LOCUS       sequence-299773-        9208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 NSP3 Cys857Ala_nostop
FEATURES             Location/Qualifiers
     source          1..9208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(84..111)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(203..289)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    342..441
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(6280..6379)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6491..6510)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(6569..6588)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(7162..7695)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7884..7889,7890..7906,7907..7912))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      8569..9157
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9058..9077
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgctagc
       61 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      121 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      181 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcatc gacaaacaac
      241 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
      301 ttccctactc tcgcgttaac gctagcatgg atctcgggcc ccaaataatg attttatttt
      361 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
      421 ctttgtacaa aaaagttggc atggccccta ccaaggtgac cttcggagac gacaccgtga
      481 tcgaggtgca gggttacaag agcgtgaaca tcaccttcga gctggacgaa cgcatcgaca
      541 aggtgctgaa cgaaaagtgc tccgcttaca ccgtggagct gggcaccgag gtgaacgaat
      601 tcgcctgcgt ggtggccgac gctgtgatca agaccctgca gccagtgtcc gaactgctga
      661 cccctctggg tatcgacctg gacgagtggt ctatggctac ctactacctg ttcgacgaga
      721 gcggcgaatt caagctggcc tctcacatgt actgcagctt ctaccctccc gacgaagacg
      781 aggaagaggg tgactgcgaa gaggaagagt tcgagccttc cactcagtac gagtacggca
      841 ccgaagacga ctaccagggc aagcctctgg aattcggagc cacctctgcc gctctgcagc
      901 ctgaagagga acaggaggaa gactggctgg acgacgactc ccagcagacc gtgggtcagc
      961 aggacggctc tgaagacaac cagaccacca ccatccagac catcgtggag gtgcagcctc
     1021 agctggagat ggaactgacc ccagtggtgc agaccatcga ggtgaacagc ttctccggct
     1081 acctgaagct gaccgacaac gtgtacatca agaacgctga catcgtggag gaagccaaga
     1141 aggtgaaacc aaccgtggtg gtgaacgccg ctaacgtgta cctgaagcac ggcggaggtg
     1201 tggctggagc tctgaacaag gctaccaaca acgccatgca ggtggaatcc gacgactaca
     1261 tcgctaccaa cggtcctctg aaggtgggcg gatcctgcgt gctgtctggc cacaacctgg
     1321 ccaagcactg cctgcacgtg gtgggaccaa acgtgaacaa gggagaggac atccagctgc
     1381 tgaagagcgc ctacgaaaac ttcaaccagc acgaggtgct gctggctcca ctgctgtctg
     1441 ccggaatctt cggtgctgat cccatccaca gcctgcgcgt gtgcgtggac accgtgagga
     1501 ccaacgtgta cctggccgtg ttcgacaaga acctgtacga caagctggtg agctccttcc
     1561 tggagatgaa gtccgaaaag caggtggagc agaagatcgc cgagatcccc aaggaggaag
     1621 tgaagccttt catcaccgaa tctaagccca gcgtggagca gagaaagcag gacgacaaga
     1681 agatcaaggc ctgcgtggag gaagtgacca ccaccctgga ggaaaccaag ttcctgaccg
     1741 agaacctgct gctgtacatc gacatcaacg gcaacctgca ccctgactcc gctaccctgg
     1801 tgtctgacat cgacatcacc ttcctgaaga aggacgcccc ctacatcgtg ggcgacgtgg
     1861 tgcaggaagg agtgctgacc gctgtggtca tccctaccaa gaaggccggt ggcaccaccg
     1921 agatgctggc caaggctctg cgtaaggtgc caaccgacaa ctacatcacc acctaccccg
     1981 gtcagggcct gaacggttac accgtggagg aagctaagac cgtgctgaag aagtgcaagt
     2041 ctgccttcta catcctgccc tccatcatct ctaacgagaa gcaggaaatc ctgggcaccg
     2101 tgagctggaa cctgcgcgaa atgctggccc acgctgagga aaccaggaag ctgatgcccg
     2161 tgtgcgtgga gaccaaggcc atcgtgagca ccatccagag aaagtacaag ggcatcaaga
     2221 tccaggaggg tgtggtggac tacggcgctc gtttctactt ctacacctcc aagaccaccg
     2281 tggcctctct gatcaacacc ctgaacgacc tgaacgagac cctggtgacc atgccactgg
     2341 gatacgtgac ccacggtctg aacctggagg aagccgctag atacatgcgt tccctgaagg
     2401 tgcctgctac cgtgagcgtg agcagccctg acgctgtgac cgcctacaac ggatacctga
