LOCUS       sequence-299605-        9432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDECKO_mCherry_AAVS1_186. Targeting, non-essential locus control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9432)
  TITLE     Johnson lab sgRNA plasmids
REFERENCE   2  (bases 1 to 9432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDECKO_mCherry_AAVS1
FEATURES             Location/Qualifiers
     source          1..9432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        272..347
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        354..568
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     489..508
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        597..672
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    728..845
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        984..2162
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1215..2153
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2110..2130
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2175..2771
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2175..2194)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2671..2691
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2781..2834
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2841..2850
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2847..3557
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2992..3010)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3260..3279
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3573..4161
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3626..3646)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4044..4055)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4233..4466
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4544..4665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4581..4600)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4635..4654
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4705..4840
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4860..4879)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4861..4879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4889..4906)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4889..4905)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4898..4920)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5047..5502
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5134..5153)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5344..5365
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5528..5632
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5633..5701,5702..6493)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5851..5870)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6664..7252
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7153..7172
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7406..7423
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7540..7561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7576..7581,7582..7599,7600..7606)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7614..7630
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7619..7641
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7638..7654
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7638..7654
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7673..7693
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7675..7693
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7721..7947
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7948..8128
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8175..8300
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8793..9026
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9211..9255
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg gactctttaa ggaaagaagg agttttagag ctagaaatag caagttaaaa
      301 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttgaacgct
      361 gacgtcatca acccgctcca aggaatcgcg ggcccagtgt cactaggcgg gaacacccag
      421 cgcgcgtgcg ccctggcagg aagatggctg tgagggacag gggagtggcg ccctgcaata
      481 tttgcatgtc gctatgtgtt ctgggaaatc accataaacg tgaaatgtct ttggatttgg
      541 gagtcttata agttctgtat gagaccactc tttccctcag tctgaagagc agagccgttt
      601 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
      661 ccgagtcggt gcttttttaa gcttggcgta actagatctt gagacaaatg gcagtattca
      721 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
      781 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
      841 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gagggggccc
      901 gggtgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct
      961 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
     1021 ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc tagagaaggt
     1081 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     1141 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     1201 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     1261 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg
     1321 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc
     1381 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
     1441 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
     1501 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca
     1561 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
     1621 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
     1681 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
     1741 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
     1801 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
     1861 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
     1921 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt
     1981 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     2041 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     2101 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     2161 gacgtacggc caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     2221 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     2281 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     2341 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct
     2401 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     2461 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     2521 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg agtctcgccc gaccaccagg
     2581 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     2641 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     2701 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     2761 agcccggtgc cggatcggga gagggcagag gaagtctgct aacatgcggt gacgtcgagg
     2821 agaatcctgg cccaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca
     2881 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     2941 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     3001 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     3061 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     3121 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     3181 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     3241 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     3301 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     3361 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     3421 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     3481 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     3541 acgagctgta caagtaaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     3601 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     3661 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     3721 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     3781 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     3841 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     3901 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     3961 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4021 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4081 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4141 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     4201 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     4261 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     4321 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4381 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     4441 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
     4501 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     4561 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4621 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     4681 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4741 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     4801 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     4861 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     4921 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     4981 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     5041 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5101 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5161 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5221 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5281 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5341 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5401 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5461 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     5521 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5581 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5641 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5701 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5761 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5821 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     5881 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5941 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6001 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6061 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6121 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6181 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6241 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6301 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6361 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6421 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6481 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6541 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6601 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6661 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6721 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6781 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6841 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6901 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6961 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7021 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7081 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7141 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7201 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7261 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7321 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7381 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     7441 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     7501 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     7561 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     7621 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     7681 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     7741 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     7801 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     7861 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     7921 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     7981 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8041 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8101 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     8161 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     8221 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     8281 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     8341 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     8401 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     8461 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     8521 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     8581 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     8641 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     8701 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     8761 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     8821 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     8881 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     8941 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     9001 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     9061 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     9121 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     9181 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     9241 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     9301 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     9361 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     9421 aggggaccca ga
//
