LOCUS       sequence-299789-        2595 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flp-dependent hM4D(Gi) DREADD-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Runegaard AH, Sorensen AT, Fitzpatrick CM, Jorgensen SH, Petersen 
            AV, Hansen NW, Weikop P, Andreasen JT, Mikkelsen JD, Perrier JF, 
            Woldbye D, Rickhag M, Wortwein G, Gether U
  TITLE     Locomotor- and Reward-Enhancing Effects of Cocaine Are 
            Differentially Regulated by Chemogenetic Stimulation of Gi-Signaling
            in Dopaminergic Neurons.
  JOURNAL   eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-0345-17. doi: 
            10.1523/ENEURO.0345-17.2018. eCollection 2018 May-Jun.
  PUBMED    29938215
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eNeuro."; 
            date: "2018-06-18"; volume: "5(3). pii: eN-NWR-0345-17. doi"; pages:
            " 10.1523/ENEURO.0345-17.2018. eCollection 2018 May-Jun"
COMMENT     Sequence Label: pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA
FEATURES             Location/Qualifiers
     source          1..2595
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..100
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(193..903)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(471..490)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     740..758
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      900..909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2358..2367
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2374..2407)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(2569..2589)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gaggagtcgt gtcgtgcctg agagcgcagt cgagaaggta ccggatcctc tagagtcgac
       61 tccggagaag ttcctattct ctagaaagta taggaacttc gcagaatggt agctggattg
      121 tagctgctat tagcaatatg aaacctctta gaagttccta ttcttcaaaa ggtataggaa
      181 cttcggcgcg ccttacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc
      241 gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat
      301 gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac
      361 ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag
      421 ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg
      481 cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc
      541 gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat
      601 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc
      661 ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc
      721 gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg
      781 gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg
      841 caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac
      901 catggtggcg accggtggat cccgcctggc agtgccgatg ttccgatact ggcacagcag
      961 caggtgccgg aaggtctttt taaaggtggc gttgcacaga gcatagcagg cagggttgat
     1021 ggtgctgttg acgtagcaga gccagtagcc aatggaccac accgtgtcag ggatgcagct
     1081 ctggcagaag gtgttcacca ggaccatgac gttgtagggc gtccaggtga ggatgaaggc
     1141 cagcagaatg gcaaagatcg ttcgtgtcac tttgcgctcc cgggccgcca tctgccgctt
     1201 cttgcgcacc tggttgcgag cgatgctggc gaacttgcgg gccacgttgg ccgcagggcg
     1261 catgccagcc ggcgtggcag gcacaatctc aatggctgtc acacactcat tgcctgtctg
     1321 cttcgtcaca atctggatct tggaccatct ggaggctggg ttgagggccc gcggctgcag
     1381 gggaggggcg ggcatggcgg gcgtggtggc ctctgtggtg gacagctctg tggctgggcg
     1441 ttccttggtg ttctgggtgg cactgcctga gctggactca ttggaagtgt ccttatcagc
     1501 cacggggcgc ggtggcggtg gcagcgctgg cgggggggcc tcctccagct tgccattgcg
     1561 cagctcctcc cgggcggcct ccccgggcgg gggcttcttg acgctctgct tcattagtgg
     1621 gctcttgagg aaggccagcg tcttggcttt cttctccttc gggccctcgg gccggtgctt
     1681 gtggactcgg ctgcgactgg ccagggagat gtggatgtac agcaccgtca tgatgaccac
     1741 aggcaggtag aagccagcaa tggctgtgcc aaaggtcact gctgggttgg acaggaactg
     1801 gatgaagcac tggttgtcgg gcaccgtccg cttacccacc acaaactgcc agaacaagat
     1861 ggcaggcgcc cagagcacga aggacagtac ccaggcagca gcaatcatga ggcctgccat
     1921 cttggtggtg cgccgggcag ggtaggtgag aggcttggtg acgcagaagt agcggtcaaa
     1981 gctgatgatg agaaggttca tgacggaggc gttgctcacc acgcagtcca gggccagcca
     2041 caggtcgcag accacggcgc ccaggggcca gtagcccttg atgatgtaca cggtgtagag
     2101 gttcatggag aaggcgccta tgatgagatc agcacacgcc aggctgaaga ggaagtagtt
     2161 gttgactgtc tgcagctgcc tgttgacctt gatggacagc atcaccagga tgttgcccac
     2221 gacagtcacc aggctcaggg agcctgtcac tgtggcaatg aagaccattt ccaccgtctc
     2281 atagcgattg tgggatgatg acgtgaccag gcgcacggac tgattgcccg agctgccatt
     2341 gacaggtgtg aagttggcca tggtggcgct agcgaagttc ctatactttc tagagaatag
     2401 gaacttcttg ccttaaccca gaaattatca ctgttattct ttagaatggt gcaaagagaa
     2461 gttcctatac cttttgaaga ataggaactt cgaattcgat atcaagctta tcgataatca
     2521 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     2581 tacgctatgt ggata
//
