LOCUS       sequence-299733-       11930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insertion of split hygromycin landing pad into ChrII:8420157 site C.
            elegans strains via CRISPR with SEC selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11930)
  AUTHORS   Stevenson ZC, Moerdyk-Schauwecker MJ, Jamison B, Phillips PC
  TITLE     Rapid Self-Selecting and Clone-Free Integration of Transgenes into 
            Engineered CRISPR Safe Harbor Locations in Caenorhabditis elegans.
  JOURNAL   G3 (Bethesda). 2020 Aug 19. pii: g3.120.401400. doi: 
            10.1534/g3.120.401400.
  PUBMED    32816924
REFERENCE   2  (bases 1 to 11930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 19. pii: g3.120.401400. doi: 
            10.1534/g3.120.401400."
COMMENT     Sequence Label: pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)
FEATURES             Location/Qualifiers
     source          1..11930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(146..734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(226..245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(905..1696,1697..1765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1528..1547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1766..1870)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1938..1956
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1994..2016)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2116..2135
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    complement(4966..4999)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5009..5038
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(6855..7178)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    9096..9120
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9123..10148
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9123..10148
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(10149..10173)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10526..10559)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tgtgaaattg ttatccgctg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
       61 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag accaggaacc
      121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
      601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1021 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1861 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     1921 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     1981 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2041 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2101 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2161 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     2221 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2281 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agcacgtgtg tttttgaaca
     2341 cggcgatatg tatctgtaga tcatccccaa ttttcaacaa tgaatctaaa tactgaactt
     2401 tttgtgtaat ctttagtgca gaaaaatgac caaaatcagc gattttccga ttatacccaa
     2461 aatatctcag ctcaatgagc acctataaaa atgtaaaaat aaagaaagta gtcgaaccat
     2521 attttgtaaa ggctaaaaaa ctgaataaat ttttatccat ccagcgcggc gttacaatcc
     2581 gtggcccatg tcacgcacgg ctttcaaaga aaaaaaaaca gtctgtttgc gcgacatcga
     2641 tctggcaaaa atcctaaaaa cgactatgcg tggctcactt tatattaata tgaaaaatac
     2701 aaacttggtg gtgcaggtgc tgcagaggcc atcgatgaag aagtggaggc cttgttgtcg
     2761 atctacaaaa ttgtatggtt gtttgagatt ttccttgaaa cgctctcaat cttggatatg
     2821 atataaattc tggataagat atgatggata tgatctatgt ttgatagagt gcatttgaaa
     2881 ggataacggc cctgcagaca tttttgaaaa aataacaaac atttaatttt ctcactcgtt
     2941 taggctattc ccctattttt gatattcctt cgcacatatg aaaactactt tttttcgaaa
