LOCUS       sequence-299631-       10159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AscI endonuclease in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10159)
  AUTHORS   Gnugge R, Symington LS
  TITLE     Efficient DNA double-strand break formation at single or multiple 
            defined sites in the Saccharomyces cerevisiae genome.
  JOURNAL   Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 
            10.1093/nar/gkaa833.
  PUBMED    33053188
REFERENCE   2  (bases 1 to 10159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833."
COMMENT     Sequence Label: pRG645_lexO-AscI_LexA-TF_LEU2MX
FEATURES             Location/Qualifiers
     source          1..10159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2635..2654
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2635..2653
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4436..4456
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4463..4486
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      4536..4785
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             join(5455..5706,5707..6060)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     5998..6019
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             6079..7014
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             6127..7017
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      7481..7730
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(7893..7911)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7932..7948)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7932..7948)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7945..7967)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7956..7972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(8231..8248)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8402..8990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8482..8501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9169..9960,9961..10029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9792..9811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10030..10134)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgaaa gaacgcggca ttagcaacat ttgcgaagcg aacgattttg cgaactgcta
       61 ttaattaact ctggcgcgcc taactttttg tgtggtgccc tcctccttgt caatattaat
      121 gttaaagtgc aattcttttt ccttatcacg ttgagccatt agtatcaatt tgcttacctg
      181 tattccttta catcctcctt tttctccttc ttgataaatg tatgtagatt gcgtatatag
      241 tttcgtctac cctatgaaca tattccattt tgtaatttcg tgtcgcatat gatccgcagg
      301 ctaaccggaa cctgtattat ttagtttatg ctacgttaaa taaagacctt tcgttcacat
      361 aactgaatgt gtaatggcct tgagatttca agcataccaa gttggtggag acggggtcgt
      421 tacaaaagac tctttaagcc aagatttcct tgacagcctt ggcgatagca tcgccaacct
      481 cagtggtaga gttagaacca ccaaggtcac cggttctgac acctgcatcc aagacatttc
      541 taacagcttc ttcaagagcc ctaccttctt caaccaaatc caaggataac ttcaacatca
      601 tagctgcaga taagatggta gcaattgggt taaccttgtt tgctggtaaa tctggggcag
      661 aaccatgaca tggttcgtac aaaccgaatg ccttgttagt gtcaggtagg gaagctagag
      721 atgcagaagg taataaaccc aaagaacctg gaataacaga ggcttcatcg gagataatat
      781 caccaaacat gttgttggta ataacaacac cgtttagctt agttggtgat ttaaccaaaa
      841 tcatagcagc agagtcgatc aattggtgct gaacagttaa ttgtgggaac tcagtcttga
      901 tggtttcttc aacagtcttt ctccacaatc tggaagaggc aagcacgtta gccttgtcaa
      961 gagaccagat tggtaatggt gggttttgtt gcaatgccaa gaaagcagcc attcttgtaa
     1021 ttctttgaac ttcaggaaca ctgtatttct cagagtccca agcaactccg tcaccttcat
     1081 cttcttttct ttcaccaaag tagataccac caaccaattc tctaacgacg acgaaatcag
