LOCUS       sequence-299488-       10852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid level alpha effector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 10852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC-alpha-1KN0007
FEATURES             Location/Qualifiers
     source          1..10852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    17..41
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1342..1971
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIGTHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2405..3472
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3538..3732
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3968..3990)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4076..4216
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4131..4150
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4231..4248)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4402..4990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4482..4501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5078..5893)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5078..5893)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5801..5820
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    6326..6350
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    6663..6718
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      10138..10845
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 gtcatgagac gaattctgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
       61 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
      121 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct
      181 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc atctgaaaac gacatgtcgc
      241 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg
      301 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga
      361 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact
      421 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga
      481 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg
      541 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg
      601 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg
      661 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt
      721 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag
      781 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc
      841 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct
      901 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc
      961 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg
     1021 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat
     1081 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca
     1141 cctctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc
     1201 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt
     1261 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatccgat gagtaaataa
     1321 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca
     1381 ggcaagacga ccatcggaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt
     1441 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat
     1501 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc
     1561 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc
     1621 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
     1681 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
     1741 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
     1801 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
     1861 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
     1921 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
     1981 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
     2041 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
     2101 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
     2161 ccattaccga gctgctatct gaatagatcg cgcagctacc agagtaaatg agcaaatgaa
     2221 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
     2281 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
     2341 ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
     2401 tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt
     2461 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc
     2521 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc
     2581 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt
     2641 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt
     2701 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg
     2761 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt
     2821 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga
     2881 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc
     2941 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca
     3001 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg
     3061 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat
     3121 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt
     3181 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg
     3241 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga
     3301 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg
     3361 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct
     3421 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga
     3481 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt
     3541 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg
     3601 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa
     3661 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct
     3721 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct
     3781 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg
     3841 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc
     3901 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg
     3961 gtgaaaacct ctgacacatg cagctcccgg tgacggtcac agcttgtctg taagcggatg
     4021 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     4081 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     4141 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     4201 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4261 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4321 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4381 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4441 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4501 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4561 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4621 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4681 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4741 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4801 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     4861 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4921 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4981 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5041 ctcacgttaa gggattttgg tcatgcattc taggtgatta gaaaaactca tcgagcatca