     3001 ctgttaactc cagaatttat aaatctatag cccttacttg attatttatt atcatggtta
     3061 ctcacctcat gtctctttct cttttcaaga agtcttcgtt catatctgtt gtctctttcc
     3121 tccttttgaa tctctgcttt gctcttcgcc attgtttcct gaaaataatg taacttgaat
     3181 tgtgtaatat acttttttaa tttgaatttg gcttgtaacg cggaatcact acgtgcggga
     3241 tcatttctta ctagaaaacc cagaaaatgc catatttcac ttatctcggg gtcatttcta
     3301 attagaaaag ctacaaaaac catttctaat tacggtgact ttagaactgg tcgaaatagc
     3361 taattagaaa tgggtttttg ctaattagag gtgactctat gttatctgag taagcctcgt
     3421 ttgtatgtaa actcacagta ctaataaatg caagacaccc gggtttgtct agatatgaaa
     3481 taattgaaat atcaattctg acagacaata atggtaatct tgataaggag ttccacgccc
     3541 aggagaacac gttagttttc ttgtttttga ttgcgtgcgt tattttggag aaaaactcga
     3601 ttttttacaa aataattttt tgaaaggaac actgttcaat aagttttgtc ttttttctca
     3661 gttgtgatac ggttttttat tcttttttgt agttatacag aagaccgcct gcaggggaca
     3721 gtcctgccga ggtggaggga ccgttacgtc taccgtcact tcgcctccgc cgccctccca
     3781 atcccagagg tcctcgacat cggagagttc tccgagtccc tcacctactg catctcccgt
     3841 cgtgcccaag gagtcaccct ccaagacctc ccagagaccg agctcccagc cgtcctccaa
     3901 ccagtcgccg aggccatgga cgccatcgcc gccgccgacc tctcccaaac ctccggattc
     3961 ggaccattcg gaccacaagg aatcggacaa tacaccacct ggcgtgactt catctgcgcc
     4021 atcgccgacc cacacgtcta ccactggcaa accgtcatgg acgacaccgt ctccgcctcc
     4081 gtcgcccaag ccctcgacga gctcatgctc tgggccgagg actgcccaga ggtccgtcac
     4141 ctcgtccacg ccgacttcgg atccaacaac gtcctcaccg acaacggacg tatcaccgcc
     4201 gtcatcgact ggtccgaggc catgttcgga gactcccaat acgaggtcgc caacatcttc
     4261 ttctggcgtc catggctcgc ctgcatggag caacaaaccc gttacttcga gcgtcgtcac
     4321 ccagagctcg ccggatcccc acgtctccgt gcctacatgc tccgtatcgg actcgaccaa
     4381 ctctaccaat ccctcgtcga cggaaacttc gacgacgccg cctgggccca aggacgttgc
     4441 gacgccatcg tccgttccgg agccggaacc gtcggacgta cccaaatcgc ccgtcgttcc
     4501 gccgccgtct ggaccgacgg atgcgtcgag gtcctcgccg actccggaaa ccgtcgtcca
     4561 tccacccgtc cacgtgccaa ggagtaagtc caattactct tcaacatccc tacatgctct
     4621 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt
     4681 tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga
     4741 attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc
     4801 tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat
     4861 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca
     4921 gtttatgacc gcaattttta ctagtgcagg agctgtaagt ttaaaataac ttcgtatagc
     4981 atacattata cgaagttatt ttcagggaga gcaaaagctt atctccgagg aggacctcta
     5041 aggatgatcg acgccaacgt cgttgaattt tcaaatttta aatactgaat atttgttttt
     5101 tttcctatta tttatttatt ctctttgtgt tttttttctt gctttctaaa aaattaattc
     5161 aatccaaatc taaacatttt tttttctctt tccgtctccc aattcgtatt ccgctcctct
     5221 catctgaaca caatgtgcaa gtttatttat cttctcgctt tcatttcatt aggacgtggg
     5281 gggaattggt ggaaggggga aacacacaaa aggatgatgg aaatgaaata aggacacaca
     5341 atatgcaaca acattcaatt cagaaatatg gaggaaggtt taaaagaaaa cataaaaata
     5401 tatagaggag gaaggaaact agctgccctc gattgtctat cttccattct caccaagttt
     5461 caacaacttt attcagtcat tcttctctgg ttcatttccc aaccaatctt cccgaataaa
     5521 gcccatctgt cctttcggcc acattcctca cctacatccg tactccttat ctcccgttct
     5581 tatccagtct tttatcgtct ctgcgtctcg cacactgtct cgtcggtaat gtaaaaaaaa
     5641 aacaaaaaaa acttgaaaac ttgcggaaag atttcctaat aaaaggagac gaatccggta
     5701 ggaaatcgac aagtctctgc ttctcgaaga ctaggtgagt tacatttctt tggccgaatt
     5761 attttgaaat cctaaatttg ataatttcag agatgtctgt aaaacttgcg tgttatgtga
     5821 cggcttcggt cactgtcgcc actcttatgg tatgcttcat gaccatgtca accatctact
     5881 cggaggttga tggattcaga gagaagcttg ataccgagat gaatgtgttc agagtgagtt
