LOCUS       sequence-299767-        9211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 NSP3 Cys857Ala
FEATURES             Location/Qualifiers
     source          1..9211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(62..148)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    201..300
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(6142..6241)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6353..6372)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(6431..6450)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(7024..7557)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7746..7751,7752..7768,7769..7774))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      8431..9019
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8920..8939
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(9154..9181)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
       61 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
      121 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
      181 ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg acctgttcgt
      241 tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagttggca
      301 tggcccctac caaggtgacc ttcggagacg acaccgtgat cgaggtgcag ggttacaaga
      361 gcgtgaacat caccttcgag ctggacgaac gcatcgacaa ggtgctgaac gaaaagtgct
      421 ccgcttacac cgtggagctg ggcaccgagg tgaacgaatt cgcctgcgtg gtggccgacg
      481 ctgtgatcaa gaccctgcag ccagtgtccg aactgctgac ccctctgggt atcgacctgg
      541 acgagtggtc tatggctacc tactacctgt tcgacgagag cggcgaattc aagctggcct
      601 ctcacatgta ctgcagcttc taccctcccg acgaagacga ggaagagggt gactgcgaag
      661 aggaagagtt cgagccttcc actcagtacg agtacggcac cgaagacgac taccagggca
      721 agcctctgga attcggagcc acctctgccg ctctgcagcc tgaagaggaa caggaggaag
      781 actggctgga cgacgactcc cagcagaccg tgggtcagca ggacggctct gaagacaacc
      841 agaccaccac catccagacc atcgtggagg tgcagcctca gctggagatg gaactgaccc
      901 cagtggtgca gaccatcgag gtgaacagct tctccggcta cctgaagctg accgacaacg
      961 tgtacatcaa gaacgctgac atcgtggagg aagccaagaa ggtgaaacca accgtggtgg
     1021 tgaacgccgc taacgtgtac ctgaagcacg gcggaggtgt ggctggagct ctgaacaagg
     1081 ctaccaacaa cgccatgcag gtggaatccg acgactacat cgctaccaac ggtcctctga
     1141 aggtgggcgg atcctgcgtg ctgtctggcc acaacctggc caagcactgc ctgcacgtgg
     1201 tgggaccaaa cgtgaacaag ggagaggaca tccagctgct gaagagcgcc tacgaaaact
     1261 tcaaccagca cgaggtgctg ctggctccac tgctgtctgc cggaatcttc ggtgctgatc
     1321 ccatccacag cctgcgcgtg tgcgtggaca ccgtgaggac caacgtgtac ctggccgtgt
     1381 tcgacaagaa cctgtacgac aagctggtga gctccttcct ggagatgaag tccgaaaagc
     1441 aggtggagca gaagatcgcc gagatcccca aggaggaagt gaagcctttc atcaccgaat
     1501 ctaagcccag cgtggagcag agaaagcagg acgacaagaa gatcaaggcc tgcgtggagg
     1561 aagtgaccac caccctggag gaaaccaagt tcctgaccga gaacctgctg ctgtacatcg
     1621 acatcaacgg caacctgcac cctgactccg ctaccctggt gtctgacatc gacatcacct
     1681 tcctgaagaa ggacgccccc tacatcgtgg gcgacgtggt gcaggaagga gtgctgaccg
     1741 ctgtggtcat ccctaccaag aaggccggtg gcaccaccga gatgctggcc aaggctctgc
     1801 gtaaggtgcc aaccgacaac tacatcacca cctaccccgg tcagggcctg aacggttaca
     1861 ccgtggagga agctaagacc gtgctgaaga agtgcaagtc tgccttctac atcctgccct
     1921 ccatcatctc taacgagaag caggaaatcc tgggcaccgt gagctggaac ctgcgcgaaa
     1981 tgctggccca cgctgaggaa accaggaagc tgatgcccgt gtgcgtggag accaaggcca
     2041 tcgtgagcac catccagaga aagtacaagg gcatcaagat ccaggagggt gtggtggact
     2101 acggcgctcg tttctacttc tacacctcca agaccaccgt ggcctctctg atcaacaccc
     2161 tgaacgacct gaacgagacc ctggtgacca tgccactggg atacgtgacc cacggtctga
     2221 acctggagga agccgctaga tacatgcgtt ccctgaaggt gcctgctacc gtgagcgtga
     2281 gcagccctga cgctgtgacc gcctacaacg gatacctgac ctcctctagc aagacccctg
     2341 aggaacactt catcgaaacc atcagcctgg ccggttccta caaggactgg tcttacagcg
     2401 gacagtccac ccagctgggc atcgagttcc tgaagcgcgg agacaagagc gtgtactaca
     2461 cctccaaccc aaccaccttc cacctggacg gtgaagtgat caccttcgac aacctgaaga
