LOCUS       sequence-299790-        2840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human synapsin-1 promoter; Flp-dependent KORD-IRES-mCitrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2840)
  TITLE     Unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSyn-fDIO-HA-KORD-IRES-mCitrine-WPREpA
FEATURES             Location/Qualifiers
     source          1..2840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..66
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(join(165..875,876..878,879..881))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(207..228)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     556..575
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     815..836
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      878..887
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    888..1461
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1048..1067)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     1277..1294
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             1975..1986
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(2620..2646)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2660..2693)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 aaggtaccgg atcctctaga gtcgactccg gagaagttcc tattctctag aaagtatagg
       61 aacttcgcag aatggtagct ggattgtagc tgctattagc aatatgaaac ctcttagaag
      121 ttcctattct tcaaaaggta taggaacttc ggcgcgccct atcacttgta cagctcgtcc
      181 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
      241 tcgttggggt ctttgctcag tttggactgg tagctcaggt agtggttgtc gggcagcagc
      301 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
      361 tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc
      421 atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg
      481 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
      541 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
      601 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
      661 cgggcgaagc acatcaggcc gtagccgaag gtggtcacga gggtgggcca gggcacgggc
      721 agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
      781 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
      841 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggctta tcatcgtgtt
      901 tttcaaagga aaaccacgtc cccgtggttc ggggggccta gacgtttttt taacctcgac
      961 taaacacatg taaagcatgt gcaccgaggc cccagatcag atcccataca atggggtacc
     1021 ttctgggcat ccttcagccc cttgttgaat acgcttgagg agagccattt gactctttcc
     1081 acaactatcc aactcacaac gtggcactgg ggttgtgccg cctttgcagg tgtatcttat
     1141 acacgtggct tttggccgca gaggcacctg tcgccaggtg gggggttccg ctgcctgcaa
     1201 agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca gaggaactgc ttccttcacg
     1261 acattcaaca gaccttgcat tcctttggcg agaggggaaa gacccctagg aatgctcgtc
     1321 aagaagacag ggccaggttt ccgggccctc acattgccaa aagacggcaa tatggtggaa
     1381 aataacatat agacaaacgc acaccggcct tattccaagc ggcttcggcc agtaacgtta
     1441 gggggggggg agggagaggg gcgatatcgc ggccgctcat actggtttat tcatcccatc
     1501 gatgtccctc aggtaagcag gatcctgaac tgtatttcgg actctgctag tgctctgccg
     1561 ctccatcctc atcttcagtg gaaagcagaa gtcccggaaa caccgcttga agttttcatc
     1621 aagaaaggcg tagagaatgg gattcaggct actgttggta tagcctaagg cgatgcagaa
     1681 gtaatagctg gagagagcag ctgtgctgtg ggaggtgctc cccagagcct ccaccaggat
     1741 gaatatgtga atgggagtcc agcagacgac gaagactgcc accaccacca ggaccagtct
     1801 ggtgatccta cgcaggttgc gatctttctc tcgggagcca gaaaggagcc ggacgctctt
     1861 gagacgcagg atcatcaggg tgtagcagac gatgatgatg aggacaggga tcacgaaggc
     1921 aaagatgaag acgcagatct tcatgaagag gtcccaccag gagtagtcat catctgggaa
     1981 ctgcaaggag cactcaatga catcgacgtc ttccctgact ttggtgcctc caaggactat
     2041 tgcagagatg ccaacagatg acgacagcag ccagatgcag atattgatga tctttgcctt
     2101 caagggtgtg cggaagtcca aagccttcac ggggtggcac acggcaatgt agcggtccac
     2161 gctcatcatg gtcaaggtga agatgctggt gaacatgttg tagtaattaa tggaaattac
     2221 tatcttgcac agcacatccc caaaaggcca ggaattcatc aagtagaccg tactctgaaa
     2281 gggcatggtt gtagtaacta aagcatctgc caaagccagg ttaaatatgt aaatgttggt
     2341 tgctgtcttc atctttgtgt atcggatgat cacgaacatg accagcgagt tgcccaccaa
     2401 gcccacgacg aacactacgg agtagaccgc cgtgatgatg accgggatgg ccggggagat
     2461 gtgcgcgggc tccagctgcg cgtcctccga gccggcgctg ccgttgctgt cgggctcggc
     2521 ccagccggga aaccaggcgc tgctgttggg gggcaggcag gcgctcgggg cgcaggtagg
     2581 gcccggctcc ccgcggaaga tctgaatcgg ggattccata gcgtaatctg gaacatcgta
     2641 tgggtacatg gtggctagcg aagttcctat actttctaga gaataggaac ttcttgcctt
     2701 aacccagaaa ttatcactgt tattctttag aatggtgcaa agagaagttc ctataccttt
     2761 tgaagaatag gaacttcgaa ttcgatatca agcttatcga taatcaacct ctggattaca
     2821 aaatttgtga aagattgact
//
