LOCUS       sequence-299705-        6893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Nam HS, Benezra R
  TITLE     High levels of Id1 expression define B1 type adult neural stem 
            cells.
  JOURNAL   Cell Stem Cell. 2009 Nov 6. 5(5):515-26.
  PUBMED    19896442
REFERENCE   2  (bases 1 to 6893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Nov 6. 5(5):515-26."
COMMENT     Sequence Label: pGFAP::MyrTomato
FEATURES             Location/Qualifiers
     source          1..6893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2498..3928
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(4497..4514)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4497..4513)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4506..4528)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4722..4741)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4841..4863
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4901..4919)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4987..5091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5092..5160,5161..5952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6123..6711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6612..6631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6865..6882
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgagctc
      241 ggtaccagat ctgagctccc acctccctct ctgtgctggg actcacagag ggagacctca
      301 ggaggcagtc tgtccatcac atgtccaaat gcagagcata ccctgggctg ggcgcagtgg
      361 cgcacaactg taattccagc actttgggag gctgatgtgg aaggatcact tgagcccaga
      421 agttctagac cagcctgggc aacatggcaa gaccctatct ctacaaaaaa agttaaaaaa
      481 tcagccacgt gtggtgacac acacctgtag tcccagctat tcaggaggct gaggtgaggg
      541 gatcacttaa ggctgggagg ttgaggctgc agtgagtcgt ggttgcgcca ctgcactcca
      601 gcctgggcaa cagtgagacc ctgtctcaaa agacaaaaaa aaaaaaaaaa aaaaaaagaa
      661 catatcctgg tgtggagtag gggacgctgc tctgacagag gctcgggggc ctgagctggc
      721 tctgtgagct ggggaggagg cagacagcca ggccttgtct gcaagcagac ctggcagcat
      781 tgggctggcc gccccccagg gcctcctctt catgcccagt gaatgactca ccttggcaca
      841 gacacaatgt tcggggtggg cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa
      901 tgccttccga gaagcccatt gagcaggggg cttgcattgc accccagcct gacagcctgg
      961 catcttggga taaaagcagc acagccccct aggggctgcc cttgctgtgt ggcgccaccg
     1021 gcggtggaga acaaggctct attcagcctg tgcccaggaa aggggatcag gggatgccca
     1081 ggcatggaca gtgggtggca gggggggaga ggagggctgt ctgcttccca gaagtccaag
     1141 gacacaaatg ggtgagggga ctgggcaggg ttctgaccct gtgggaccag agtggagggc
     1201 gtagatggac ctgaagtctc cagggacaac agggcccagg tctcaggctc ctagttgggc
     1261 ccagtggctc cagcgtttcc aaacccatcc atccccagag gttcttccca tctctccagg
     1321 ctgatgtgtg ggaactcgag gaaataaatc tccagtggga gacggagggg tggccaggga
     1381 aacggggcgc tgcaggaata aagacgagcc agcacagcca gctcatgtgt aacggctttg
     1441 tggagctgtc aaggcctggt ctctgggaga gaggcacagg gaggccagac aaggaagggg
     1501 tgacctggag ggacagatcc aggggctaaa gtcctgataa ggcaagagag tgccggcccc
     1561 ctcttgccct atcaggacct ccactgccac atagaggcca tgattgaccc ttagacaaag
     1621 ggctggtgtc caatcccagc ccccagcccc agaactccag ggaatgaatg ggcagagagc
     1681 aggaatgtgg gacatctgtg ttcaagggaa ggactccagg agtctgctgg gaatgaggcc
     1741 tagtaggaaa tgaggtggcc cttgagggta cagaacaggt tcattcttcg ccaaattccc
     1801 agcaccttgc aggcacttac agctgagtga gataatgcct gggttatgaa atcaaaaagt
     1861 tggaaagcag gtcagaggtc atctggtaca gcccttcctt cccttttttt tttttttttt
     1921 ttgtgagaca aggtctctct ctgttgccca ggctggagtg gcgcaaacac agctcactgc
     1981 agcctcaacc tactgggctc aagcaatcct ccagcctcag cctcccaaag tgctgggatt
     2041 acaagcatga gccaccccac tcagcccttt ccttcctttt taattgatgc ataataattg
     2101 taagtattca tcatggtcca accaaccctt tcttgaccca ccttcctaga gagagggtcc
     2161 tcttgcttca gcggtcaggg ccccagaccc atggtctggc tccaggtacc acctgcctca
     2221 tgcaggagtt ggcgtgccca ggaagctctg cctctgggca cagtgacctc agtggggtga
     2281 ggggagctct ccccatagct gggctgcggc ccaaccccac cccctcaggc tatgccaggg
     2341 ggtgttgcca ggggcacccg ggcatcgcca gtctagccca ctccttcata aagccctcgc
     2401 atcccaggag cgagcagagc cagagcaggt tggagaggag acgcatcacc tccgctgctc
     2461 gcggggatcc accatgggtt gctgtttctc caagaccatg gtgagcaagg gagaggaggt
     2521 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
     2581 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     2641 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
     2701 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
     2761 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt
     2821 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac
     2881 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
     2941 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct
     3001 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt
     3061 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga
     3121 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca
     3181 tggcaccggc agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat
     3241 ggccgtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca
     3301 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     3361 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     3421 catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct
     3481 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt
     3541 gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg
     3601 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     3661 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct
     3721 gaagctgaag gacggcggcc gctacctggt ggagttcaag accatctaca tggccaagaa
     3781 gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa
     3841 cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct
     3901 gtacggcatg gacgagctgt acaagtaagg atccgcagat cccggccaga taccgatgct
     3961 gccgcagcaa aagcaggagc agatgccgcc gtcgcaggcg aagatgtcgc agacggagga
     4021 ggcgatgctg ccggcggagg aggcgaagta agtagagggc tgggctgggc tgtggggggt
     4081 gtggggtgcg ggactgggca gtctgggagt ccctctcacc acttttctta cctttctagg
     4141 atgctgccgt cgccgccgct catacaccat aaggtgtaaa aaatactaga tgcacagaat
     4201 agcaagtcca tcaaaactcc tgcgtgagaa ttttaccaga cttcaagagc atctcgccac
     4261 atcttgaaaa atgccaccgt ccgatgaaaa acaggagcct gctaaggaac aatgccacct
     4321 gtcaataaat gttgaaaact catcccattc ctgcctcttg gtccttgggc ttggggaggg
     4381 gtgcgcggat gtggttaggg aacatgactg gtcaaatggg aagggcttca aaagaattcc
     4441 caatattgac taccaagcca cctgtacaga tctgcctgca ggcatgcaag cttggcactg
     4501 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     4561 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     4621 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     4681 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     4741 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     4801 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4861 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     4921 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4981 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5041 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5101 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5161 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5221 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5281 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5341 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5401 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5461 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5521 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5581 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5641 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5701 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5761 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5821 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5881 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5941 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6001 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6061 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6121 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6181 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6241 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     6301 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6361 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6421 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6481 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6541 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6601 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6661 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6721 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6781 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6841 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aga
//