     5941 aaattatatt ttgaatttta aataattttt aattttctag caatctacca atggattgtg
     6001 gaaggacata gttgtcatcg gaagatctag caagcgtgtc cgttgtcaat atgaagagac
     6061 caacgctacc ccaactccac atgctgatgg atccccatct gctccaccag gtcaaccacc
     6121 agcagttcca ccagtcttca accagccaaa gactccaaat ggagccaatg gaaatggacc
     6181 aacctgcaac tgcaatgctg ataacaagtg cccagctgga ccatccggac caaagggagt
     6241 tccaggagtt ccaggactcg acggagttcc aggacttgac ggtgttccag gagttggagc
     6301 tgatgatatc gctccacaac gcgagtctgt cggatgcttc acttgcccac aaggaccagt
     6361 tggaccacca ggagctcttg gaagaccagg accacgtgga cttccaggac caagaggaca
     6421 aaatggaaac ccaggaagag atggacaacc aggacatcca ggagagcaag gatcatccgg
     6481 tcaaatcgga aagatcggag agccaggacc accaggagag aagggacgcg acgccgagca
     6541 tccaatcgga agaccaggac caaagggacc aagaggagat caaggaccaa caggaccagc
     6601 tggacagaac ggtcttcacg gaccaccagg agagccagga accgttggac cagaaggacc
     6661 atctggaaag caaggacgtc aaggaccaga cggaacccag ggagagactg gaccagacgg
     6721 aagaccagga aaggatgccg agtactgcca gtgcccagac aagtctccac catcagaggc
     6781 tgtcaacgcc aaccgtggat acagaaatat ctaaattgtt ggtgttttct aataaaatat
     6841 ttgagattca gtgagacttt tttcttggcg gcacaataaa gtttcctctt tttgttgggt
     6901 cactcaacga acacaataca aatatttgag cgctcgcatt tttctcaacc tttcgggttt
     6961 aaagcacttc aaaatggaat tgaagagaga ttgtgaaagt acatcagaca gaatcacatt
     7021 ttgatagcat tcacttcact cagatgcaaa cttttcagtg actataaaaa gtcataaaat
     7081 aataacatta aaaaagcaaa taaattagcg agagaatttt ttgacaaaaa gaaagaagag
     7141 tgatagagaa gaagggaatg cttgaaagga ttttgcattt atcttaatca ccgtcttcga
     7201 gaagacgaac catggcgcca gtttcagaat ccaagtttct aatatagttc atcacaatgt
     7261 tgacgttggt ccaacctccg gcttgcataa tctctgggat ggaaactcca gcgcgagcca
     7321 tgtcacgggc agctccgaca cgggcggaat gtccagacca agccaagtaa cgttgtccgg
     7381 aatcatcctt ggctccgtag atgagacggt gggtagcttc gaagattcct gaaaatttat
     7441 tttcagctta ttcttagcta atatgttgtt taaacttacc ttcaagagct cgagtcgaga
     7501 gttgtgatgt tgcactcggg gcagcaactc cgttcttgcg aacacgacag aacaggtagt
     7561 tgtttggatc atcggcgact ccggaaaccg aaatccagcg ctcaacgagc ttggtaactc
     7621 caagggagag cgctttttcg actccggcag tcgagaccag cgtttttgta cgtccgatgt
     7681 ggatgagcat tcttcctccg tctgtgcgac taatatcctt gacgcgaatg cgggcaattt
     7741 cggcgatacg gagaagtgtg ttgtaagcaa ttccgaggaa ggcgaggttt cggatgtcct
     7801 ggcaacgatc ggaattttcc ataagagaac ggacctgatc gaagtcggtt ctgaaaattt
     7861 cgtcaagtct taggattgaa tacattaata tttaaactta cctttcgaag gcgagggctt
     7921 gcttggctct ctctccagca tcaacatttt ccttgcggat acgtctcatt acgagagata
     7981 cggcattaga atctgatggg cgtggaagtc cagatcggcg atggagcata ttgagctgtc
     8041 cgagatgttg ttggatcgtc ttcaccgcca atccacgagc ttggaggtag agcaagtagt
     8101 cacgaacgtc ttctggctcg gctgggaacc acttgcggtt gttaagcttg caccaggcag
     8161 cccaagaacg gcacactgag agaagcatct tccaggtgtg ctcggagaag gcttgtctgt
     8221 cacggaacat atccatgagg ttcttgcgaa cttcatcact tgtcgcgtca actggaagtg
     8281 ctggaagatt ttgatgaaca gtgagaagat ttgacatatt ttcgaagttt tttagatgca
     8341 ctagaacaaa gcgtgttggc ttcctctgag cccgctttcc ttatataccc gcattctgca
     8401 gccttacaga atgttctaga aggtcctaga tgcattcgtt tgaaaatact cccggtgggt
     8461 gcaaagagac gcagacggaa aatgtatctg ggtctcttta ttgtgtacac tacttttcca
     8521 tgtaccgaat gtgagtcgcc ctccttttgc aacaagcagc tcgaatgttc tagaaaaagg
     8581 tggaaaatag tataaatacc gttgaaaata aataccgaac aacatttgct ctaattgtga
     8641 aattagaaat cttcaaacta taatcatgtc actttaccac tatttccgtc cagctcaacg
     8701 ttccgttttt ggtgatcatt tttgctttcg tcgtaaatct acacacgcgt ctcttccgtg
     8761 cgagagtcca agccagcagc caaattcgtt gactgagtat tcaacgttta tacgttgtcg