     2521 ccctgctgtc tctgcgcgag gtgaggacca tcaaggtgtt caccaccgtg gacaacatca
     2581 acctgcacac ccaggtggtg gacatgagca tgacctacgg acagcagttc ggtcctacct
     2641 acctggacgg agctgacgtg accaagatca agccacacaa ctcccacgag ggcaagacct
     2701 tctacgtgct gcctaacgat gacaccctga gagtggaggc tttcgaatac taccacacca
     2761 ccgacccatc cttcctgggc aggtacatgt ctgccctgaa ccacaccaag aagtggaagt
     2821 acccccaggt gaacggactg accagcatca agtgggccga caacaacgcc tacctggcca
     2881 ccgctctgct gaccctgcag cagatcgaac tgaagttcaa cccacccgct ctgcaggacg
     2941 cttactacag agcccgtgct ggcgaggccg ctaacttctg cgctctgatc ctggcctact
     3001 gcaacaagac cgtgggcgaa ctgggagacg tgagagagac catgagctac ctgttccagc
     3061 acgccaacct ggactcctgc aagcgtgtgc tgaacgtggt gtgcaagacc tgcggccagc
     3121 agcagaccac cctgaaggga gtggaagctg tgatgtacat gggtaccctg tcctacgagc
     3181 agttcaagaa gggcgtgcag atcccttgca cctgcggaaa gcaggccacc aagtacctgg
     3241 tgcagcagga aagcccattc gtgatgatgt ccgctcctcc agcccagtac gaactgaagc
     3301 acggcacctt cacctgcgct tccgagtaca ccggcaacta ccagtgcgga cactacaagc
     3361 acatcacctc taaggagacc ctgtactgca tcgacggagc cctgctgacc aagtcctctg
     3421 aatacaaggg tcccatcacc gacgtgttct acaaggagaa cagctacacc accaccatca
     3481 agcccgtgac ctacaagctg gacggcgtgg tgtgcaccga aatcgaccct aagctggaca
     3541 actactacaa gaaggacaac tcttacttca ccgagcagcc tatcgacctg gtgccaaacc
     3601 agccctaccc taacgccagc ttcgacaact tcaagttcgt gtgcgacaac atcaagttcg
     3661 ctgacgacct gaaccagctg accggataca agaagcctgc ctctagagag ctgaaggtga
     3721 ccttcttccc agacctgaac ggcgacgtgg tggctatcga ctacaagcac tacaccccaa
     3781 gcttcaagaa gggagccaag ctgctgcaca agcccatcgt gtggcacgtg aacaacgcta
     3841 ccaacaaggc cacctacaag cctaacacct ggtgcatccg ttgcctgtgg tctaccaagc
     3901 cagtggaaac ctccaactct ttcgacgtgc tgaagagcga ggacgctcag ggcatggaca
     3961 acctggcctg cgaagacctg aagcccgtga gcgaggaagt ggtggagaac ccaaccatcc
     4021 agaaggacgt gctggaatgc aacgtgaaga ccaccgaggt ggtgggagac atcatcctga
     4081 agccagctaa caacagcctg aagatcaccg aggaagtggg tcacaccgac ctgatggccg
     4141 cttacgtgga caacagctcc ctgaccatca agaagcccaa cgagctgagc agggtgctgg
     4201 gtctgaagac cctggctact cacggtctgg ctgctgtgaa ctctgtgcca tgggacacca
     4261 tcgctaacta cgccaagcct ttcctgaaca aggtggtgtc caccaccacc aacatcgtga
     4321 ccagatgtct gaaccgcgtg tgcaccaact acatgcccta cttcttcacc ctgctgctgc
     4381 agctgtgcac cttcaccaga agcaccaact cccgtatcaa ggctagcatg cctaccacca
     4441 tcgccaagaa caccgtgaag agcgtgggaa agttctgcct ggaggcttcc ttcaactacc
     4501 tgaagtctcc aaacttcagc aagctgatca acatcatcat ctggttcctg ctgctctccg
     4561 tgtgcctggg ttccctgatc tactctaccg ccgctctggg agtgctgatg tctaacctgg
     4621 gtatgcctag ctactgcact ggttacagag agggatacct gaacagcacc aacgtgacca
     4681 tcgccaccta ctgcaccggc agcatcccat gctccgtgtg cctgtctgga ctggacagcc
     4741 tggacaccta cccctccctg gaaaccatcc agatcaccat ctctagcttc aagtgggacc
     4801 tgaccgcctt cggtctggtg gctgagtggt tcctggccta catcctgttc accaggttct
     4861 tctacgtgct gggcctggcc gctatcatgc agctgttctt ctcctacttc gctgtgcact
     4921 tcatcagcaa ctcctggctg atgtggctga tcatcaacct ggtgcagatg gctcccatct
     4981 ctgccatggt gcgcatgtac atcttcttcg ccagcttcta ctacgtgtgg aagtcctacg
     5041 tgcacgtggt ggacggttgc aactcctcta cctgcatgat gtgctacaag cgcaacaggg
     5101 ctaccagggt ggagtgcacc accatcgtga acggcgtgcg caggtccttc tacgtgtacg
     5161 ccaacggcgg caagggattc tgcaagctgc acaactggaa ctgcgtgaac tgcgacacct
     5221 tctgcgctgg ctccaccttc atctctgacg aggtggccag agacctgagc ctgcagttca
     5281 agcgtccaat caaccccacc gaccagagct cctacatcgt ggactctgtg accgtgaaga
     5341 acggtagcat ccacctgtac ttcgacaagg ctggccagaa gacctacgag cgccactctc
     5401 tgagccactt cgtgaacctg gacaacctga gggccaacaa caccaagggc agcctgccta
     5461 tcaacgtgat cgtgttcgac ggaaagtcca agtgcgagga atctagcgcc aagtccgcca
     5521 gcgtgtacta cagccagctg atgtgccagc caatcctgct gctggaccag gctctggtgt
     5581 ctgacgtggg agacagcgcc gaagtggccg tgaagatgtt cgacgcctac gtgaacacct