     2461 cctcctctag caagacccct gaggaacact tcatcgaaac catcagcctg gccggttcct
     2521 acaaggactg gtcttacagc ggacagtcca cccagctggg catcgagttc ctgaagcgcg
     2581 gagacaagag cgtgtactac acctccaacc caaccacctt ccacctggac ggtgaagtga
     2641 tcaccttcga caacctgaag accctgctgt ctctgcgcga ggtgaggacc atcaaggtgt
     2701 tcaccaccgt ggacaacatc aacctgcaca cccaggtggt ggacatgagc atgacctacg
     2761 gacagcagtt cggtcctacc tacctggacg gagctgacgt gaccaagatc aagccacaca
     2821 actcccacga gggcaagacc ttctacgtgc tgcctaacga tgacaccctg agagtggagg
     2881 ctttcgaata ctaccacacc accgacccat ccttcctggg caggtacatg tctgccctga
     2941 accacaccaa gaagtggaag tacccccagg tgaacggact gaccagcatc aagtgggccg
     3001 acaacaacgc ctacctggcc accgctctgc tgaccctgca gcagatcgaa ctgaagttca
     3061 acccacccgc tctgcaggac gcttactaca gagcccgtgc tggcgaggcc gctaacttct
     3121 gcgctctgat cctggcctac tgcaacaaga ccgtgggcga actgggagac gtgagagaga
     3181 ccatgagcta cctgttccag cacgccaacc tggactcctg caagcgtgtg ctgaacgtgg
     3241 tgtgcaagac ctgcggccag cagcagacca ccctgaaggg agtggaagct gtgatgtaca
     3301 tgggtaccct gtcctacgag cagttcaaga agggcgtgca gatcccttgc acctgcggaa
     3361 agcaggccac caagtacctg gtgcagcagg aaagcccatt cgtgatgatg tccgctcctc
     3421 cagcccagta cgaactgaag cacggcacct tcacctgcgc ttccgagtac accggcaact
     3481 accagtgcgg acactacaag cacatcacct ctaaggagac cctgtactgc atcgacggag
     3541 ccctgctgac caagtcctct gaatacaagg gtcccatcac cgacgtgttc tacaaggaga
     3601 acagctacac caccaccatc aagcccgtga cctacaagct ggacggcgtg gtgtgcaccg
     3661 aaatcgaccc taagctggac aactactaca agaaggacaa ctcttacttc accgagcagc
     3721 ctatcgacct ggtgccaaac cagccctacc ctaacgccag cttcgacaac ttcaagttcg
     3781 tgtgcgacaa catcaagttc gctgacgacc tgaaccagct gaccggatac aagaagcctg
     3841 cctctagaga gctgaaggtg accttcttcc cagacctgaa cggcgacgtg gtggctatcg
     3901 actacaagca ctacacccca agcttcaaga agggagccaa gctgctgcac aagcccatcg
     3961 tgtggcacgt gaacaacgct accaacaagg ccacctacaa gcctaacacc tggtgcatcc
     4021 gttgcctgtg gtctaccaag ccagtggaaa cctccaactc tttcgacgtg ctgaagagcg
     4081 aggacgctca gggcatggac aacctggcct gcgaagacct gaagcccgtg agcgaggaag
     4141 tggtggagaa cccaaccatc cagaaggacg tgctggaatg caacgtgaag accaccgagg
     4201 tggtgggaga catcatcctg aagccagcta acaacagcct gaagatcacc gaggaagtgg
     4261 gtcacaccga cctgatggcc gcttacgtgg acaacagctc cctgaccatc aagaagccca
     4321 acgagctgag cagggtgctg ggtctgaaga ccctggctac tcacggtctg gctgctgtga
     4381 actctgtgcc atgggacacc atcgctaact acgccaagcc tttcctgaac aaggtggtgt
     4441 ccaccaccac caacatcgtg accagatgtc tgaaccgcgt gtgcaccaac tacatgccct
     4501 acttcttcac cctgctgctg cagctgtgca ccttcaccag aagcaccaac tcccgtatca
     4561 aggctagcat gcctaccacc atcgccaaga acaccgtgaa gagcgtggga aagttctgcc
     4621 tggaggcttc cttcaactac ctgaagtctc caaacttcag caagctgatc aacatcatca
     4681 tctggttcct gctgctctcc gtgtgcctgg gttccctgat ctactctacc gccgctctgg
     4741 gagtgctgat gtctaacctg ggtatgccta gctactgcac tggttacaga gagggatacc
     4801 tgaacagcac caacgtgacc atcgccacct actgcaccgg cagcatccca tgctccgtgt
     4861 gcctgtctgg actggacagc ctggacacct acccctccct ggaaaccatc cagatcacca
     4921 tctctagctt caagtgggac ctgaccgcct tcggtctggt ggctgagtgg ttcctggcct
     4981 acatcctgtt caccaggttc ttctacgtgc tgggcctggc cgctatcatg cagctgttct
     5041 tctcctactt cgctgtgcac ttcatcagca actcctggct gatgtggctg atcatcaacc
     5101 tggtgcagat ggctcccatc tctgccatgg tgcgcatgta catcttcttc gccagcttct
     5161 actacgtgtg gaagtcctac gtgcacgtgg tggacggttg caactcctct acctgcatga
     5221 tgtgctacaa gcgcaacagg gctaccaggg tggagtgcac caccatcgtg aacggcgtgc
     5281 gcaggtcctt ctacgtgtac gccaacggcg gcaagggatt ctgcaagctg cacaactgga
     5341 actgcgtgaa ctgcgacacc ttctgcgctg gctccacctt catctctgac gaggtggcca
     5401 gagacctgag cctgcagttc aagcgtccaa tcaaccccac cgaccagagc tcctacatcg
     5461 tggactctgt gaccgtgaag aacggtagca tccacctgta cttcgacaag gctggccaga
     5521 agacctacga gcgccactct ctgagccact tcgtgaacct ggacaacctg agggccaaca
