LOCUS       sequence-299788-        2502 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2502)
  TITLE     Unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA
FEATURES             Location/Qualifiers
     source          1..2502
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..711)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(279..298)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     548..566
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      708..717
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg
       61 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta
      121 ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta
      181 gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc
      241 ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc
      301 gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag
      361 ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca
      421 cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac
      481 gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg
      541 gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc
      601 ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg
      661 catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca tggtggcgac
      721 cgggggatcc ttcaaggcct gctcgggtgc gcgcttgtga aaaatgaccg actgtctctg
      781 ctggtactgc tgcttgcgcc tctttttttt gtcacactgg cacagcagca gcatcttgaa
      841 agtggttctg aatgttttgt tgcacagagc atagcacacg gggttcacgg tgctgttgat
      901 gtagcacagc cagtagccca gattccaaaa ggttttgggt atgcagctgt cacaaaaggt
      961 gttcaccaga accatgatgt tgtatggggt ccaagtgatg atgaaggcaa gcaagatcgc
     1021 actgagggtc tgggccgctt tcttctcctt gaccagggac atccttttcc gcttagtgat
     1081 ctgacttctg gtcttcagag caaacctctt ggccagagtg gcttccttga aggacagagg
     1141 tagagtggcc gtgctcttac ccactgagga gttgacgtca gaagtcttag ctgtgtccac
     1201 ggctgactct agctggatgg gaagcttgga gaagcttttt ggaaaactgc ctccatcgtc
     1261 cacgctcttc tgggcctgca gcttgtcggc tttcctctcc aagtccacca tccccagctc
     1321 ctcctcaggc acctgcaggt tgtccgatga gggtaacttg gtggagttga ggatggtgct
     1381 gtgacccgga agcttgagca cgatggagta gatggctctc gtctcggagc caatgtcctc
     1441 ctcgtcggag gaggcggagt tctccaggga ggcagcagca tcattgttgt tccaactgtc
     1501 actgctgctg tggtcttggt ccatctgctc ggagctgggt ttccagctct tggttgtgaa
     1561 ccagaagtgg cagcggccat acttcctcct gttggagcgt ttcatgcttt gctgttgaag
     1621 ttcgtaactg ctgcagcttc gagaactgcc cgtggggtgg acaaagtttt ctgtctctgc
     1681 ctctgtccca gaggcttgca ggccagcaag ctctttggta cgcttttcag tttccttata
     1741 gatcctccag tataaaatag tcataatggt gacaggcata taaaaaccag cgatggctgt
     1801 gccaaaagta atggtgggct cactgaggaa ctgaatgaag cactctcccg gaggcacagt
     1861 tctctttcca acaaagtatt gccagaacaa gatggcagga gcccaaagga caaaggagat
     1921 gacccaagcc agaccgatca tcacaccggc tctctttgtt gttcgtttgg ctcggtacgt
     1981 gagcggcctc gtgatggaaa agtatctgtc aaagctgatg accagaagat tcataacaga
     2041 ggcattgctg gctacgcagt caatggcaag ccagaggtca caggccaagt tccctaaggc
     2101 ccatcgattc atgatgatgt aggtcgtaaa cagattcatt gaaatgaccc cgataatcag
     2161 atcggcacag gccaggctta agaggaagta gttgttgacc gtcttcagct gcttgttgac
     2221 cttaaatgac acaattacca ggatgttgcc gatgatggtc accaaggcca ggatgcccgt
     2281 taagaaagcg atgaagacca cttgccagac ggtatgacct cccagagggt catcggtggt
     2341 accgtctgga gaggagaaat tgccagctgc tcgagaaaca ttgtagctgc cgaaatgagt
     2401 gacggttccc gggggcagcc ctgcatcgga ggggctgtgt atccaggagg agctgatgtt
     2461 tggaaacaaa ggcgaggttg tactgttatt gtgcaaggtc at
//
