LOCUS       sequence-299484-        2510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0183
FEATURES             Location/Qualifiers
     source          1..2510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(716..733)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(826..1414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(906..925)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1588..2382)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2383..2487)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctctcggt ctcaggaggg aggcgtgtcg ttttagtgag
      421 gtgaagataa gataataatg ttgaagataa gagtgggagc caccagcttt gtcaaaagct
      481 aaaaaagatg atgcatgacg taagccatga cgtctactat ataaggtttt gctattcatt
      541 gaaagcagta gtgactgatt tgtatataat gtactcgaga ccctggtacc tggagcacaa
      601 gactggcctc atgggccttc ctttcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      661 agctgcatta acatggtcat agctgtttcc ttgcgtattg ggcgctctcc gcttcctcgc
      721 tcactgactc gctgcgctcg gtcgttcggg taaagcctgg ggtgcctaat gagcaaaagg
      781 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      841 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      901 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      961 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1021 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1081 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1141 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1201 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1261 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1321 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1381 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1441 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1501 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1561 atatgagtaa acttggtctg acagttatta gaaaaattca tccagcagac gataaaacgc
     1621 aatacgctgg ctatccggtg ccgcaatgcc atacagcacc agaaaacgat ccgcccattc
     1681 gccgcccagt tcttccgcaa tatcacgggt ggccagcgca atatcctgat aacgatccgc
     1741 cacgcccaga cggccgcaat caataaagcc gctaaaacgg ccattttcca ccataatgtt
     1801 cggcaggcac gcatcaccat gggtcaccac cagatcttcg ccatccggca tgctcgcttt
     1861 cagacgcgca aacagctctg ccggtgccag gccctgatgt tcttcatcca gatcatcctg
     1921 atccaccagg cccgcttcca tacgggtacg cgcacgttca atacgatgtt tcgcctgatg
     1981 atcaaacgga caggtcgccg ggtccagggt atgcagacga cgcatggcat ccgccataat
     2041 gctcactttt tctgccggcg ccagatggct agacagcaga tcctgacccg gcacttcgcc
     2101 cagcagcagc caatcacggc ccgcttcggt caccacatcc agcaccgccg cacacggaac
     2161 accggtggtg gccagccagc tcagacgcgc cgcttcatcc tgcagctcgt tcagcgcacc
     2221 gctcagatcg gttttcacaa acagcaccgg acgaccctgc gcgctcagac gaaacaccgc
     2281 cgcatcagag cagccaatgg tctgctgcgc ccaatcatag ccaaacagac gttccaccca
     2341 cgctgccggg ctacccgcat gcaggccatc ctgttcaatc atactcttcc tttttcaata
     2401 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2461 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
