LOCUS       sequence-299787-        2308 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human synapsin-1 promoter; Flp-dependent rM3D(Gs) DREADD-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2308)
  TITLE     Unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSyn-fDIO-rM3D(Gs)-mCherry-WPREpA
FEATURES             Location/Qualifiers
     source          1..2308
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    32..65
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(158..868)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(436..455)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     705..723
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      865..874
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2138..2171)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 aggtaccgga tcctctagag tcgactccgg agaagttcct attctctaga aagtatagga
       61 acttcgcaga atggtagctg gattgtagct gctattagca atatgaaacc tcttagaagt
      121 tcctattctt caaaaggtat aggaacttcg gcgcgcctta cttgtacagc tcgtccatgc
      181 cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt
      241 tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc tgcacgggct
      301 tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca
      361 gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg
      421 cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag ttggtgccgc
      481 gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctgg gtcacggtca
      541 ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca
      601 gcttcaagta gtcggggatg tcggcggggt gcttcacgta ggccttggag ccgtacatga
      661 actgagggga caggatgtcc caggcgaagg gcagggggcc acccttggtc accttcagct
      721 tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt
      781 ggccgttcac ggagccctcc atgtgcacct tgaagcgcat gaactccttg atgatggcca
      841 tgttatcctc ctcgcccttg ctcaccatgg tggcgaccgg tggatccgcc aaggcctgct
      901 ccggcactcg cttgtgaaaa atgaccgact gtctctgctg gtactgctgt ttgcgcctct
      961 tccttttgtc acactggcac aagaggagcg tcttgaaggt ggttctgaat gttttgttgc
     1021 acagggcata gcacacaggg ttcacggtgc tgttgatata gcacagccag tagcccagat
     1081 tccaataggt tttgggtatg cagctgtcac agaaggtgtt caccaggacc atgatgttgt
     1141 agggggtcca cgtgatgatg aaggccagca agatggcact gagcgtctgc agagctttgt
     1201 gttccctcat ggccatgaca cgggacgtct tcctcttgct ggcacggccg ttgccgagga
     1261 tgggctggtg ttgggggagc gggggtggct gcggctgctc ctggctgcca tagaaccggc
     1321 cctcgcagcg gtcgatcttc ctgatctgct ccttggcctc ccggtacacc cgccagtata
     1381 aaatagtcat gatggtgaca ggcatgtaaa agccagcgat cgccgtgccg aaggtgatgg
     1441 tgggctcact cagaaactga atgaaacatt ctcctggggg cacagttctc ttccctacaa
     1501 agtattgcca gaacaagatg gcaggagccc ataggacaaa ggagatgacc caagccagac
     1561 caatcatcac accagctcga gccctggtca tcaggctctg gtagcggaat ggagaagtga
     1621 tggaaaagta cctgtcaaag ctgatgacca gcagattcat gacagaggca ttgctggcca
     1681 cacagtcaat ggagagccag aggtcgcagg ctaagttccc cagtgcccaa cggttcataa
     1741 tgatgtaggt agtgaacagg ttcatggaaa tgaccccgat gatcaggtct gcacaggcca
     1801 ggcttaagag gaagtagttg ttgactgtct tcagctgttt gttgaccttg aaggccacaa
     1861 tgacaaggat gttgccaatg atggtcacca atgccaggaa gccagttaag aaggcaatga
     1921 agaccacttg ccagatggtg tgacccccga gagggtcgct ggaggtgtcg tttgaggaga
     1981 aattcccagt ttcttgtgaa atgttgtagc tgcccaactg agtgactgtc cccaagggca
     2041 gccctgcctc cgagggactg tgcacccagg aagagctgat gttgggaaac aaaggcgagg
     2101 ttgtactgtt actgtgcaag gtcatggtgg cgctagcgaa gttcctatac tttctagaga
     2161 ataggaactt cttgccttaa cccagaaatt atcactgtta ttctttagaa tggtgcaaag
     2221 agaagttcct ataccttttg aagaatagga acttcgaatt cgatatcaag cttatcgata
     2281 atcaacctct ggattacaaa atttgtga
//
