LOCUS       sequence-299727-       10695 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector that can be modified to add gene(s) of interest to C. elegans
            ChrII:8420157 site via CRISPR with SEC selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10695)
  AUTHORS   Stevenson ZC, Moerdyk-Schauwecker MJ, Jamison B, Phillips PC
  TITLE     Rapid Self-Selecting and Clone-Free Integration of Transgenes into 
            Engineered CRISPR Safe Harbor Locations in Caenorhabditis elegans.
  JOURNAL   G3 (Bethesda). 2020 Aug 19. pii: g3.120.401400. doi: 
            10.1534/g3.120.401400.
  PUBMED    32816924
REFERENCE   2  (bases 1 to 10695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 19. pii: g3.120.401400. doi: 
            10.1534/g3.120.401400."
COMMENT     Sequence Label: pMS4 (Empty insertion vector with SEC)
FEATURES             Location/Qualifiers
     source          1..10695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(146..734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(226..245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(905..1696,1697..1765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1528..1547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1766..1870)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1938..1956
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1994..2016)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2116..2135
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    complement(3731..3764)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3774..3803
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(5620..5943)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    7861..7885
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7888..8913
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7888..8913
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(8914..8938)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9291..9324)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tgtgaaattg ttatccgctg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
       61 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag accaggaacc
      121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
      601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1021 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1861 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     1921 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     1981 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2041 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2101 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2161 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     2221 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2281 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agcacgtgtg tttttgaaca
     2341 cggcgatatg tatctgtaga tcatccccaa ttttcaacaa tgaatctaaa tactgaactt
     2401 tttgtgtaat ctttagtgca gaaaaatgac caaaatcagc gattttccga ttatacccaa
     2461 aatatctcag ctcaatgagc acctataaaa atgtaaaaat aaagaaagta gtcgaaccat
     2521 attttgtaaa ggctaaaaaa ctgaataaat ttttatccat ccagcgcggc gttacaatcc
     2581 gtggcccatg tcacgcacgg ctttcaaaga aaaaaaaaca gtctgtttgc gcgacatcga
     2641 tctggcaaaa atcctaaaaa cgactatgcg tggctcactt tatattaata tgaaaaatac
