LOCUS       sequence-299509-        8986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-GIGYF1 in mammalian cells, can be used to make 
            inducible cell line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Tollenaere MAX, Tiedje C, Rasmussen S, Nielsen JC, Vind AC, Blasius 
            M, Batth TS, Mailand N, Olsen JV, Gaestel M, Bekker-Jensen S
  TITLE     GIGYF1/2-Driven Cooperation between ZNF598 and TTP in 
            Posttranscriptional Regulation of Inflammatory Signaling.
  JOURNAL   Cell Rep. 2019 Mar 26;26(13):3511-3521.e4. doi: 
            10.1016/j.celrep.2019.03.006.
  PUBMED    30917308
REFERENCE   2  (bases 1 to 8986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.03"; journalName: "Cell Rep"; date: 
            "2019-03-26- 26"; volume: "26"; issue: "13"; pages: "3511-3521.e4"
COMMENT     Sequence Label: pcDNA4/TO/GFP-GIGYF1
FEATURES             Location/Qualifiers
     source          1..8986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(984..986,987..989,990..1697)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             join(984..986,987..989,990..1697)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(1029..1050)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1290..1309)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1637..1658
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1716..1740
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4876..4900)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4994..5011)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5000..5224
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5270..5698
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5357..5376)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5567..5588
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5707..5727)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5712..6041
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5892..6027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5954..5973
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6089..6136
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6155..6529
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6659..6780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6696..6715)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6750..6769
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6829..6845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6829..6845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6842..6864)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6853..6869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6877..6883,6884..6901,6902..6907))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6922..6943
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7060..7077)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7231..7819)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7311..7330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7990..8781,8782..8850))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8613..8632
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8851..8955)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttatggtga gcaagggcga ggagctgttc accggggtgg
     1021 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1081 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1141 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     1201 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1261 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1321 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1381 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     1441 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     1501 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     1561 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     1621 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     1681 gcatggacga gctgtacggt accgagctcg gatctacaag tttgtacaaa aaagcagaag
     1741 ggccgtcaag gcccaccatg gcagcagaga cactcaactt tgggcctgag tggctcaggg
     1801 ccctgtccgg gggcggcagc gtggcctccc cacccccgtc ccctgccatg cccaaataca
     1861 agctggctga ctaccgttat gggcgagagg aaatgctggc tctctacgtc aaggagaaca
     1921 aggtcccgga agagctgcag gacaaggagt tcgccgcggt gctgcaggac gagccactgc
     1981 agcccctggc tctggagccg ctgactgagg aggaacagag aaacttctcc ctgtcagtga
     2041 acagcgtggc tgtgctgagg ctgatgggga aaggggctgg cccccccctg gctggcacct
     2101 cccgaggcag gggcagcacg cggagccgag gccgcggccg tggtgacagc tgcttttacc
     2161 aaagaagcat cgaagaaggc gatggggcct ttggacgaag cccccgggaa atccagcgca
     2221 gccagagctg ggatgacaga ggcgagaggc ggtttgagaa gtcagcaagg cgggatggag
     2281 cacgatgtgg ctttgaggag ggaggggctg gcccaaggaa ggagcacgcc cgctcagaca
     2341 gcgagaactg gcgctcccta cgggaggaac aggaggagga ggaggagggc agctggaggc
     2401 tcggagcagg gccccggcga gacggcgacc gctggcgctc cgccagccct gatggtggtc
     2461 cccgctctgc tggctggcgg gaacatgggg aacggcggcg caagtttgaa tttgatttgc
     2521 gaggggatcg aggagggtgt ggtgaagagg aggggcgggg agggggaggc agctctcacc
     2581 tgcggcggtg ccgagcgcct gaaggctttg aggaggacaa ggatgggctc ccagagtggt
     2641 gcctggacga tgaggatgaa gaaatgggca cctttgatgc ctctggggcc ttcttgcctc
     2701 tcaagaaggg ccccaaggag cccattcctg aggagcagga gctggacttc caagggttgg
     2761 aggaggagga ggaaccttcc gaagggctag aggaggaagg gcctgaggca ggtgggaaag
     2821 agctgacccc actgcctcct caggaggaga agtccagctc cccatcccca ctgcccaccc
     2881 tgggcccact ctgggggaca aacggggatg gggacgaaac tgcagagaaa gagcccccag
     2941 cggccgaaga tgatattcgg gggatccagc tgagtcccgg ggtgggctcc tctgctggcc
     3001 cacccggaga tctggaggat gatgaaggct tgaagcacct gcagcaggag gcggagaagc
     3061 tggtggcctc cctgcaggac agctccttgg aggaggagca gttcacggct gccatgcaga
     3121 cccagggcct gcgccactct gcagccgcca ctgccctccc gctcagccat ggggctgccc
     3181 ggaagtggtt ctacaaggac ccacagggcg agatccaagg ccccttcacg acacaggaga
     3241 tggcagagtg gttccaggcc ggctactttt ccatgtcact gctggtgaag cggggctgcg
     3301 atgagggctt ccagccgctg ggcgaggtga tcaagatgtg gggccgcgtg ccctttgccc
