LOCUS       sequence-299703-        6283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid has been discontinued and a new version is now 
            available. Please see: pGFAP::MyrGFP_v2 (Plasmid #160277) 
            https://www.addgene.org/160277/.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Nam HS, Benezra R
  TITLE     High levels of Id1 expression define B1 type adult neural stem 
            cells.
  JOURNAL   Cell Stem Cell. 2009 Nov 6. 5(5):515-26.
  PUBMED    19896442
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Nov 6. 5(5):515-26."
COMMENT     Sequence Label: pGFAP::MyrGFP
FEATURES             Location/Qualifiers
     source          1..6283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(2603..2605,2606..3313)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     CDS             join(2603..2605,2606..3313)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(2645..2666)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2906..2925)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3253..3274
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3887..3904)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3887..3903)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3896..3918)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4112..4131)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4231..4253
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4291..4309)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4377..4481
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4482..4550,4551..5342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5513..6101
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6002..6021
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6255..6272
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgagctc
      241 ggtaccagat ctgagctccc acctccctct ctgtgctggg actcacagag ggagacctca
      301 ggaggcagtc tgtccatcac atgtccaaat gcagagcata ccctgggctg ggcgcagtgg
      361 cgcacaactg taattccagc actttgggag gctgatgtgg aaggatcact tgagcccaga
      421 agttctagac cagcctgggc aacatggcaa gaccctatct ctacaaaaaa agttaaaaaa
      481 tcagccacgt gtggtgacac acacctgtag tcccagctat tcaggaggct gaggtgaggg
      541 gatcacttaa ggctgggagg ttgaggctgc agtgagtcgt ggttgcgcca ctgcactcca
      601 gcctgggcaa cagtgagacc ctgtctcaaa agacaaaaaa aaaaaaaaaa aaaaaaagaa
      661 catatcctgg tgtggagtag gggacgctgc tctgacagag gctcgggggc ctgagctggc
      721 tctgtgagct ggggaggagg cagacagcca ggccttgtct gcaagcagac ctggcagcat
      781 tgggctggcc gccccccagg gcctcctctt catgcccagt gaatgactca ccttggcaca
      841 gacacaatgt tcggggtggg cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa
      901 tgccttccga gaagcccatt gagcaggggg cttgcattgc accccagcct gacagcctgg
      961 catcttggga taaaagcagc acagccccct aggggctgcc cttgctgtgt ggcgccaccg
     1021 gcggtggaga acaaggctct attcagcctg tgcccaggaa aggggatcag gggatgccca
     1081 ggcatggaca gtgggtggca gggggggaga ggagggctgt ctgcttccca gaagtccaag
     1141 gacacaaatg ggtgagggga ctgggcaggg ttctgaccct gtgggaccag agtggagggc
     1201 gtagatggac ctgaagtctc cagggacaac agggcccagg tctcaggctc ctagttgggc
     1261 ccagtggctc cagcgtttcc aaacccatcc atccccagag gttcttccca tctctccagg
     1321 ctgatgtgtg ggaactcgag gaaataaatc tccagtggga gacggagggg tggccaggga
     1381 aacggggcgc tgcaggaata aagacgagcc agcacagcca gctcatgtgt aacggctttg
     1441 tggagctgtc aaggcctggt ctctgggaga gaggcacagg gaggccagac aaggaagggg
     1501 tgacctggag ggacagatcc aggggctaaa gtcctgataa ggcaagagag tgccggcccc
     1561 ctcttgccct atcaggacct ccactgccac atagaggcca tgattgaccc ttagacaaag
     1621 ggctggtgtc caatcccagc ccccagcccc agaactccag ggaatgaatg ggcagagagc
     1681 aggaatgtgg gacatctgtg ttcaagggaa ggactccagg agtctgctgg gaatgaggcc
     1741 tagtaggaaa tgaggtggcc cttgagggta cagaacaggt tcattcttcg ccaaattccc
     1801 agcaccttgc aggcacttac agctgagtga gataatgcct gggttatgaa atcaaaaagt
     1861 tggaaagcag gtcagaggtc atctggtaca gcccttcctt cccttttttt tttttttttt
     1921 ttgtgagaca aggtctctct ctgttgccca ggctggagtg gcgcaaacac agctcactgc
     1981 agcctcaacc tactgggctc aagcaatcct ccagcctcag cctcccaaag tgctgggatt
     2041 acaagcatga gccaccccac tcagcccttt ccttcctttt taattgatgc ataataattg
     2101 taagtattca tcatggtcca accaaccctt tcttgaccca ccttcctaga gagagggtcc
     2161 tcttgcttca gcggtcaggg ccccagaccc atggtctggc tccaggtacc acctgcctca
     2221 tgcaggagtt ggcgtgccca ggaagctctg cctctgggca cagtgacctc agtggggtga
     2281 ggggagctct ccccatagct gggctgcggc ccaaccccac cccctcaggc tatgccaggg
     2341 ggtgttgcca ggggcacccg ggcatcgcca gtctagccca ctccttcata aagccctcgc
     2401 atcccaggag cgagcagagc cagagcaggt tggagaggag acgcatcacc tccgctgctc
     2461 gcggggatcc accatgggtt gctgtttctc caagaccgca gcgaagggag aagccgccgc
     2521 ggagaggcct ggggaggcgg ctgtggcctc gtcgccttcc aaagcgaacg gacaggagaa
     2581 tggccacgtg aaggtaggat ccgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     2641 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     2701 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     2761 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     2821 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     2881 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     2941 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     3001 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     3061 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     3121 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     3181 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     3241 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     3301 ggacgagctg tacaataagg atccgcagat cccggccaga taccgatgct gccgcagcaa
     3361 aagcaggagc agatgccgcc gtcgcaggcg aagatgtcgc agacggagga ggcgatgctg
     3421 ccggcggagg aggcgaagta agtagagggc tgggctgggc tgtggggggt gtggggtgcg
     3481 ggactgggca gtctgggagt ccctctcacc acttttctta cctttctagg atgctgccgt
     3541 cgccgccgct catacaccat aaggtgtaaa aaatactaga tgcacagaat agcaagtcca
     3601 tcaaaactcc tgcgtgagaa ttttaccaga cttcaagagc atctcgccac atcttgaaaa
     3661 atgccaccgt ccgatgaaaa acaggagcct gctaaggaac aatgccacct gtcaataaat
     3721 gttgaaaact catcccattc ctgcctcttg gtccttgggc ttggggaggg gtgcgcggat
     3781 gtggttaggg aacatgactg gtcaaatggg aagggcttca aaagaattcc caatattgac
     3841 taccaagcca cctgtacaga tctgcctgca ggcatgcaag cttggcactg gccgtcgttt
     3901 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     3961 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     4021 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     4081 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     4141 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     4201 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     4261 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     4321 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     4381 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4441 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4501 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     4561 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     4621 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     4681 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     4741 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4801 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4861 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4921 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4981 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5041 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5101 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5161 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5221 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     5281 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     5341 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     5401 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5461 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5521 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5581 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5641 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5701 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5761 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5821 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5881 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5941 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6001 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6061 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6121 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6181 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6241 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aga
//