     5641 tctcctctac cttcaacgtg cctatggaga agctgaagac cctggtggct actgctgagg
     5701 ctgaactggc taagaacgtg tccctggaca acgtgctgag caccttcatc tccgccgcta
     5761 ggcagggttt cgtggactct gacgtggaaa ccaaagacgt ggtggagtgc ctgaagctgt
     5821 ctcaccagag cgacatcgaa gtgaccggcg actcctgcaa caactacatg ctgacctaca
     5881 acaaggtgga gaacatgacc ccaagagacc tgggagcttg catcgactgc tccgcccgtc
     5941 acatcaacgc tcaggtggcc aagtctcaca acatcgccct gatctggaac gtgaaggact
     6001 tcatgagcct gtccgagcag ctgcgcaagc agatcaggag cgccgctaag aagaacaacc
     6061 tgcccttcaa gctgacctgc gctaccacca gacaggtggt gaacgtggtg accaccaaga
     6121 tcgccctgaa gggcggataa tacccaactt tcttgtacaa agttggcatt ataagaaagc
     6181 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     6241 gccatccagc tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     6301 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     6361 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     6421 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     6481 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     6541 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     6601 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     6661 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     6721 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     6781 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     6841 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     6901 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     6961 gataacctta tttttgacga ggggaaatta ggccgggaag ccgatctcgg cttgaacgaa
     7021 ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa
     7081 ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata
     7141 agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg
     7201 cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc
     7261 aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc
     7321 aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg
     7381 ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat
     7441 ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc
     7501 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt
     7561 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
     7621 gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt
     7681 taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc
     7741 attacagttt acgaaccgaa caggcttatg tcaactgggt tcgtgccttc atccgtttcc
     7801 acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc
     7861 tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt
     7921 tcttctacgg caaggtgctg tgcacggatc tgccctggct tcaggagatc ggaagacctc
     7981 ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc atcctcggtt
     8041 ttctggaagg cgagcatcgt ttgttcgccc aggactctag ctatagttct agtggttggc
     8101 tactaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     8161 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     8221 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     8281 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac
     8341 gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8401 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8461 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8521 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8581 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8641 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8701 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8761 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8821 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8881 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8941 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9001 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9061 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9121 taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc
     9181 tgcttagttt gatgcctggc agtttatggc g
//