     1141 taccctttgc atattcaggc ttcaaaggag aaagatctag taaagaatca gaagcaaagt
     1201 tacatggtct caagttggcg tatagaccca attcctttct gatcttcaat agaccttgtt
     1261 ctggtctaac tgcgcccgta ccccattttg gaccaccaac agcacctagt aagacagcat
     1321 cggctttctt agaggcttca agagcttcat ctggtaaagg agtgccagtg gcatcgatgg
     1381 cagcaccccc gatcaagtga tgttggaaat tgaacttaat ttctggacgg acttcagcaa
     1441 tggcattcaa gaccttaata gcttcgtcag taacttcttt accgacgtga tcacccggta
     1501 ggacaacgat attcttagac atggtataat ctgtgtagtg tgggatactt tttacttctt
     1561 caaataggta tcaacaataa aaataaatcg aagcaaatgt aggaatgcgt taaagcagat
     1621 gtacttactt agagtacata gatatattta tataattcaa tatataaaag tatatgaagc
     1681 atctgatgtt gaacctgtca tgactctaaa ctggaatggg tagttatgct tctgaaggtt
     1741 ttccgttaga aaatgagtgc agggatcagg cccggaaccg gttttagcct gaaaggaaaa
     1801 gaaaaaaatg cggtgattac tagcacgtga ctgcgctgaa ttggaatcta ccacaagtac
     1861 agcgaatggc aagagggaag gggaatatat tgtgcctggc ggtagcgtct gctatatcaa
     1921 atgttaaaca taactaaatg cagtagtgaa tagagtaatg tatttctaca tctgtgggta
     1981 gggccaatgg gagcccgatg tctattgtac agatattttc cttttatagt taatcaattc
     2041 aacttggaaa tcattgagtc gatgtctact tggataactg gagccgtggc agcgtttgaa
     2101 atttgacgag tcttcttgtt ttgttcgcgc attttcagtt tattcaaacg ggagatggca
     2161 ccgaatccac tgacttctct gttcaatttg aatggtaata ggaagaaagt acatttcgag
     2221 aaattttcgc ccaacgtcga aatctcatgg atgatttcct cgatacaaat gacaccttca
     2281 tcacctaatt tctcttcaac gatattgttg tcattcaaga ttatctcttt tggttcttta
     2341 tcttctggcc tttgccacat tattctagtt ctcttttgga ttaaagaacg gatagatgca
     2401 agtgaaggtt gtccgataac aatatatggt gatacaagtt tcaacaatgg gtacacgtcc
     2461 ttggtaagtt tgatgaaaac accagtgttc aactcaacaa gtctcaaaag agataacact
     2521 ttgtaagcct ttgctgggat cttcacagcg atcggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
     2641 gactcactat agggcgaatt ggagaaacag aataatatat aaacctgtat aatataacct
     2701 tgaagactat atttcttttc gcggccgcct atagggcgaa ttggagctcc ctaggtgctg
     2761 tatatactca cagcataact gtatatacac ccagggtcta ggtgctgtat atactcacag
     2821 cataactgta tatacaccca gggtctaggt gctgtatata ctcacagcat aactgtatat
     2881 acacccaggg tctaggtgct gtatatactc acagcataac tgtatataca cccagggtct
     2941 agagcatgtg ctctgtatgt atataaaact cttgttttct tcttttctct tctttcctta
     3001 tacattagga cctttgcagc ataaattact atacttctat actagtggat cccccgggct
     3061 gcaggaattc atgattgaat ttccagagta cagagattct tctgctgctc caaaaatctc
     3121 cgatttggaa agattgatgg acaggtcttt gaccaactct caatatgttg atggtgctaa
     3181 cgctgctaga ttattgggta ctttcatcag atccttcaga tccgttattg gttctgctga
     3241 agaatctgct actagagcta atttggttga agctcatgat gaagctaagt tgttcggttt
     3301 gatgttgtct gctggtttcg atttgatctg taacgctgaa tacgttcacg gtagattggt
     3361 taacaacaag tggatctatt gtcacagagg tggtgaacca gcagttgctt attactcttt
     3421 cttgaaacaa tgtccaagat gctgcttgga tagaggtttg gaaggtagat tgtctggtgc
     3481 tcaacataag ccaacttctc atcatattgg tgaaattacc accgttgcta ttgccttgtt
     3541 gttgcaatta gttgctgctg ctaacgaaaa cccattcgaa attgctacta tcaccaagca
     3601 atcccatgat gttgatgcta ttggtttcag agatgacttg ttggtcttgt tcgaaatcaa
     3661 ggcttctcca atggtttctt tcccattggt tactgaattg gaagaaccta tgttgcaaga
     3721 aggtccagac ggtccagttg agtatagaca acattctttg gttgacttga cgttgcaggg
     3781 tagagaattt gctgttgcta ttccacatgc tgaaacagct attccattgg gtgaaagaga
     3841 aggcgaatct tggccatacg aaccattgat tgattacttt tctgttccag ctaatgccgc
     3901 ttcttacttg caagcctgga ttgaattata tgctgcttac agaactccca agactcaaag
     3961 agctggtaga actgctgctt tggcttattt ggttaatggt tggggtgacg aaatcgattc