     2701 aaacttggtg gtgcaggtgc tgcagaggcc atcgatgaag aagtggaggc cttgttgtcg
     2761 atctacaaaa ttgtatggtt gtttgagatt ttccttgaaa cgctctcaat cttggatatg
     2821 atataaattc tggataagat atgatggata tgatctatgt ttgatagagt gcatttgaaa
     2881 ggataacggc cctgcagaca tttttgaaaa aataacaaac atttaatttt ctcactcgtt
     2941 taggctattc ccctattttt gatattcctt cgcacatatg aaaactactt tttttcgaaa
     3001 ctgttaactc cagaatttat aaatctatag cccttacttg attatttatt atcatggtta
     3061 ctcacctcat gtctctttct cttttcaaga agtcttcgtt catatctgtt gtctctttcc
     3121 tccttttgaa tctctgcttt gctcttcgcc attgtttcct gaaaataatg taacttgaat
     3181 tgtgtaatat acttttttaa tttgaatttg gcttgtaacg cggaatcact acgtgcggga
     3241 tcatttctta ctagaaaacc cagaaaatgc catatttcac ttatctcggg gtcatttcta
     3301 attagaaaag ctacaaaaac catttctaat tacggtgact ttagaactgg tcgaaatagc
     3361 taattagaaa tgggtttttg ctaattagag gtgactctat gttatctgag taagcctcgt
     3421 ttgtatgtaa actcacagta ctaataaatg caagacaccc gggtttgtct agatatgaaa
     3481 taattgaaat atcaattctg acagacaata atggtaatct tgataaggag ttccacgccc
     3541 aggagaacac gttagttttc ttgtttttga ttgcgtgcgt tattttggag aaaaactcga
     3601 ttttttacaa aataattttt tgaaaggaac actgttcaat aagttttgtc ttttttctca
     3661 gttgtgatac ggttttttat tcttttttgt agttatacag aagaccgcct gcaggagctg
     3721 taagtttaaa ataacttcgt atagcataca ttatacgaag ttattttcag ggagagcaaa
     3781 agcttatctc cgaggaggac ctctaaggat gatcgacgcc aacgtcgttg aattttcaaa
     3841 ttttaaatac tgaatatttg ttttttttcc tattatttat ttattctctt tgtgtttttt
     3901 ttcttgcttt ctaaaaaatt aattcaatcc aaatctaaac attttttttt ctctttccgt
     3961 ctcccaattc gtattccgct cctctcatct gaacacaatg tgcaagttta tttatcttct
     4021 cgctttcatt tcattaggac gtggggggaa ttggtggaag ggggaaacac acaaaaggat
     4081 gatggaaatg aaataaggac acacaatatg caacaacatt caattcagaa atatggagga
     4141 aggtttaaaa gaaaacataa aaatatatag aggaggaagg aaactagctg ccctcgattg
     4201 tctatcttcc attctcacca agtttcaaca actttattca gtcattcttc tctggttcat
     4261 ttcccaacca atcttcccga ataaagccca tctgtccttt cggccacatt cctcacctac
     4321 atccgtactc cttatctccc gttcttatcc agtcttttat cgtctctgcg tctcgcacac
     4381 tgtctcgtcg gtaatgtaaa aaaaaaacaa aaaaaacttg aaaacttgcg gaaagatttc
     4441 ctaataaaag gagacgaatc cggtaggaaa tcgacaagtc tctgcttctc gaagactagg
     4501 tgagttacat ttctttggcc gaattatttt gaaatcctaa atttgataat ttcagagatg
     4561 tctgtaaaac ttgcgtgtta tgtgacggct tcggtcactg tcgccactct tatggtatgc
     4621 ttcatgacca tgtcaaccat ctactcggag gttgatggat tcagagagaa gcttgatacc
     4681 gagatgaatg tgttcagagt gagttaaatt atattttgaa ttttaaataa tttttaattt
     4741 tctagcaatc taccaatgga ttgtggaagg acatagttgt catcggaaga tctagcaagc
     4801 gtgtccgttg tcaatatgaa gagaccaacg ctaccccaac tccacatgct gatggatccc
     4861 catctgctcc accaggtcaa ccaccagcag ttccaccagt cttcaaccag ccaaagactc
     4921 caaatggagc caatggaaat ggaccaacct gcaactgcaa tgctgataac aagtgcccag
     4981 ctggaccatc cggaccaaag ggagttccag gagttccagg actcgacgga gttccaggac
     5041 ttgacggtgt tccaggagtt ggagctgatg atatcgctcc acaacgcgag tctgtcggat
     5101 gcttcacttg cccacaagga ccagttggac caccaggagc tcttggaaga ccaggaccac
     5161 gtggacttcc aggaccaaga ggacaaaatg gaaacccagg aagagatgga caaccaggac
     5221 atccaggaga gcaaggatca tccggtcaaa tcggaaagat cggagagcca ggaccaccag
     5281 gagagaaggg acgcgacgcc gagcatccaa tcggaagacc aggaccaaag ggaccaagag