     8821 gcaacgagaa ataggaaaat gcatcgggaa atgttctttt ttcgattttt tccaaggttt
     8881 tgacaaattt taccacgaat tttgctatgt tttcaattaa aaaatatgtt attcaactgt
     8941 ttctatgagg aaaataaggc tttgcatgta attttcttat tcagcataat ttttaattaa
     9001 tttgaatttt ctgtcctaac gtttattttg ttttcttggt tatgactgat ctgaaattaa
     9061 tttttgaatt ttaaggtaat atgtcaggcg gtgccgcaag tttgtacaaa aaagcaggct
     9121 ccatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     9181 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     9241 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     9301 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     9361 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
     9421 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
     9481 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     9541 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     9601 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
     9661 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
     9721 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
     9781 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
     9841 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
     9901 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     9961 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
    10021 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
    10081 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
    10141 aggaatagac ccagctttct tgtacaaagt gggtccaatt actcttcaac atccctacat
    10201 gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat
    10261 ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa
    10321 atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat
    10381 aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga
    10441 taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg
    10501 tttcagttta tgaccgcaat ttttaataac ttcgtatagc atacattata cgaagttatt
    10561 ttcagggagc cggatctgag ctcttaagac gaagtgagtt tgctaccatc atggaatcag
    10621 gaggacattt cgacaatgtc atataatgtt tgaaaaatga ttttaacaag ggtctcattc
    10681 gagataagca atgctctgag caagtggctc gtcgtcttca agaatctctt gttgatattc
    10741 atttggatcc tgttttgcac gaagcatgtg ccatggatat tcagagtttt tgcaaaaaag
    10801 ttccgccagg tcaaacaatc tttttaattt ttatatgata cttcgggtgt atttttcttc
    10861 tttttcctgt tcattttcgc ttttttctca cttttttgta acaaaatctc tcacttttct
    10921 gtaagtctta acaaaagttc aattggctcg atcaagtaaa atttcggccg tatgaacgtt
    10981 ttcaaaggtt ttcatttttt tttaagttca ttcacaatat catgaatctc tcgtcctttg
    11041 gtttccggta agtagcgaaa taatataagc atacttataa atgaaggaat tgaatataga
    11101 ataaggaaag cataactacc aataacggaa tatagaggta atactgtaaa tgtactgata
    11161 accaccatta cagtattcat tgaatatgcc acagattgag caatactacg atgtttttga
    11221 ggtaccagtt cagaggatat aaaccacgaa attggaccaa ctccagttct gaaatttcat
    11281 tattcgatta aatcaaaata tatttgaaaa acctcaccca taagtataac cgtagatgat
    11341 caatgcaact aggcatccgt atttcatcgg gtcaatcaga atacgtattt ttgcgaagaa
    11401 tacgaatgca attagagcta aattattgag aaatgtgaag agaaggagaa gcttgcgtcg
    11461 accaactctg taaacttatt catacaattt tgtcttcaaa ttttgaacgt ttaaaactat
    11521 cttattcaat gagttagcgg gtttttatag tgagttagtt cacgattatc taataaaact
    11581 atgtccaaac atcctaactg cgaccgtatt ctcactaact cgtcttaaaa acgttgagat
    11641 tattttaaac ccttcttcag tacaactaca gtactgatgc acaaatacaa taataataat
    11701 acattcagac atactctgaa caatgtaact tgtttttgtt atactgtggt aacatttatt
    11761 gaaatgaaat ataaccttac ttttcaatca caattccacc agtaattgtt ccagaaacat
    11821 aagctaaacc cattgcagtt gtactccatt cagcaatttc tgactcaaca tttgcatttt
    11881 ctagaaagaa agtagaggat aaaagaagag cccataatcc gacagggccc
//