     4021 taacaaaaca aaaccaggtt tgggtagaac cgatgatgtt aagaaaggta cttaccagtt
     4081 gttgaagttc ggttcctatt acagagatga tgctgcatca gttccagtta gaggtgcttt
     4141 ggttgctaac ttagatcctt tgtttttgag gccaggttac atcgatggtt tgtctgatgt
     4201 tagatggggt catggtagag atttcacttt ggaagaaggt gagtacagaa ttgctgaagg
     4261 ttctttgaga cacttgtacg atgctatttt ggctttcaat gaccctttgt tgaatgaccc
     4321 attgctgcaa gaaatctttg atttgggtgc tgtcgaaaga aagttggcta atggtgattt
     4381 ggaagccttg ttggataagt ggattgctag accagaaata gttttggacc catctccaaa
     4441 gaagaagaga aaagttgata tcgactacaa ggatgacgac gacaagaagc tttaactaac
     4501 taacctcgag acaggcccct tttcctttgt ctatatcatg taattagtta tgtcacgctt
     4561 acattcacgc cctcctccca catccgctct aaccgaaaag gaaggagtta gacaacctga
     4621 agtctaggtc cctatttatt ttttttaata gttatgttag tattaagaac gttatttata
     4681 tttcaaattt ttcttttttt tctgtacaaa cgcgtgtacg catgtaacat tatactgaaa
     4741 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcaaggt tcgcagttta
     4801 cactctcatc gtcgctctca tcatcgcttc cgttgttgtt ttccttagta gcgtctgctt
     4861 ggtacccagc ttcggccgct ctagagaagc gggtaagctg ccacagcaat taatgcacaa
     4921 catttaacct acattcttcc ttatcggatc ctcaaaaccc ttaaaaacat atgcctcacc
     4981 ctaacatatt ttccaattaa ccctcaatat ttctctgtca cccggcctct attttccatt
     5041 ttcttcttta cccgccacgc gtttttttct ttcaaatttt tttcttcctt cttctttttc
     5101 ttccacgtcc tcttgcataa ataaataaac cgttttgaaa ccaaactcgc ctctctctct
     5161 cctttttgaa atatttttgg gtttgtttga tcctttcctt cccaatctct cttgtttaat
     5221 atatattcat ttatatcacg ctctcttttt atcttccttt ttttcctctc tcttgtattc
     5281 ttccttcccc tttctactca aaccaagaag aaaaagaaaa ggtcaatctt tgttaaagaa
     5341 taggatcttc tactacatca gcttttagat ttttcacgct tactgctttt ttcttcccaa
     5401 gatcgaaaat ttactgaatt aacaactagt ggatcccccg ggctgcagga attcatgaaa
     5461 gcgttaacgg ccaggcaaca agaggtgttt gatctcatcc gtgatcacat cagccagaca
     5521 ggtatgccgc cgacgcgtgc ggaaatcgcg cagcgtttgg ggttccgttc cccaaacgcg
     5581 gctgaagaac atctgaaggc gctggcacgc aaaggcgtta ttgaaattgt ttccggcgca
     5641 tcacgcggga ttcgtctgtt gcaggaagag gaagaagggt tgccgctggt aggtcgtgtg
     5701 gctgccggtg aaccacttct ggcgcaacag catattgaag gtcattatca ggtcgatcct
     5761 tccttattca agccgaatgc tgatttcctg ctgcgcgtca gcgggatgtc gatgaaagac
     5821 atcggcatta tggatggtga cttgctggca gtgcataaaa ctcaggatgt acgtaacggt
     5881 caggtcgttg tcgcacgtat tgatgacgag gttaccgtta agcgcctgaa aaaacagggc
     5941 aataaagtcg aactgttgcc agaaaatagc gagtttaaac caattgtcgt agatcttcgt
     6001 cagcagagct tcaccattga agggctggcg gttggggtta ttcgcaacgg cgactggctg
     6061 ggttctaagg atatctctgc tggagacatg agagctgcca acctttggcc aagcccgctc
     6121 atgatcaaac gctctaagaa gaacagcctg gccttgtccc tgacggccga ccagatggtc
     6181 agtgccttgt tggatgctga gccccccata ctctattccg agtatgatcc taccagaccc
     6241 ttcagtgaag cttcgatgat gggcttactg accaacctgg cagacaggga gctggttcac
     6301 atgatcaact gggcgaagag ggtgccaggc tttgtggatt tgaccctcca tgatcaggtc
     6361 caccttctag aatgtgcctg gttagagatc ctgatgattg gtctcgtctg gcgctccatg
     6421 gagcacccag tgaagctact gtttgctcct aacttgctct tggacaggaa ccagggaaaa
     6481 tgtgtagagg gcatggtgga gatcttcgac atgctgctgg ctacatcatc tcggttccgc
     6541 atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat ctattatttt gcttaattct
     6601 ggagtgtaca catttctgtc cagcaccctg aagtctctgg aagagaagga ccatatccac
     6661 cgagtcctgg acaagatcac agacactttg atccacctga tggccaaggc aggcctgacc
     6721 ctgcagcagc agcaccagcg gctggcccag ctcctcctca tcctctccca catcaggcac
     6781 atgagtaaca aaggcatgga gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc
     6841 tatgacctgc tgctggagat gctggacgcc caccgcctac atgcgcccac tagccgtgga
     6901 ggggcatccg tggaggagac ggaccaaagc cacttggcca ctgcgggctc tacttcatcg
     6961 cattccttgc aaaagtatta catcacgggg gaggcagagg gtttccctgc cacagtcgat
     7021 atcatgcagg atctgccggg caacgataac agcaccgcgg gcgaatttcc aggtattact
     7081 ttgagaatcc aagaaactga tatgttgtat aaaggtgata ctttgtattt ggattggttg
     7141 gaagatggta ttgctgaatt ggttttcgat gctccaggtt ctgttaataa attggatact
     7201 gctgttgctt ctttgggtga agctattggt gttttggaac aacaatctga tttgatttgg
     7261 gaaactttga ctgttaaaga tgctaaagtt aatttcgatt ctggtttgga aaaattcgaa
     7321 gaagctattc catctgctga tgatttcgat ccagttgctg aaagaagatc ctctggtgaa
     7381 tttagagctg aaagacattc tggtggtact gatttgtgtt tcaagcttta aacgcgttta
     7441 aagcttatcc tcgagacagg ccccttttcc tttgtctata tcatgtaatt agttatgtca
     7501 cgcttacatt cacgccctcc tcccacatcc gctctaaccg aaaaggaagg agttagacaa
     7561 cctgaagtct aggtccctat ttattttttt taatagttat gttagtatta agaacgttat
     7621 ttatatttca aatttttctt ttttttctgt acaaacgcgt gtacgcatgt aacattatac
     7681 tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc aaggttcgca
     7741 gtttacactc tcatcgtcgc tctcatcatc gcttccgttg ttgttttcct tagtagcgtc
     7801 tgcttggtac ccagcttcgg ccgtatttac agataacgat gtcgttatta tatatatata
     7861 tatatattgt cacttgtacc cagcttttgt tccctttagt gagggttaat ttcgagcttg
     7921 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aacagctgta
     7981 atatatagta gtaacctgaa aaatagtcaa tgcccgtggt tcatttgcca atagaacagt
     8041 cgatgtgatc aatgtacacg aaagtcaata taaaatatcc aaatagccaa tggtgtcagg
     8101 acggctaagg caaatgcaac tttgaaatac ttattcggtt tgcatcttgg cgcgcctctt
     8161 cctgcaggca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8221 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8281 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8341 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8401 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8461 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8521 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8581 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8641 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8701 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8761 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8821 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8881 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8941 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9001 tgatcttttg tttaaaccta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9061 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9181 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9241 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9301 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9361 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9421 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9481 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9541 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9601 cggtcctccg atggttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9661 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9721 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9781 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9841 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9901 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9961 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10021 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10081 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10141 tccccgaaaa gtgccacct
//
