LOCUS       sequence-299558-        5341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Flag tagged human FOXQ1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Moparthi L, Koch S
  TITLE     A uniform expression library for the exploration of FOX 
            transcription factor biology.
  JOURNAL   Differentiation. 2020 Sep - Oct;115:30-36. doi: 
            10.1016/j.diff.2020.08.002. Epub 2020 Aug 20.
  PUBMED    32858261
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Differentiation. 2020 Sep - Oct"; volume: "115"; pages: "30-36. 
            doi: 10.1016/j.diff.2020.08.002. Epub 2020 Aug 20"
COMMENT     Sequence Label: Flag-FOXQ1
FEATURES             Location/Qualifiers
     source          1..5341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    1393..1527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1417..1436)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1471..1490
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1640..1660)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1640..1658)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1679..1695)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1679..1695)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1692..1714)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1703..1719
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1727..1733,1734..1751,1752..1757))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1772..1793
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1910..1927)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2081..2669)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2161..2180)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2840..3631,3632..3700))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3463..3482
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3701..3805)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3831..4286)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3968..3989)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4180..4199
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4357..5341
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcagccacc atggattaca aggacgacga cgataaggga
      121 tcccatcgat tcgaattcaa gttggaggtg ttcgtccctc gcgcggccca cggggacaag
      181 cagggcagtg acctggaggg cgcgggcggc agcgacgcgc cgtccccgct gtcggcggcg
      241 ggagacgact ccctgggctc agatggggac tgcgcggcca acagcccggc cgcgggcggc
      301 ggcgccagag atccgccggg cgacggcgaa cagagtgcgg gaggcgggcc gggcgcggag
      361 gaggcgatcc cggcagcagc tgctgcagcg gtggtggcgg agggcgcgga ggccggggcg
      421 gcggggccag gcgcgggcgg cgcggggagc ggcgagggtg cacgcagcaa gccatatacg
      481 cggcggccca agccccccta ctcgtacatc gcgctcatcg ccatggccat ccgcgactcg
      541 gcgggcgggc gcttgacgct ggcggagatc aacgagtacc tcatgggcaa gttccccttt
      601 ttccgcggca gctacacggg ctggcgcaac tccgtgcgcc acaacctttc gctcaacgac
      661 tgcttcgtca aggtgctgcg cgacccctcg cggccctggg gcaaggacaa ctactggatg
      721 ctcaacccca acagcgagta caccttcgcc gacggggtct tccgccgccg ccgcaagcgc
      781 ctcagccacc gcgcgccggt ccccgcgccc gggctgcggc ccgaggaggc cccgggcctc
      841 cccgccgccc cgccgcccgc gcccgccgcc ccggcctcgc cccgcatgcg ctcgcccgcc
      901 cgccaggagg agcgcgccag ccccgcgggc aagttctcca gctccttcgc catcgacagc
      961 atcctgcgca agcccttccg cagccgccgc ctcagggaca cggcccccgg gacgacgctt
     1021 cagtggggcg ccgcgccctg cccgccgctg cccgcgttcc ccgcgctcct ccccgcggcg
     1081 ccctgcaggg ccctgctgcc gctctgcgcg tacggcgcgg gcgagccggc gcggctgggc
     1141 gcgcgcgggg ccgaggtgcc accgaccgcg ccgccgctcc tgcttgcacc tctcccggcg
     1201 gcggcccccg ccaagccact ccgaggcccg gcggccggcg gcgcgcacct gtactgcccc
     1261 ctgcggctgc ccgcagccct gcaggcggcc tcagtccgcc gccctggccc gcacctgccg
     1321 tacccggtgg agacgctcct agcctgagta gcgccctcga gcctctagaa ctatagtgag
     1381 tcgtattacg tagatccaga catgataaga tacattgatg agtttggaca aaccacaact
     1441 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     1501 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     1561 gttcaggggg aggtgtggga ggttttttaa ttcgcggccg cggcgccaat gcattgggcc
     1621 cggtacccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     1681 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     1741 ggagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     1801 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     1861 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     1921 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1981 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2041 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2101 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2161 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2221 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2281 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2341 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2401 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2461 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2521 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2581 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2641 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2701 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2761 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2821 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2881 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2941 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3001 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3061 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3121 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3181 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3241 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3301 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3361 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3421 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3481 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3541 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3601 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3661 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3721 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3781 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     3841 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     3901 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     3961 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     4021 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     4081 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     4141 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     4201 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     4261 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
     4321 agggcgatcg gtgcgggcct cttcgctatt acgccagtcg accgccaatt caatatggcg
     4381 tatatggact catgccaatt caatatggtg gatctggacc tgtgccaatt caatatggcg
     4441 tatatggact cgtgccaatt caatatggtg gatctggacc ccagccaatt caatatggcg
     4501 gacttggcac catgccaatt caatatggcg gacctggcac tgtgccaact ggggaggggt
     4561 ctacttggca cggtgccaag tttgaggagg ggtcttggcc ctgtgccaag tccgccatat
     4621 tgaattggca tggtgccaat aatggcggcc atattggcta tatgccagga tcaatatata
     4681 ggcaatatcc aatatggccc tatgccaata tggctattgg ccaggttcaa tactatgtat
     4741 tggccctatg ccatatagta ttccatatat gggttttcct attgacgtag atagcccctc
     4801 ccaatgggcg gtcccatata ccatatatgg ggcttcctaa taccgcccat agccactccc
     4861 ccattgacgt caatggtctc tatatatggt ctttcctatt gacgtcatat gggcggtcct
     4921 attgacgtat atggcgcctc ccccattgac gtcaattacg gtaaatggcc cgcctggctc
     4981 aatgcccatt gacgtcaata ggaccaccca ccattgacgt caatgggatg gctcattgcc
     5041 cattcatatc cgttctcacg ccccctattg acgtcaatga cggtaaatgg cccacttggc
     5101 agtacatcaa tatctattaa tagtaacttg gcaagtacat tactattgga agtacgccag
     5161 ggtacattgg cagtactccc attgacgtca atggcggtaa atggcccgcg atggctgcca
     5221 agtacatccc cattgacgtc aatggggagg ggcaatgacg caaatgggcg ttccattgac
     5281 gtaaatgggc ggtaggcgtg cctaatggga ggtctatata agcaatgctc gtttagggaa
     5341 c
//
