LOCUS       sequence-299547-        8460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-ZNF598 mutated in polyproline streches in mammalian 
            cells, can be used to make inducible cell line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8460)
  AUTHORS   Tollenaere MAX, Tiedje C, Rasmussen S, Nielsen JC, Vind AC, Blasius 
            M, Batth TS, Mailand N, Olsen JV, Gaestel M, Bekker-Jensen S
  TITLE     GIGYF1/2-Driven Cooperation between ZNF598 and TTP in 
            Posttranscriptional Regulation of Inflammatory Signaling.
  JOURNAL   Cell Rep. 2019 Mar 26;26(13):3511-3521.e4. doi: 
            10.1016/j.celrep.2019.03.006.
  PUBMED    30917308
REFERENCE   2  (bases 1 to 8460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.03"; journalName: "Cell Rep"; date: 
            "2019-03-26- 26"; volume: "26"; issue: "13"; pages: "3511-3521.e4"
COMMENT     Sequence Label: pcDNA4/TO/GFPZNF598-9P-9A
FEATURES             Location/Qualifiers
     source          1..8460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(990..992,993..995,996..1706)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(990..992,993..995,996..1706)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1035..1056)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1643..1664
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4468..4485)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4474..4698
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4744..5172
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4831..4850)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5041..5062
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5181..5201)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5186..5515
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5366..5501
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5428..5447
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5563..5610
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5629..6003
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6133..6254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6170..6189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6224..6243
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6303..6319)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6303..6319)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6316..6338)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6327..6343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6351..6357,6358..6375,6376..6381))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6396..6417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6534..6551)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6705..7293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6785..6804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7464..8255,8256..8324))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8087..8106
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8325..8429)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacca tggtgagcaa gggcgaggag ctgttcaccg
     1021 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1081 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1141 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     1201 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1261 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1321 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1381 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1441 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1501 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1561 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     1621 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1681 ctctcggcat ggacgagctg tacaagagaa ttcaaatggc ggcggcgggg ggcgccgagg
     1741 ggcggcgcgc ggccctggag gcggcggcgg cggcagctcc tgagcgggga ggcgggagct
     1801 gcgtgctgtg ctgcggagac ctggaggcca cggcgctggg ccgctgcgac cacccggtgt
     1861 gctaccgctg ctctaccaag atgcgggtgc tctgcgagca gcgctactgc gccgtgtgcc
     1921 gcgaggagct gcgccaggtg gtctttggga agaagcttcc tgcctttgcc accatcccca
     1981 tccaccagct gcagcatgag aagaaatatg atatctactt tgcagatgga aaggtgtacg
     2041 cattgtacag gcagctgctg cagcacgagt gcccgcggtg ccccgagctg ccacctttca
     2101 gcctcttcgg ggacctggag cagcacatgc ggaggcagca tgagctcttc tgctgccgtc
     2161 tgtgcctcca gcacctccag atcttcacat atgagcgcaa gtggtactcg cgcaaggacc
     2221 tggcccggca tcgcatgcag ggtgaccccg atgacacgtc gcaccgtggg cacccgctct
     2281 gcaagttctg tgacgagcgc tacctggaca atgatgagct gcttaagcac ctgcgccgcg
     2341 accactactt ctgccacttc tgcgactcgg acggggccca ggactactac agcgactatg
     2401 cctacctgcg tgagcacttc cgggagaagc actttctgtg tgaggaaggc cgctgcagca
     2461 cggagcagtt cacccacgcc ttccgcaccg agatcgacct caaggcccac aggacggcct
     2521 gccacagtcg cagccgcgcc gaggcacgcc agaaccgcca catcgacctg cagttcagct
     2581 acgcgccacg gcactcgcgc cggaacgagg gggtcgttgg tggcgaagac tacgaggagg
     2641 tggacaggta cagccgccag ggccgagtgg cccgggctgg cactcgcgga gcccagcaga
