LOCUS       sequence-299476-        2733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0174
FEATURES             Location/Qualifiers
     source          1..2733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(939..956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1049..1637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1129..1148)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1811..2605)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2606..2710)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatt cggtctcagg agtggaagat tcagaaaatc tccatcaaca aataatccaa
      421 gtaaggatta atggattgat caacatcctt accgctatgg gtaagattga tgaaaagtca
      481 aaaacaaaaa tcaattatgc acaccagcat gtgttgatca ccagctattg tgggacacca
      541 atttcgtcca cagacatcaa catcttatcg tcctttgaag ataagataat aatgttgaag
      601 ataagagtgg gagccaccac taaaacattc cgacagccac ttgtgtgaag catgtgaagc
      661 cggtccctcc actaagaaaa ttagtgaagc atcttccagt ggtccctcca ctcacagctc
      721 aatcagtgag caacaggacg aaggaaatga cgtaagccat gacgtctaat cccacaagaa
      781 tttccttata taaggaacac aaatcagaag gtactcgaga ccctctgggc ctcatgggcc
      841 ttcctttcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaacatggt
      901 catagctgtt tccttgcgta ttgggcgctc tccgcttcct cgctcactga ctcgctgcgc
      961 tcggtcgttc gggtaaagcc tggggtgcct aatgagcaaa aggccagcaa aaggccagga
     1021 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1081 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1141 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1201 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1261 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1321 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1381 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1441 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1501 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1561 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1621 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1681 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1741 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1801 ctgacagtta ttagaaaaat tcatccagca gacgataaaa cgcaatacgc tggctatccg
     1861 gtgccgcaat gccatacagc accagaaaac gatccgccca ttcgccgccc agttcttccg
     1921 caatatcacg ggtggccagc gcaatatcct gataacgatc cgccacgccc agacggccgc
     1981 aatcaataaa gccgctaaaa cggccatttt ccaccataat gttcggcagg cacgcatcac
     2041 catgggtcac caccagatct tcgccatccg gcatgctcgc tttcagacgc gcaaacagct
     2101 ctgccggtgc caggccctga tgttcttcat ccagatcatc ctgatccacc aggcccgctt
     2161 ccatacgggt acgcgcacgt tcaatacgat gtttcgcctg atgatcaaac ggacaggtcg
     2221 ccgggtccag ggtatgcaga cgacgcatgg catccgccat aatgctcact ttttctgccg
     2281 gcgccagatg gctagacagc agatcctgac ccggcacttc gcccagcagc agccaatcac
     2341 ggcccgcttc ggtcaccaca tccagcaccg ccgcacacgg aacaccggtg gtggccagcc
     2401 agctcagacg cgccgcttca tcctgcagct cgttcagcgc accgctcaga tcggttttca
     2461 caaacagcac cggacgaccc tgcgcgctca gacgaaacac cgccgcatca gagcagccaa
     2521 tggtctgctg cgcccaatca tagccaaaca gacgttccac ccacgctgcc gggctacccg
     2581 catgcaggcc atcctgttca atcatactct tcctttttca atattattga agcatttatc
     2641 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2701 gggttccgcg cacatttccc cgaaaagtgc cac
//