     5581 acaccaaggg cagcctgcct atcaacgtga tcgtgttcga cggaaagtcc aagtgcgagg
     5641 aatctagcgc caagtccgcc agcgtgtact acagccagct gatgtgccag ccaatcctgc
     5701 tgctggacca ggctctggtg tctgacgtgg gagacagcgc cgaagtggcc gtgaagatgt
     5761 tcgacgccta cgtgaacacc ttctcctcta ccttcaacgt gcctatggag aagctgaaga
     5821 ccctggtggc tactgctgag gctgaactgg ctaagaacgt gtccctggac aacgtgctga
     5881 gcaccttcat ctccgccgct aggcagggtt tcgtggactc tgacgtggaa accaaagacg
     5941 tggtggagtg cctgaagctg tctcaccaga gcgacatcga agtgaccggc gactcctgca
     6001 acaactacat gctgacctac aacaaggtgg agaacatgac cccaagagac ctgggagctt
     6061 gcatcgactg ctccgcccgt cacatcaacg ctcaggtggc caagtctcac aacatcgccc
     6121 tgatctggaa cgtgaaggac ttcatgagcc tgtccgagca gctgcgcaag cagatcagga
     6181 gcgccgctaa gaagaacaac ctgcccttca agctgacctg cgctaccacc agacaggtgg
     6241 tgaacgtggt gaccaccaag atcgccctga agggcggata cccaactttc ttgtacaaag
     6301 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     6361 aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca aaatctctga
     6421 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     6481 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     6541 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     6601 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     6661 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     6721 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     6781 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     6841 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     6901 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     6961 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     7021 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     7081 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattagg ccgggaagcc
     7141 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     7201 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     7261 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     7321 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     7381 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     7441 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     7501 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     7561 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     7621 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     7681 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     7741 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     7801 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     7861 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     7921 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     7981 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     8041 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     8101 aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     8161 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     8221 atagttctag tggttggcta ctaataggtt gtattgatgt tggacgagtc ggaatcgcag
     8281 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     8341 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     8401 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga
     8461 gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt
     8521 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8581 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8641 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8701 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8761 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8821 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8881 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8941 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9001 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9061 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9121 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9181 acggttcctg gccttttgct ggcctttt
//
