1561 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1621 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1681 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1741 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttattaga aaaattcatc
     1801 cagcagacga taaaacgcaa tacgctggct atccggtgcc gcaatgccat acagcaccag
     1861 aaaacgatcc gcccattcgc cgcccagttc ttccgcaata tcacgggtgg ccagcgcaat
     1921 atcctgataa cgatccgcca cgcccagacg gccgcaatca ataaagccgc taaaacggcc
     1981 attttccacc ataatgttcg gcaggcacgc atcaccatgg gtcaccacca gatcttcgcc
     2041 atccggcatg ctcgctttca gacgcgcaaa cagctctgcc ggtgccaggc cctgatgttc
     2101 ttcatccaga tcatcctgat ccaccaggcc cgcttccata cgggtacgcg cacgttcaat
     2161 acgatgtttc gcctgatgat caaacggaca ggtcgccggg tccagggtat gcagacgacg
     2221 catggcatcc gccataatgc tcactttttc tgccggcgcc agatggctag acagcagatc
     2281 ctgacccggc acttcgccca gcagcagcca atcacggccc gcttcggtca ccacatccag
     2341 caccgccgca cacggaacac cggtggtggc cagccagctc agacgcgccg cttcatcctg
     2401 cagctcgttc agcgcaccgc tcagatcggt tttcacaaac agcaccggac gaccctgcgc
     2461 gctcagacga aacaccgccg catcagagca gccaatggtc tgctgcgccc aatcatagcc
     2521 aaacagacgt tccacccacg ctgccgggct acccgcatgc aggccatcct gttcaatcat
     2581 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2641 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2701 agtgccac
//