     2701 gccgccgagg aagctggagg tacaaaaggg aagaagagga ccgagaagta gcagctgctg
     2761 tccgggcctc cgtggccgca cagcagcagg aggaggctcg caggagtgag gatcaggagg
     2821 aaggcggtag gcccaagaag gaggaggcag cggcgcgggg acctgaggat ccccgtggcc
     2881 cccggcgctc accccggact cagggcgaag gcccaggccc caaggaaacc tcgacaaatg
     2941 gtcctgtaag ccaagaagcc ttctcggtga caggcccagc cgccccaggg tgtgtggggg
     3001 tgccaggcgc cctcccacca cccagcccga agctcaagga cgaagacttc cccagcctct
     3061 ctgcctccac ttcctcctcc tgctccactg cagcaacccc gggccctgtg gggttggcgc
     3121 tgccgtacgc catccctgcc agaggcagga gtgccttcca ggaggaggac ttccccgccc
     3181 tggtgtcctc ggtgcccaag cctggcaccg cccccaccag ccttgtctct gcctggaaca
     3241 gcagcagtag cagcaagaag gtagcacagc ccccactctc ggcgcaggct accggcagcg
     3301 gccagcccac caggaaggct gggaagggga gcaggggcgg caggaagggc ggcccgccct
     3361 tcacacagga ggaggaggag gacggcggcc cggccctgca ggagcttctg agcacacgcc
     3421 ccacgggctc cgtctcctcc acactggggc tggcctccat ccaaccctct aaagttggga
     3481 agaagaagaa agtgggctcg gagaagccag gcaccacatt gccacagccc ccgcccgcta
     3541 cctgtccccc aggggctttg caggccccgg aagctcctgc cagcagagcc gaggggccag
     3601 ttgccgtcgt cgttaatgga cacatggagg gcccggcccc tgctcggagt gcccccaagg
     3661 aaccccctgg gctcccaagg cccctggggt ccttcccctg ccccacgcca caggaggact
     3721 tcccagcgct cggcggcccc tgcccacccc ggatgccggc ggccgcaggc ttcagcgctg
     3781 tggtgctcct gaagggcacg cctcccccag ccgcggcggg cctggtgccc ccaatcagca
     3841 aggcggccgc tggcttctct ggccttctgc ctagccccca cccggcctgt gtccccagcc
     3901 ccgccaccac caccaccaca aaagcaccca ggctgctgcc tgccccacgg gcgtacctag
     3961 tccccgagaa cttccgggag aggaaccttc agctcatcca gtccatcagg gacttcctgc
     4021 agagcgacga ggcccgcttc agcgagttca agagccactc aggggagttc agacagggcc
     4081 tgatctccgc agcccagtat tacaagagtt gccgggacct gctgggggag aatttccaga
     4141 aggtctttaa tgagctgctg gtcctgctgc ccgacacggc caagcagcag gagctcctgt
     4201 ctgcacacac ggacttctgc aaccgcgaga agcctctgag caccaagtcc aagaagaaca
     4261 agaagagcgc gtggcaggcc accacccagc aggcgggcct ggactgccgt gtgtgcccca
     4321 cctgccagca ggtgctcgcg catggcgatg ccagcagcca ccaggcgctg catgctgccc
     4381 gggacgacga cttcccctcc ctgcaagcca tcgccaggat catcacgtag ctcgagtcta
     4441 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     4501 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     4561 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     4621 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     4681 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     4741 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4801 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4861 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4921 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4981 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5041 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5101 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5161 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     5221 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5281 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5341 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5401 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     5461 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     5521 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     5581 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     5641 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     5701 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     5761 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     5821 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     5881 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     5941 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     6001 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6061 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6121 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6181 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6241 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6301 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6361 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6421 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6481 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7141 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7741 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7801 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7861 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7921 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7981 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8041 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8101 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8161 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8221 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8281 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8341 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