     5341 gagatcaagg accaacagga ccagctggac agaacggtct tcacggacca ccaggagagc
     5401 caggaaccgt tggaccagaa ggaccatctg gaaagcaagg acgtcaagga ccagacggaa
     5461 cccagggaga gactggacca gacggaagac caggaaagga tgccgagtac tgccagtgcc
     5521 cagacaagtc tccaccatca gaggctgtca acgccaaccg tggatacaga aatatctaaa
     5581 ttgttggtgt tttctaataa aatatttgag attcagtgag acttttttct tggcggcaca
     5641 ataaagtttc ctctttttgt tgggtcactc aacgaacaca atacaaatat ttgagcgctc
     5701 gcatttttct caacctttcg ggtttaaagc acttcaaaat ggaattgaag agagattgtg
     5761 aaagtacatc agacagaatc acattttgat agcattcact tcactcagat gcaaactttt
     5821 cagtgactat aaaaagtcat aaaataataa cattaaaaaa gcaaataaat tagcgagaga
     5881 attttttgac aaaaagaaag aagagtgata gagaagaagg gaatgcttga aaggattttg
     5941 catttatctt aatcaccgtc ttcgagaaga cgaaccatgg cgccagtttc agaatccaag
     6001 tttctaatat agttcatcac aatgttgacg ttggtccaac ctccggcttg cataatctct
     6061 gggatggaaa ctccagcgcg agccatgtca cgggcagctc cgacacgggc ggaatgtcca
     6121 gaccaagcca agtaacgttg tccggaatca tccttggctc cgtagatgag acggtgggta
     6181 gcttcgaaga ttcctgaaaa tttattttca gcttattctt agctaatatg ttgtttaaac
     6241 ttaccttcaa gagctcgagt cgagagttgt gatgttgcac tcggggcagc aactccgttc
     6301 ttgcgaacac gacagaacag gtagttgttt ggatcatcgg cgactccgga aaccgaaatc
     6361 cagcgctcaa cgagcttggt aactccaagg gagagcgctt tttcgactcc ggcagtcgag
     6421 accagcgttt ttgtacgtcc gatgtggatg agcattcttc ctccgtctgt gcgactaata
     6481 tccttgacgc gaatgcgggc aatttcggcg atacggagaa gtgtgttgta agcaattccg
     6541 aggaaggcga ggtttcggat gtcctggcaa cgatcggaat tttccataag agaacggacc
     6601 tgatcgaagt cggttctgaa aatttcgtca agtcttagga ttgaatacat taatatttaa
     6661 acttaccttt cgaaggcgag ggcttgcttg gctctctctc cagcatcaac attttccttg
     6721 cggatacgtc tcattacgag agatacggca ttagaatctg atgggcgtgg aagtccagat
     6781 cggcgatgga gcatattgag ctgtccgaga tgttgttgga tcgtcttcac cgccaatcca
     6841 cgagcttgga ggtagagcaa gtagtcacga acgtcttctg gctcggctgg gaaccacttg
     6901 cggttgttaa gcttgcacca ggcagcccaa gaacggcaca ctgagagaag catcttccag
     6961 gtgtgctcgg agaaggcttg tctgtcacgg aacatatcca tgaggttctt gcgaacttca
     7021 tcacttgtcg cgtcaactgg aagtgctgga agattttgat gaacagtgag aagatttgac
     7081 atattttcga agttttttag atgcactaga acaaagcgtg ttggcttcct ctgagcccgc
     7141 tttccttata tacccgcatt ctgcagcctt acagaatgtt ctagaaggtc ctagatgcat
     7201 tcgtttgaaa atactcccgg tgggtgcaaa gagacgcaga cggaaaatgt atctgggtct
     7261 ctttattgtg tacactactt ttccatgtac cgaatgtgag tcgccctcct tttgcaacaa
     7321 gcagctcgaa tgttctagaa aaaggtggaa aatagtataa ataccgttga aaataaatac
     7381 cgaacaacat ttgctctaat tgtgaaatta gaaatcttca aactataatc atgtcacttt
     7441 accactattt ccgtccagct caacgttccg tttttggtga tcatttttgc tttcgtcgta
     7501 aatctacaca cgcgtctctt ccgtgcgaga gtccaagcca gcagccaaat tcgttgactg
     7561 agtattcaac gtttatacgt tgtcggcaac gagaaatagg aaaatgcatc gggaaatgtt
     7621 cttttttcga ttttttccaa ggttttgaca aattttacca cgaattttgc tatgttttca
     7681 attaaaaaat atgttattca actgtttcta tgaggaaaat aaggctttgc atgtaatttt
     7741 cttattcagc ataattttta attaatttga attttctgtc ctaacgttta ttttgttttc
     7801 ttggttatga ctgatctgaa attaattttt gaattttaag gtaatatgtc aggcggtgcc
     7861 gcaagtttgt acaaaaaagc aggctccatg aaaaagcctg aactcaccgc gacgtctgtc
