LOCUS       sequence-299480-        2569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0177
FEATURES             Location/Qualifiers
     source          1..2569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(775..792)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(885..1473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(965..984)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1647..2441)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2442..2546)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatt cggtctcagg agagcggaga attaagggag tcacgttatg acccccgccg
      421 atgacgcggg acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc
      481 actcagccgc gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg
      541 cgcgttcaaa agtcgcctaa ggtagcaaat atttcttgtc aaaaatgctc cactgacgtt
      601 ccataaattc ccctcggtat ccaattagag tctcatattc actctcatac tcgagaccct
      661 ctgggcctca tgggccttcc tttcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      721 gctgcattaa catggtcata gctgtttcct tgcgtattgg gcgctctccg cttcctcgct
      781 cactgactcg ctgcgctcgg tcgttcgggt aaagcctggg gtgcctaatg agcaaaaggc
      841 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      901 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      961 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1021 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1081 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1141 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1201 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1261 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1321 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1381 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1441 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1501 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1561 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1621 tatgagtaaa cttggtctga cagttattag aaaaattcat ccagcagacg ataaaacgca
     1681 atacgctggc tatccggtgc cgcaatgcca tacagcacca gaaaacgatc cgcccattcg
     1741 ccgcccagtt cttccgcaat atcacgggtg gccagcgcaa tatcctgata acgatccgcc
     1801 acgcccagac ggccgcaatc aataaagccg ctaaaacggc cattttccac cataatgttc
     1861 ggcaggcacg catcaccatg ggtcaccacc agatcttcgc catccggcat gctcgctttc
     1921 agacgcgcaa acagctctgc cggtgccagg ccctgatgtt cttcatccag atcatcctga
     1981 tccaccaggc ccgcttccat acgggtacgc gcacgttcaa tacgatgttt cgcctgatga
     2041 tcaaacggac aggtcgccgg gtccagggta tgcagacgac gcatggcatc cgccataatg
     2101 ctcacttttt ctgccggcgc cagatggcta gacagcagat cctgacccgg cacttcgccc
     2161 agcagcagcc aatcacggcc cgcttcggtc accacatcca gcaccgccgc acacggaaca
     2221 ccggtggtgg ccagccagct cagacgcgcc gcttcatcct gcagctcgtt cagcgcaccg
     2281 ctcagatcgg ttttcacaaa cagcaccgga cgaccctgcg cgctcagacg aaacaccgcc
     2341 gcatcagagc agccaatggt ctgctgcgcc caatcatagc caaacagacg ttccacccac
     2401 gctgccgggc tacccgcatg caggccatcc tgttcaatca tactcttcct ttttcaatat
     2461 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2521 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