     3361 cagggccctc acctccccca ctgctgggaa acatggacca ggagcggctg aagaagcaac
     3421 aggagctggc cgcggcggcc ttgtaccagc agctgcagca ccagcagttt ctccagctgg
     3481 tcagcagccg ccagctcccg cagtgcgcgc tccgagaaaa ggcagctctg ggggacctga
     3541 caccgccacc accgccgccg ccacagcagc agcagcagca gctcacggca ttcctgcagc
     3601 agctccaggc gctcaaaccc cccagaggcg gggaccagaa cctgctcccg acgatgagcc
     3661 ggtccttgtc ggtgccagat tcgggccgcc tctgggacgt acatacctca gcctcatcac
     3721 agtcaggtgg tgaggccagt ctttgggaca taccaattaa ctcttcgact cagggtccaa
     3781 ttctagaaca actccagctg caacataaat tccaggagcg cagagaagtg gagctcaggg
     3841 cgaagcggga ggaagaggaa cgcaagcgtc gagaggagaa gcgccgccag cagcagcagg
     3901 aggagcagaa gcggcggcag gaggaggaag agctgtttcg gcgcaagcac gtgcggcagc
     3961 aggagctatt gctgaagttg ctacagcagc agcaggcggt ccctgtgccc cccgcaccca
     4021 gctccccgcc cccactctgg gctggcctgg ccaagcaggg gctgtccatg aagacgctcc
     4081 tggagttgca gctggagggc gagcggcagc tgcacaaaca gcccccacct cgggagccag
     4141 ctcgggccca ggcccccaac caccgagtgc agcttggggg cctgggcact gcccccctga
     4201 accagtgggt gtctgaggct gggccactgt ggggcgggcc agacaagagt gggggcggca
     4261 gcagcggcct ggggctctgg gaggacaccc ccaagagcgg cgggagcctg gtccgtggcc
     4321 tcggcctgaa gaacagccgg agcagcccat ctctcagtga ctcatacagc cacctatcgg
     4381 gtcggcccat tcgcaaaaag acggaggaag aagagaagct gctgaagctg ctgcagggca
     4441 ttcccaggcc ccaggacggc ttcacccagt ggtgcgagca gatgctgcac acgctgagcg
     4501 ccacgggcag cctggacgtg cccatggctg tagcgatcct caaggaggtg gaatccccct
     4561 atgatgtcca cgattatatc cgttcctgcc tgggggacac gctggaagcc aaagaatttg
     4621 ccaaacaatt cctggagcgg agggccaagc agaaagccag ccagcagcgg cagcagcagc
     4681 aggaggcatg gctgagcagc gcctcgctgc agacggcctt ccaggccaac cacagcacca
     4741 aactcggccc cggggagggc agcaaggcca agaggcgggc actgatgctg cactcagacc
     4801 ccagcatcct ggggtactcc ctgcacggat cttctggtga gatcgagagc gtggatgact
     4861 actagggcct catgggccca gctttcttgt acaaagtggt agatccacta gtccagtgtg
     4921 gtggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa
     4981 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5041 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     5101 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5161 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     5221 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     5281 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5341 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5401 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5461 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5521 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5581 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5641 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     5701 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5761 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5821 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     5881 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5941 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     6001 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     6061 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     6121 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     6181 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     6241 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     6301 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     6361 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     6421 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     6481 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
     6541 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     6601 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     6661 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6721 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6781 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     6841 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6901 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6961 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7021 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7081 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7141 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7201 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7261 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7321 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7381 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7441 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7501 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7561 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7621 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7681 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7741 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7801 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7861 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7921 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7981 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8041 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8101 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8161 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8221 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8281 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8341 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8401 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8461 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8521 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8581 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8641 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8701 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8761 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8821 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8881 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8941 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