     7921 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     7981 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     8041 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     8101 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     8161 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     8221 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     8281 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     8341 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     8401 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     8461 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     8521 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     8581 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     8641 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     8701 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     8761 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     8821 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     8881 cgccccagca ctcgtccgag ggcaaaggaa tagacccagc tttcttgtac aaagtgggtc
     8941 caattactct tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg
     9001 ttattatcaa aaaacttctc ttaatttctt tgttttttag cttcttttaa gtcacctcta
     9061 acaatgaaat tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat
     9121 tgtgctccct ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta
     9181 cacttcttat gttttttact tctgataaat ttttttgaaa catcatagaa aaaaccgcac
     9241 acaaaatacc ttatcatatg ttacgtttca gtttatgacc gcaattttta ataacttcgt
     9301 atagcataca ttatacgaag ttattttcag ggagccggat ctgagctctt aagacgaagt
     9361 gagtttgcta ccatcatgga atcaggagga catttcgaca atgtcatata atgtttgaaa
     9421 aatgatttta acaagggtct cattcgagat aagcaatgct ctgagcaagt ggctcgtcgt
     9481 cttcaagaat ctcttgttga tattcatttg gatcctgttt tgcacgaagc atgtgccatg
     9541 gatattcaga gtttttgcaa aaaagttccg ccaggtcaaa caatcttttt aatttttata
     9601 tgatacttcg ggtgtatttt tcttcttttt cctgttcatt ttcgcttttt tctcactttt
     9661 ttgtaacaaa atctctcact tttctgtaag tcttaacaaa agttcaattg gctcgatcaa
     9721 gtaaaatttc ggccgtatga acgttttcaa aggttttcat ttttttttaa gttcattcac
     9781 aatatcatga atctctcgtc ctttggtttc cggtaagtag cgaaataata taagcatact
     9841 tataaatgaa ggaattgaat atagaataag gaaagcataa ctaccaataa cggaatatag
     9901 aggtaatact gtaaatgtac tgataaccac cattacagta ttcattgaat atgccacaga
     9961 ttgagcaata ctacgatgtt tttgaggtac cagttcagag gatataaacc acgaaattgg
    10021 accaactcca gttctgaaat ttcattattc gattaaatca aaatatattt gaaaaacctc
    10081 acccataagt ataaccgtag atgatcaatg caactaggca tccgtatttc atcgggtcaa
    10141 tcagaatacg tatttttgcg aagaatacga atgcaattag agctaaatta ttgagaaatg
    10201 tgaagagaag gagaagcttg cgtcgaccaa ctctgtaaac ttattcatac aattttgtct
    10261 tcaaattttg aacgtttaaa actatcttat tcaatgagtt agcgggtttt tatagtgagt
    10321 tagttcacga ttatctaata aaactatgtc caaacatcct aactgcgacc gtattctcac
    10381 taactcgtct taaaaacgtt gagattattt taaacccttc ttcagtacaa ctacagtact
    10441 gatgcacaaa tacaataata ataatacatt cagacatact ctgaacaatg taacttgttt
    10501 ttgttatact gtggtaacat ttattgaaat gaaatataac cttacttttc aatcacaatt
    10561 ccaccagtaa ttgttccaga aacataagct aaacccattg cagttgtact ccattcagca
    10621 atttctgact caacatttgc attttctaga aagaaagtag aggataaaag aagagcccat
    10681 aatccgacag ggccc
//
