LOCUS       sequence-299766-        2853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of truncated ORF7B CDS 
            from SARS-CoV-2 genomic analysis in Kim et al. 2020.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 ORF7B C-trunc
FEATURES             Location/Qualifiers
     source          1..2853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     192..208
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     192..208
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     213..232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        213..231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    249..348
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(412..511)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(527..544)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(527..543)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(536..558)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      607..693
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      785..812
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(971..988)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1142..1730)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1222..1241)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1823..2614)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc cacggtgtgc
       61 gtcacccggc aaccttgggt agcagcgaag tcgaggcatt tctgtcctgg ctggtctaga
      121 ggaggcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacgg
      181 gccagagctg ccaggaaaca gctatgacca tgtaatacga ctcactatag gggatatcag
      241 ctggatggca aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
      301 ataagcaatg ctttcttata atgccaactt tgtacaagaa agttgggtat taagcgtggc
      361 aggtttcgtt gtggtcctgc agctccaggg agaaccagaa gatgatcagc atgccaactt
      421 ttttgtacaa agttggcatt ataaaaaagc attgctcatc aatttgttgc aacgaacagg
      481 tcactatcag tcaaaataaa atcattattt ggggcccgag cttaagactg gccgtcgttt
      541 tacaacgtcg tgactgggaa aacatccatg ctagcgttaa cgcgagagta gggaactgcc
      601 aggcatcaaa taaaacgaaa ggctcagtcg gaagactggg cctttcgttt tatctgttgt
      661 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgtg
      721 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaact
      781 aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct tcctggctag
      841 cggtacgcgt attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
      901 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
      961 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1021 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1081 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1141 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1201 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1261 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1321 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1381 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1441 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1501 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1561 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1621 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1681 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1741 tgatcttttc tacggggtct gacgctcagt ggaacgacgc gtaactcacg ttaagggatt
     1801 ttggtcatgg gtggctcgag ggttatttgc cgactacctt ggtgatctcg cctttcacgt
     1861 agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa
     1921 gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg
     1981 cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc
     2041 gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata
     2101 gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt
     2161 cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag
     2221 ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc
     2281 attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca
     2341 caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt
     2401 ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa
     2461 ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat
     2521 gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt
     2581 gagtcgatac ttcggcgatc accgcttccc tcataatgtt taactttgtt ttagggcgac
     2641 tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     2701 gcgcttgctg cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag
     2761 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
     2821 tgcgtgagcg catacgctac ttgcattaca gct
//
