LOCUS       sequence-29973-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Vassallo MF, Tanese N
  TITLE     Isoform-specific interaction of HP1 with human TAFII130.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Apr 30. 99(9):5919-24.
  PUBMED    11959914
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Apr 30. 99(9):5919-24."
COMMENT     Sequence Label: pcDNA HA HP1 beta
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             106..132
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(725..743)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(726..743)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(763..780)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatcca ccatgtaccc atacgatgtt
      121 ccagattacg ctgaattcgg gaaaaaacaa aacaagaaga aagtggagga ggtgctagaa
      181 gaggaggaag aggaatatgt ggtggaaaaa gttctcgacc gtcgagtggt aaagggcaaa
      241 gtggagtacc tcctaaagtg gaagggattc tcagatgagg acaacacatg ggagccagaa
      301 gagaacctgg attgccccga cctcattgct gagtttctgc agtcacagaa aacagcacat
      361 gagacagata aatcagaggg aggcaagcgc aaagctgatt ctgattctga agataaggga
      421 gaggagagca aaccaaagaa gaagaaagaa gagtcagaaa agccacgagg ctttgctcga
      481 ggtttggagc cggagcggat tattggagct acagactcca gtggagagct catgttcctg
      541 atgaaatgga aaaactctga tgaggctgac ctggtccctg ccaaggaagc caatgtcaag
      601 tgcccacagg ttgtcatatc cttctatgag gaaaggctga cgtggcattc ctacccctcg
      661 gaggatgatg acaaaaaaga tgacaagaac taacggcgtc gagcatgcat ctagagggcc
      721 ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta
      781 gttgccagcc atctgttgtt tgcccctc
//