     5101 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     5161 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     5221 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     5281 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     5341 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     5401 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgagt cgaaatacgc
     5461 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     5521 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     5581 ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     5641 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     5701 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     5761 catacaatcg gtagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     5821 catataaatc agcatccatg ttggaattta atcgcggcct tgagcaagac gtttcccgtt
     5881 gaatatggct cataacagaa cttattattt ccttcctctt ttctacagta tttaaagata
     5941 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac
     6001 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta
     6061 tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac
     6121 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
     6181 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
     6241 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
     6301 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac ataacgaatt
     6361 cgtctcagga gggaggcgtg tcgttttagt gaggagcaag tggatgtagg tcacgaccat
     6421 cactatcagc gatcttgcct gccttttatg acccccgccg atgacgcggg aaggaggacc
     6481 gatgctgatt ctctctgccg acagtggtcc caaaggttgt ctgcaccaac cattattgcg
     6541 tcacatgtag gctatcagct tatgacgtaa gccatgacgt ctagacctct acaaactata
     6601 taaggttttg ctattcattg aaagcagtag tgactgattt gtatatatac tgtattttta
     6661 caacaattac caacaacaac aaacaacaaa caacattaca attactattt acaattacaa
     6721 tggatcccat tcgcagtcgt acgcctagtc ctgctagaga gcttttacct ggaccacagc
     6781 cagatcgagt gcagcctaca gctgatcgtg ggggcgcgcc acccgctggt ggacctctcg
     6841 atgggttgcc ggctcgaagg acaatgtcca gaactcgttt accttcacct cctgccccat
     6901 cacctgcttt ctccgccggg tcttttaatg accttttgag gcaatttgat ccatcacttc
     6961 ttgatacaag tttgttagat agcatgccgg ctgtaggaac tccacacacc gcggcagctc
     7021 ctgctgaatg ggacgaagtg cagtccggat tgagagcagc tgatgatcct ccccctacag
     7081 ttagagttgc agttacggct gcaagacccc cacgcgcaaa gccagcgcca cgacgtagag
     7141 ccgctcagcc cagtgatgct agcccggcag cacaagttga tctcaggact cttggttatt
     7201 cacagcaaca gcaggaaaag attaaatcta aagtaaggtc cactgtggct caacatcatg
     7261 aggctctcgt aggtcacgga ttcactcatg ctcatatagt tgcattatct cagcatccag
     7321 ccgctctcgg taccgtggca gtgaagtacc agcatattat tacagctttg cctgaagcta
     7381 cgcatgagga tattgttgga gttggcaagc agtggagcgg tgccagagca ctcgaagcac
     7441 ttcttactaa agctggagag ttgagaggtc cgccactaca attggacacc gggcagttgc
     7501 ttaaaatcgc taagcggggg ggagttactg ccgttgaggc ggtacacgca tcgagaaatg
     7561 cattgactgg agcccccctg aaccttacac cagaccaagt ggtcgctatc gctagtaata
     7621 tcggaggcaa tcaggcgtta gagacagtgc aacgtctatt acccgtgcta tgccaggatc
     7681 atgggctcac tcccgatcag gtcgtcgcca tcgctaacaa caatggcggt aaacaagcat
     7741 tagaaaccgt ccaaaggttg ctgccagtcc tctgtcaggc tcacggcctt acacctgatc
     7801 aagttgtagc tattgcatct aacggaggca agcaggcact tgagactgtc cagaggcttc
     7861 tgccagtttt atgccaggct catggtctga cccctaatca ggtggttgcc atcgcgtcta
     7921 atggtggggg aaaacaagct ctcgaaactg ttcagaggtt attaccagtc ctttgtcaag
     7981 ctcatggcct tactcaagat caagtagttg cgatcgccag taactctgga ggtaagcaag
     8041 cccttgaaac cgtgcaaagg ctcctccctg tactctgcca ggcgcatggc ctcacgcctg
     8101 ctcaggtggt agctatcgca tctaacaatg gtggtaagca ggcattggaa acagtccaga
     8161 gactcttccc agtcctgtgc caagatcatg ggttgacccc cgatcaagtg gtgactatag
     8221 caaataacaa tggagggaag caggcgctgg agacagttca acgactcctt cctgttctct
     8281 gtcaagcaca cggtttgatt ccagatcagg tagtggccat tgccaacaat aacgggggca
     8341 agcaagcgtt agaaactgta caacggcttc ttccagtgct atgtcaagcc cacgggttga
     8401 ctccagcaca ggttgtcgct attgcttcaa acataggcgg aaagcaagca ctggaaaccg
     8461 tgcagcgact tctaccagta ctatgtcgag ctcacggact tacccctgct caagttgtcg
     8521 caattgccaa taataatggt ggcaaacagg ctcttgaaac agttcaaagg cttctccctg
     8581 tcctatgtca ggcacatggc ctgacacccg accaagtcgt tgccatagcc agcaatattg
     8641 gtgggaagca agcattggag actgtgcaac ggttattgcc tgtcctttgc caagatcatg
     8701 gtttgacgcc tgaccaagtt gtggcgattg cctctaatgg aggaaagcag gcccttgaga
     8761 cagtccagcg gctactccct gtcttatgcc aagaccatgg cttgacccct gcacaagtgg
     8821 ttgcgatagc atcacatgat gggggtaaac aggctttgga aacggttcag aggctactgc
     8881 cagtattgtg ccaggaccat ggcctaacac tggatcaagt tgttgctata gcgtcgcacg
     8941 atggaggcaa acaagcgttg gaaaccgttc aaagattgct gcctgttttg tgtcaagatc
     9001 acggtcttac actagatcag gttgtggcaa tcgcttctaa tatcgggggt aagcaggcct
     9061 tagagacagt acaaagatta ctaccagttc tgtgtcagga tcacggactg actcctgacc
     9121 aggtagttgc tatcgcttca aatggtggcg ggaaacaggc gcttgagaca gtacagaggt
     9181 tgcttccagt tctttgccag gatcatggac ttactccaga ccaggtggtg gctattgcta
     9241 gtaatggcgg tggaaaacag gccttggaat caattgttgc tcagttgtca agacctgatc
     9301 ctgccctggc tgccctaact aatgaccacc ttgtggcctt agcgtgtctt ggtgggcgtc
     9361 cggcattaga tgccgtaaaa aagggccttc cacacgcccc tgagttgata cgtagaatta
     9421 atcgtcgcat tcctgaacgc acctctcatc gtgtggcaga ccttgcgcat gttgttagag
     9481 ttttaggttt ctttcaaagt cactcccatc ctgcccaggc tttcgatgat gctatgaccc
     9541 aatttggaat gagtcggcat ggcttggtcc agctatttag aagggttggt gttactgagt
     9601 ttgaggctcg ttgtggcaca ctgccgccag cctcacaaag atgggacagg attttgcaag
     9661 cgtctggtat gaagcgtgca aagccatccc ctaccagcgc tcaaacaccg gatcaagcct
     9721 ctttacatgc ctttgctgac tcccttgaga gagatttaga tgctccatcc ccgatgcatg
     9781 aaggcgacca gactagagct tcatcgagga agagatcccg aagtgatcga gcagtaactg
     9841 gtccttcgac acagcagtct ttcgaggtta gagtcccaga acaacgcgat gctctacact
     9901 tacccttgtc ttggagagtc aaacgcccac gtactaggat aggaggcggt ttaccagatc
     9961 cgggaacacc tatcgcagca gacttggctg catctagtac tgttatgtgg gaacaggacg
    10021 ctgctccttt tgctggtgca gctgacgatt tccctgcttt taacgaagaa gaattggcat
    10081 ggctcatgga gctgcttcca caatcgggta gtgtcggggg tacgatctga gcttgtcctg
    10141 ctttaatgag atatgcgaga agcctatgat cgcatgatat ttgctttcaa ttctgttgtg
    10201 cacgttgtaa aaaacctgag catgtgtagc tcagatcctt accgccggtt tcggttcatt
    10261 ctaatgaata tatcacccgt tactatcgta tttttatgaa taatattctc cgttcaattt
    10321 actgattgta ccctactact tatatgtaca atattaaaat gaaaacaata tattgtgctg
    10381 aataggttta tagcgacatc tatgatagag cgccacaata acaaacaatt gcgttttatt
    10441 attacaaatc caattttaaa aaaagcggca gaaccggtca aacctaaaag actgattaca
    10501 taaatcttat tcaaatttca aaagtgcccc aggggctagt atctacgaca caccgagcgg
    10561 cgaactaata acgctcactg aagggaactc cggttccccg ccggcgcgca tgggtgagat
    10621 tccttgaagt tgagtattgg ccgtccgctc taccgaaagt tacgggcacc attcaacccg
    10681 gtccagcacg gcggccgggt aaccgacttg ctgccccgag aattatgcag catttttttg
    10741 gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga cagtgacgac aaatcgttgg
    10801 gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc agcaggaccg ct
//
