LOCUS       sequence-299731-        8841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expression of human NHEJ1-S262E/S263E in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Jessulat M, Amin S, Hooshyar M, Malty R, Moutaoufik MT, Zilocchi M, 
            Istace Z, Phanse S, Aoki H, Omidi K, Burnside D, Samanfar B, Aly KA,
            Golshani A, Babu M
  TITLE     The conserved Tpk1 regulates non-homologous end joining 
            double-strand break repair by phosphorylation of Nej1, a homolog of 
            the human XLF.
  JOURNAL   Nucleic Acids Res. 2021 Jul 9. pii: 6318499. doi: 
            10.1093/nar/gkab585.
  PUBMED    34244791
REFERENCE   2  (bases 1 to 8841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jul 9. pii: 6318499. doi: 10.1093/nar/gkab585."
COMMENT     Sequence Label: pLD-puro-Cn-VA-NHEJ1-S262E/S263E
FEATURES             Location/Qualifiers
     source          1..8841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             566..631
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             641..661
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             674..694
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             707..724
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             737..760
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             797..820
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    1855..1972
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2021..2531
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2450..2468
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2553..3152
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2553..2572)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3049..3069
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3280..3513
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3585..3706
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3622..3641)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3676..3695
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3746..3881
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3901..3920)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3902..3920)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3930..3947)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3930..3946)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3939..3961)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4088..4543
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4175..4194)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4385..4406
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4569..4673
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4674..4742,4743..5534)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4892..4911)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5705..6293
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6194..6213
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6447..6464
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6581..6602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6617..6622,6623..6640,6641..6647)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6655..6671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6660..6682
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6679..6695
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6679..6695
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6714..6734
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6716..6734
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6762..6988
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6989..7169
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7216..7341
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7834..8067
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8252..8296
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     8594..8614
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgtgctagca ccatggacta caaagaccat gacggtgatt ataaagatca
      601 tgacatcgat tacaaggatg acgatgacaa gcctgcagga gaaaatctgt acttccaggg
      661 gagaggaggc agcgagaacc tgtactttca ggggcctgca ggaagacatc accatcacca
      721 tcacagcggc ggaagatgga gccaccctca gttcgagaag ggaggaggaa gcggcggagg
      781 cagcggagga ggaagctgga gccacccgca gttcgagaaa gcgatcgcca gagagctgtt
      841 caacctgctg ggcgagaacc agccccccgt ggtgatcaag gcgatcgcca ccggtatgga
      901 agaactggag caaggcctgt tgatgcagcc atgggcgtgg ctacagcttg cagagaactc
      961 cctcttggcc aaggttttta tcaccaagca gggctatgcc ttgttggttt cagatcttca
     1021 acaggtgtgg catgaacagg tggacactag tgtggtcagc cagcgagcca aggagctgaa
     1081 caagcggctc actgctcctc ctgcagcttt cctctgtcat ttggataatc tccttcgccc
     1141 attgttgaag gacgctgctc accctagcga agctaccttc tcctgtgatt gtgtggcaga
     1201 tgcactgatt ctacgggtgc gaagtgagct ctctggcctc cccttctatt ggaatttcca
     1261 ctgcatgcta gctagtcctt ccctggtctc ccaacatttg attcgtcctc tgatgggcat
     1321 gagtctggca ttacagtgcc aagtgaggga gctagcaacg ttacttcata tgaaagacct
     1381 agagatccaa gactaccagg agagtggggc tacgctgatt cgagatcgat tgaagacaga
     1441 accatttgaa gaaaattcct tcttggaaca atttatgata gagaaactgc cagaggcatg
     1501 cagcattggt gatggaaagc cctttgtcat gaatctgcag gatctgtata tggcagtcac
     1561 cacacaagag gtccaagtgg gacagaagca tcaaggcgct ggagatcctc atacctcaaa
     1621 cagtgcttcc ctgcaaggaa tcgatagcca atgtgtaaac cagccagaac aactggtcga
     1681 ggaggcccca accctctcag cacctgagaa agagtccacg ggtacttcag gccctctgca
     1741 gagacctcag ctgtcaaagg tcaagaggaa gaagccaagg ggtctcttca gttaatctag
     1801 atggggtggc ttcgaattct cgacctcgag acaaatggca gtattcatcc acaattttaa
     1861 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     1921 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     1981 ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg ttgcgccttt
     2041 tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac
     2101 gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga
     2161 tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg
     2221 aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta
     2281 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg
     2341 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc
     2401 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat
     2461 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc
     2521 tctctcccca gggggatcca ccggagctta ccatgaccga gtacaagccc acggtgcgcc
     2581 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     2641 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     2701 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     2761 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     2821 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     2881 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     2941 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     3001 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     3061 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     3121 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
     3181 ccgaaaggag cgcacgaccc catgcatcgg tacctttaag accaatgact tacaaggcag
     3241 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     3301 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     3361 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3421 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3481 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     3541 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     3601 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3661 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     3721 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3781 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3841 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     3901 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     3961 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4021 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4081 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4141 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4201 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4261 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4321 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4381 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4441 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4501 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     4561 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4621 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4681 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4741 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4801 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4861 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4921 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4981 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5041 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5101 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5161 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5221 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5281 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5341 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5401 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5461 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5521 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5581 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5641 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5701 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5761 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5821 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5881 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5941 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6001 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6061 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6121 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6181 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6241 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6301 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6361 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6421 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6481 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     6541 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     6601 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     6661 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     6721 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     6781 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     6841 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     6901 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     6961 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     7021 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7081 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7141 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     7201 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     7261 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     7321 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     7381 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     7441 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     7501 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     7561 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     7621 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     7681 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     7741 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     7801 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     7861 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     7921 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     7981 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     8041 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     8101 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     8161 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     8221 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     8281 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     8341 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     8401 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     8461 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     8521 atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc ctcgagcggc
     8581 cgcccccttc accgagggcc tatttcccat gattccttca tatttgcata tacgatacaa
     8641 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
     8701 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
     8761 aatggactat catatgttga ctagttatta atagtaatca attacggggt cattagttca
     8821 tagcccatat atggagttcc g
//
