LOCUS       sequence-299765-        3705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To clone any gene or STTM sequence or artificial miRNA under CaMV 
            35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3705)
  TITLE     Cloning plasmid for STTMs and artificial miRNAs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOT2-poly-cis
FEATURES             Location/Qualifiers
     source          1..3705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..678)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKEITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     593..612
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(715..720,721..737,738..743))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(805..1394)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(885..904)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1498..1517
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1498..1516
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1924..2269
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2212..2232
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    2897..3073
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(3635..3653)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3636..3653)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tcgacaggcc tgtaccaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
       61 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
      121 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
      181 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
      241 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
      301 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
      361 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
      421 atcccatatt accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat
      481 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
      541 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
      601 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
      661 agtgatttct ttctccattt tagcttcctt agctcctgaa aatctcgata actcattaaa
      721 gcttatcgat gataagctgt caaacatgag aatttagccc gcctaatgag cgggcttttt
      781 gctgtgatcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      841 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      901 tcccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      961 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1021 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1081 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1141 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1201 ctacagagtt cttgatgtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1261 tctacgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1321 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1381 aaaaaggatc tcaaggagat cctttggatc acaaaaaaaa gcccgctcat taggcgggtt
     1441 aaattcccgg gtactgcagc cgattcatta atgcaggtta acctggctta tcgaaattaa
     1501 tacgactcac tatagggaga ccggtagatc tgatccctac tccaaaaatg tcaaagatac
     1561 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaattt cgggaaacct
     1621 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtgg aaaaggaagg
     1681 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atgcctctgc
     1741 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtgggaa aagaagacgt
     1801 tccaaccacg tcttcaaagc aagtggattg atgtgacatc tccactgacg taagggatga
     1861 cgcacaatcc cgcccctact ccaaaaatgt caaagatact gtctcagaag accaaagggc
     1921 tattgagact tttcaacaaa gggtaatttc gggaaacctc ctcggattcc attgcccagc
     1981 tacctgtcac ctcatcgaaa ggacagtaga aaaggaaggt ggctcctaca aatgccatca
     2041 tcgcgataaa ggaaaggcta tcattcaaga tgcctctgcc gacagtggtc ccaaagatgg
     2101 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
     2161 agtggattga tgtgacatct ccactgacat aagggatgac gcacaacccc actatccttc
     2221 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacag cccaagcttc
     2281 tctcttctct ttctccataa ccctgtctaa agggattatt atgatagtag aaaaaatgac
     2341 tctcgaaccc gttggcatgg gcccggcacc gtggtcgaga aataaaaaaa tcagagtggt
     2401 agcccgagcc atgcctagga gctccacatt ttcatatgac tgtgagctgg tgccacggat
     2461 cggttgccca agctgaataa aaaaaatgac actcctacgg gcacggatcg gtaccacgtg
     2521 gagattttga tatcactgtg aggatgcccg tgccatgcca tggtgcaccc ctaaaaaaaa
     2581 aaagtctcac catcgggctc ggatgtttaa atcggatcct cgaggtgtaa aaaaactcgt
     2641 aaatcctatc agatctggaa gatttctacg cttctccttc tttatattcg ttttcttatg
     2701 ctttttattt ttgatataac ctagaaaaag gttttttata tctttgaatc tgaaattgtt
     2761 tgttttagag tattgtatat ctgattttta tcccttttta tatttgaacg ttctttagtc
     2821 tccttttgtt tgcccaaatg ttgaattcgg tacgctgaaa tcaccagtct ctctctacaa
     2881 atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga taagggaaat
     2941 tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
     3001 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac
     3061 taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa ccagacacga
     3121 gacgactaga cctggagccc agacgccgtt cgaagctaga agtaccgctt aggcaggagg
     3181 ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt aggtttggtt
     3241 taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag gttgcaggcc
     3301 ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt atactatacg
     3361 ctaagggaat gattgtattt ataccctata ccccctaata accccttatc aatttaagaa
     3421 ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag gtctatgacc
     3481 aaaactcaag aggataaaac ctcaccaaaa tacgaaagag tccttaactc taaagataaa
     3541 agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc atgcgacctc
     3601 tagaccatca cattggagct tcagctgctc gagttctata gtgtcaccta aatcgtatgt
     3661 gtatgataca taaggttatg tattaattgt agccgcgttc taacg
//
