LOCUS       sequence-299563-        9437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDECKO_GFP_AAVS1_1801. Targeting, non-essential locus control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Esposito R, Polidori T, Meise DF, Pulido-Quetglas C, Chouvardas P, 
            Forster S, Schaerer P, Kobel A, Schlatter J, Kerkhof E, Roemmele M, 
            Rice ES, Zhu L, Lanzos A, Guillen-Ramirez HA, Basile G, Carrozzo I, 
            Vancura A, Ullrich S, Andrades A, Harvey D, Medina PP, Ma PC, 
            Haefliger S, Wang X, Martinez I, Ochsenbein AF, Riether C, Johnson R
  TITLE     Multi-hallmark long noncoding RNA maps reveal non-small cell lung 
            cancer vulnerabilities.
  JOURNAL   Cell Genom. 2022 Aug 22;2(9):100171. doi: 
            10.1016/j.xgen.2022.100171. eCollection 2022 Sep 14.
  PUBMED    36778670
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2022-08-22"; volume: "2(9):100171. doi"; pages: " 
            10.1016/j.xgen.2022.100171. eCollection 2022 Sep 14"
COMMENT     Sequence Label: pDECKO_GFP_AAVS1
FEATURES             Location/Qualifiers
     source          1..9437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        271..346
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        353..567
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     488..507
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        596..671
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    727..844
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        983..2161
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1214..2152
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2109..2129
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2174..2770
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2174..2193)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2670..2690
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2780..2833
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2840..2849
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2846..2848,2849..2851,2852..3562)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKLSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2891..2912)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3152..3171)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3499..3520
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3578..4166
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3631..3651)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4049..4060)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4238..4471
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4549..4670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4586..4605)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4640..4659
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4710..4845
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4865..4884)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4866..4884)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4894..4911)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4894..4910)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4903..4925)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5052..5507
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5139..5158)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5349..5370
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5533..5637
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5638..5706,5707..6498)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5856..5875)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6669..7257
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7158..7177
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7411..7428
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7545..7566
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7581..7586,7587..7604,7605..7611)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7619..7635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7624..7646
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7643..7659
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7643..7659
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7678..7698
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7680..7698
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7726..7952
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7953..8133
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8180..8305
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8798..9031
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9216..9260
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg gagtgccctt gctgtgccgc gttttagagc tagaaatagc aagttaaaat
      301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaacgctg
      361 acgtcatcaa cccgctccaa ggaatcgcgg gcccagtgtc actaggcggg aacacccagc
      421 gcgcgtgcgc cctggcagga agatggctgt gagggacagg ggagtggcgc cctgcaatat
      481 ttgcatgtcg ctatgtgttc tgggaaatca ccataaacgt gaaatgtctt tggatttggg
      541 agtcttataa gttctgtatg agaccactct ttccccctct gggggatgca ggggagtttt
      601 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
      661 cgagtcggtg cttttttaag cttggcgtaa ctagatcttg agacaaatgg cagtattcat
      721 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
      781 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
      841 ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg agggggcccg
      901 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
      961 ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc
     1021 cacagtcccc gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg
     1081 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     1141 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     1201 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
     1261 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga
     1321 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
     1381 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
     1441 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     1501 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     1561 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     1621 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     1681 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     1741 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     1801 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     1861 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     1921 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     1981 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     2041 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     2101 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     2161 acgtacggcc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     2221 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     2281 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     2341 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
     2401 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     2461 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     2521 ccggcccaag gagcccgcgt ggttcctggc caccgtcgga gtctcgcccg accaccaggg
     2581 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     2641 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     2701 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     2761 gcccggtgcc ggatcgggag agggcagagg aagtctgcta acatgcggtg acgtcgagga
     2821 gaatcctggc ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     2881 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagctca gcgtgtccgg
     2941 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     3001 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     3061 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3121 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3181 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3241 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3301 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     3361 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3421 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     3481 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     3541 cggcatggac gagctgtaca agacgcgtta agtcgacaat caacctctgg attacaaaat
     3601 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     3661 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3721 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3781 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3841 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3901 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3961 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     4021 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4081 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4141 gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct
     4201 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     4261 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     4321 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4381 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4441 cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt
     4501 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     4561 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     4621 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     4681 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4741 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4801 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     4861 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     4921 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     4981 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     5041 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5101 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5161 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5221 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5281 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5341 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5401 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5461 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     5521 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5581 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5641 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5701 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5761 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5821 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5881 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5941 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     6001 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6061 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6121 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6181 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6241 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6301 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6361 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6421 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6481 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6541 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6601 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6661 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6721 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6781 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6841 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6901 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6961 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7021 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7081 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7141 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7201 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7261 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7321 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7381 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7441 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     7501 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     7561 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     7621 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     7681 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     7741 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     7801 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     7861 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     7921 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
     7981 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8041 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8101 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     8161 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     8221 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     8281 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     8341 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     8401 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     8461 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     8521 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     8581 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     8641 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     8701 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     8761 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     8821 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     8881 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     8941 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     9001 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     9061 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     9121 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     9181 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     9241 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     9301 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     9361 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     9421 ccccgagggg acccaga
//
