LOCUS       sequence-29975-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Vassallo MF, Tanese N
  TITLE     Isoform-specific interaction of HP1 with human TAFII130.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Apr 30. 99(9):5919-24.
  PUBMED    11959914
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Apr 30. 99(9):5919-24."
COMMENT     Sequence Label: pcDNA HA HP1 gamma delta C
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             105..131
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(609..627)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(610..627)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(647..664)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    653..764
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgagc tcggatccac catgtaccca tacgatgttc
      121 cagattacgc tgaattcgga aaaaaacaga atggaaagag taaaaaagtt gaagaggcag
      181 agcctgaaga atttgtcgtg gaaaaagtac tagatcgacg tgtagtgaat gggaaagtgg
      241 aatatttcct gaagtggaag ggatttacag atgctgacaa tacttgggaa cctgaagaaa
      301 atttagattg tccagaattg attgaagcgt ttcttaactc tcagaaagct ggcaaagaaa
      361 aagatggtac aaaaagaaaa tctttatctg acagtgaatc tgatgacagc aaatcaaaga
      421 agaaaagaga tgctgctgac aaaccaagag gatttgccag aggtcttgat cctgaaagaa
      481 taattggtgc cacagacagc agtggagaat tgatgtttct catgaaatgg aaagattcag
      541 atgaggcaga cttggtgctg gcgaaagagg caaatatgta gctcgagcat gcatctagag
      601 ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct
      661 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
      721 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtag
//
